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Electrophoresis and electrotransfer devices, systems, and methods

A technology of electrotransfer and electrophoresis box, which is applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., and can solve problems such as leakage, buffer use, and buffer leakage

Pending Publication Date: 2022-01-14
LIFE TECH CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each procedure has multiple time-consuming steps that require different sets of equipment
In addition, existing electrophoresis systems often have problems with the use of large amounts of buffer, such as buffer overflow and / or overflow and / or leakage of buffer from the anode and cathode buffer reservoirs
Similar problems exist with state-of-the-art wet electrotransfer systems, including buffer overflow, and / or buffer leakage during assembly and operation, large amounts of buffer usage and waste during stack assembly, etc.

Method used

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  • Electrophoresis and electrotransfer devices, systems, and methods
  • Electrophoresis and electrotransfer devices, systems, and methods
  • Electrophoresis and electrotransfer devices, systems, and methods

Examples

Experimental program
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Effect test

example 1

[0243] Example 1: Electrophoresis using different gel chemistries

[0244] The electrophoresis systems and devices of the present invention are used to perform electrophoresis on several different gel chemistries and formulations. Table 1 provides some non-limiting exemplary run conditions during electrophoresis for various types of gel chemistry for gels cast as midigels. The current values ​​in the table below are for one gel. If running two gels, the current will be doubled. Note that current and line time values ​​are approximate and will vary with gel percentage and power source used for electrophoresis.

[0245] Table 1:

[0246]

example 2

[0247] Example 2: Performance of electrotransfer systems, devices and methods compared to existing electrotransfer devices

[0248] In electrotransfer experiments, the electrotransfer system of the present invention was compared to Bio-Rad's Criterion tank transfer (electrotransfer) system. In some embodiments, the electrotransfer systems of the present invention perform electrotransfer of proteins at much lower voltages, currents, and powers than Bio-Rad Criterion cells. In one example, electrotransfer was performed using a NuPAGE midi gel on two systems with the following results:

[0249] Electrotransfer in the system of the present invention is performed at 25V / 725mA / 18.1W (Note: 25V*0.725A=18.1W)

[0250] In contrast, electrotransfer in Bio-Rad's Criterion tank blotter requires much higher voltage and power (100V / 930mA / 93.0W).

[0251] Thus, the electrotransfer system of the present invention can use lower power and thus less expensive power supply units than existing s...

example 3

[0252] Example 3: Assembly and performance of electrotransfer systems and devices

[0253] In the electrotransfer experiments, the electrotransfer system of the present invention was compared with the Thermo Fisher Microblotting Module B1000, which is an existing electrotransfer system, to analyze liquid-tight seals by conducting experiments to study comparative leak rates. The timed leak test is performed as follows:

[0254] 1. Wetting the electrotransfer system unit to open and close the device while operating underwater. Make sure the unit is wetted before each leak test.

[0255] 2. Build the electrotransfer stack and completely soak all parts in water before taking measurements:

[0256] 1) 1) Moisten the sponge (qty 2).

[0257] 2) Moisten filter paper (qty 4)

[0258] 3) Assemble the electrotransfer stack in the following order: sponge (x1), filter paper (x4), and sponge (x1)

[0259] 4) Roll each layer with a transfer roller, removing any air bubbles between each...

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Abstract

The invention provides a system for gel electrophoresis and electrotransfer. The system includes one or more chambers. The one or more chambers can removably and interchangeably receive an electrophoresis cartridge or an electrotransfer cartridge, and provide an electrical interface for both electrophoresis and electrotransfer of biomolecules. The invention also provides an electrophoresis device comprising the clamp and the electrotransfer box, and a related device. Methods for electrophoresis and electrotransfer using the systems and devices of the invention are also provided.

Description

technical field [0001] The present invention generally relates to systems that can perform both electrophoresis and electrotransfer of biomolecules. The invention further relates to an electrophoretic system, an electrophoretic device, an electrotransfer system and an electrotransfer device. Methods of electrophoresis and electrotransfer using these systems and devices are also described. Background technique [0002] Electrophoresis is a commonly used method for separating biological molecules (biomolecules) such as nucleic acids, DNA, RNA, peptides and proteins based on their size and charge. In gel electrophoresis, biomolecules are separated into bands by an electric field, which causes the molecules to migrate through a filter matrix. Typical filter matrices include gels. Electrophoresis cassettes consist of a strip of filter matrix, such as gel, sandwiched between two glass or plastic plates. Gels have an open molecular network structure that defines pores saturated...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447G01N27/44704G01N27/44782G01N27/44713G01N27/44739G01N27/44743
Inventor M·村上K·布洛赫N·奥尔森R·温尼克张维富M·撒克
Owner LIFE TECH CORP
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