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A method for quantitatively detecting the amount of fish mesenteric fat deposition and its application

A quantitative detection and fat deposition technology, applied in the biological field, can solve the problems of time-consuming and laborious, error, intestinal rupture, etc., and achieve the effect of avoiding time-consuming and laborious, complete sampling, and accurate quantification.

Active Publication Date: 2022-07-19
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is simple and direct, it is prone to rupture of the intestinal tube during actual scraping, which makes the contents of the intestinal tract mixed with it, resulting in large errors in the data
Especially when the number of samples to be processed is large or the size of experimental fish is small, this method has problems such as time-consuming and labor-intensive, a high rate of operational errors, and difficult to standardize operations.

Method used

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  • A method for quantitatively detecting the amount of fish mesenteric fat deposition and its application
  • A method for quantitatively detecting the amount of fish mesenteric fat deposition and its application
  • A method for quantitatively detecting the amount of fish mesenteric fat deposition and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 provides a kind of detection method of quantitative fish mesenteric fat deposition , which includes the following steps:

[0054] S1. Take out the visceral mass of the fish, and remove the gallbladder organ;

[0055] S2, the visceral mass sample is rapidly dehydrated with absolute ethanol, and the sample is drained;

[0056] S3, the dehydrated sample is dyed with Oil Red O dye solution, and the sample is drained;

[0057] S4. Rinse with absolute ethanol to remove the residual dye solution on the surface of the sample, and drain the sample;

[0058] S5, put the sample of S4 into a sealed container filled with anhydrous ethanol solution, and carry out static extraction; centrifuge the obtained extract, and collect the supernatant;

[0059] S6, measure the absorbance OD value of the S5 supernatant at a wavelength of 510 nm;

[0060] S7. Compare the absorbance OD value of S6 with the absorbance OD value of the Oil Red O standard curve to obtain the Oil Red...

Embodiment 2

[0064] Example 2 Optimization of dehydration time, dyeing time and extraction time in the method of the present invention

[0065] 1, Optimisation of fast spin times

[0066] Since the dyeing and extraction of the samples are all in an anhydrous ethanol environment, the samples will be dehydrated by themselves. This may interfere with staining and may also affect the accuracy of extract quantification. Therefore, it is necessary to add a rapid sample dehydration step before the dyeing operation to effectively avoid the adverse effects that may be caused by the large dehydration of the sample during the dyeing or extraction process.

[0067] In order to explore the parameters of rapid dehydration processing time, the fixed samples of visceral mass were put into absolute ethanol for continuous detection. The test results are shown in Table 1. After soaking in absolute ethanol for 5 minutes, the average dehydration rate of the samples was 35.03%; after the soaking was exten...

Embodiment 3

[0078] Embodiment 3 provides a kind of quantitative analysis method that compares fish mesenteric fat deposition difference , the specific steps are as follows, such as figure 2 shown:

[0079] 1 Materials and methods

[0080] 1.1 Experimental fish

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Abstract

The invention provides a method and application for quantitatively detecting the fat deposition amount of fish mesentery. Based on the characteristic that the fat-soluble dye Oil Red O can specifically color adipose tissue, the invention develops an accurate and convenient method for quantifying the fat deposition amount in the mesentery of fish. The dyeing-extraction technical solution is adopted to avoid the problems of rough quantification, large errors, and inconvenient operation of the traditional method of directly weighing adipose tissue by scraping with a scalpel. , extraction and quantification, to obtain the deposition amount of individual mesenteric fat, which is expressed by the mass of Oil Red O adsorbed and extracted by the sample, which can be used to accurately compare the differences in mesenteric fat deposition among fish individuals.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and application for quantitatively detecting the amount of fat deposition in the mesentery of fish. Background technique [0002] In recent years, with the promotion and application of the high-yield, high-efficiency and intensive model, the growth rate of farmed fish has accelerated, and the market cycle has been shortened. Among them, the mesenteric adipose tissue in the abdominal cavity is the main functional part of most fish to store energy and accumulate fat. Excessive deposition not only causes waste of feed resources, but also directly exacerbates obesity and affects the sales of fresh products. [0003] In response to the above-mentioned industrial problems, researchers have tried to carry out research on the regulation mechanism and application technology from the aspects of nutrition, feed, genetics and breeding. In the process of research and develop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/30
CPCG01N21/31G01N1/30
Inventor 姜鹏樊佳佳李胜杰杜金星马冬梅雷彩霞
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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