SNP marker molecule related to concentrated yellowing characters in tobacco mature period and application of SNP marker molecule

A technology for marking molecules, maturity stages, applied in the field of agricultural biology

Pending Publication Date: 2022-01-11
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the genetic law and related regulatory genes of the Nicotiana officinalis mutant in tobacco concentration.

Method used

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  • SNP marker molecule related to concentrated yellowing characters in tobacco mature period and application of SNP marker molecule
  • SNP marker molecule related to concentrated yellowing characters in tobacco mature period and application of SNP marker molecule
  • SNP marker molecule related to concentrated yellowing characters in tobacco mature period and application of SNP marker molecule

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Experimental program
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Effect test

Embodiment 1

[0056] Mapping of genes related to concentrated yellowing characteristics in tobacco mature stage and development of closely linked SNP markers 1. Construction of test materials

[0057] The test parent mutant Pale Yellow (PY), flue-cured tobacco variety NC95, and air-cured tobacco KY171 were provided by the National Tobacco Germplasm Bank. The mutant PY was used as the donor parent, and NC95 was used as the recurrent parent ( figure 1 ), the homozygous material PYNC95 was obtained by self-crossing after 5 generations of backcrossing; the homozygous material PYKY171 was obtained by self-crossing after 5 generations of backcrossing with the mutant PY as the donor parent and KY171 as the recurrent parent. Two pairs of near-isogenic lines, NC95, PYNC95 (CK1; NIL1) and KY171, PYKY171 (CK2; NIL2), were constructed for gene mapping. The specific methods are as follows.

[0058] The experiments were carried out in the greenhouse of the Jimo experimental base of the Tobacco Research ...

Embodiment 2

[0087] Cultivation Method of New Variety of Flue-cured Tobacco with Concentrated Falling Yellow

[0088] The molecular marker SNP-660961 (PCR product 376bp) that is closely linked to the concentrated yellow fall of tobacco at the maturity stage by using the characteristics described above, using K326 as the recurrent parent, obtained a new line of concentrated yellow fall after multiple backcrosses, and there is no obvious difference in maintaining other traits Under normal circumstances, the field shows concentrated yellowing in the late stage of maturity, and can be harvested 2 to 3 times.

[0089] The specific cultivation process is as follows:

[0090] In the spring of 2014, K326 was used as the female parent and the mutant PYNC95 was used as the male parent to configure hybrid combinations to obtain F1 hybrid seeds; in the winter of 2014, K326 was used as the recurrent parent in the greenhouse to configure BC 1 f 1 Separate groups.

[0091] In the spring of 2015, the u...

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Abstract

The invention provides an SNP marker molecule related to concentrated yellowing characters in a tobacco mature period and application of SNP marker molecule, and belongs to the technical field of agricultural biology. According to the SNP marker molecule related to the concentrated yellowing characters in the tobacco mature period, an A/T polymorphic site exists at the 160th site on the basis of a nucleotide sequence shown in SEQ ID NO:1. The SNP molecular marker is closely linked with a mature-stage concentrated yellowing gene Ntpy gene. The SNP molecular marker is used for cultivating a new variety of concentrated yellowing flue-cured tobacco, the new variety PYK326 of concentrated yellowing flue-cured tobacco is successfully screened by screening flue-cured tobacco offspring single plants with genotypes of AA or AT and combining the characteristics of the growth vigor of offspring plants, the botanical character and agronomic character of the new variety PYK326 are basically consistent with those of a parent K326, the maturation time is advanced by one week, and the new variety is concentrated yellowing flue-cured tobacco. Therefore, the SNP marker molecule provided by the invention provides a new screening means for the cultivation of new varieties of flue-cured tobaccos.

Description

technical field [0001] The invention belongs to the technical field of agricultural biotechnology, and specifically relates to a SNP marker molecule related to the concentrated yellowing trait of tobacco at maturity stage and its application. Background technique [0002] Tobacco (Nicotiana tabacum L.) is a foliar economic crop, and the natural degradation of chlorophyll in its tobacco leaves during the mature stage is called "falling yellow" [1] . Because tobacco leaves generally fall yellow layer by layer from bottom to top. [2] , Normal tobacco plants need more than a month to turn yellow from the bottom to the top leaves successively, and 3 to 5 harvests are carried out, which greatly increases the workload of tobacco production. Therefore, improving the maturity period of tobacco leaves and increasing the concentrated maturity of tobacco leaves is of great significance for reducing the labor consumption of tobacco leaf harvesting, baking and other production links. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 陈明丽张银霞龚达平张新要李东徽蒲文宣周东波解敏敏李志远杨小年
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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