Active protein extraction method

Abstract

The invention relates to an active protein extraction method. The method comprises the following steps of: S1, screening raw materials: selecting fresh fruit and vegetable seeds; S2, screening: screening the fruit and vegetable seeds to select seeds with germs; S3, activating: soaking the seeds with the germs in water for 12-20 hours; S4, cultivating the germs: putting the seeds with the germs into a nutrient solution for cultivating the seedlings for 72-96 hours; S5, removing the seedlings: screening and removing the germinated seeds, and leaving the non-germinated germ seeds; S6, pulping: performing wall-breaking pulping on the germ seeds obtained in the S5; S7, performing enzymolysis: performing enzymolysis on the obtained protein after pulping; S8, performing membrane separation: filtering through a membrane to intercept the protein; and S9, drying: performing freeze-drying or spray-drying on the intercepted mixture. The seeds with the germs are selected as the raw materials for protein purification, the activity of the protein in the germ seeds is relatively high, and extraction is performed through enzymolysis and membrane separation, so that the obtained protein is relatively high in purity and activity.

Description

technical field [0001] The present application relates to the technical field of protein extraction, in particular to a method for extracting active protein. Background technique [0002] Protein is an indispensable and important material basis in life activities, and it is the main form that human beings rely on for survival. In order to meet people's ever-improving living standards, it has become an urgent problem for the food industry to rationally develop protein resources, improve food quality and nutritional functions from the perspective of reasonable diet, nutritional balance and health. In recent years, with the deepening of people's understanding of the correlation between "diet and health", the importance of plant protein to maintain human health has also been recognized by the world, such as eating plant protein can reduce blood fat, prevent cancer, prevent heart disease Cerebrovascular diseases, etc., so more and more researchers focus on plant protein. [000...

AI Technical Summary

Problems solved by technology

[0004] In the above process of extracting vegetable protein, the protein is extracted by acid precipitation, and acidic substances are likely to remain in the extract, thereby affecting the purity of the protein