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Construction method of tilapia ventral fin cell line

A construction method and technology of tilapia, applied in the field of cell biology, can solve the problems that the construction of tilapia pelvic fin cell lines has not been reported yet, and achieve the effect of strong cell reproduction ability

Pending Publication Date: 2022-01-18
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the existing problems and defects of the prior art are: the construction of the tilapia pelvic fin cell line has not been reported in the prior art

Method used

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  • Construction method of tilapia ventral fin cell line
  • Construction method of tilapia ventral fin cell line
  • Construction method of tilapia ventral fin cell line

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Embodiment Construction

[0034] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0035] Aiming at the problems existing in the prior art, the present invention provides a method for constructing a tilapia pelvic fin cell line. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0036] Such as figure 1 As shown, the construction method of the tilapia pelvic fin cell line provided by the embodiments of the present invention comprises the following steps:

[0037] S101, configuring the cell culture medium;

[0038] S102, performing pretreatment of the tilapia pelvic fin cell block;

[0039] S103, performing primary culture of tilapia pelvic fin cells;...

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Abstract

The invention belongs to the technical field of cell biology, and discloses a construction method of a tilapia ventral fin cell line. The construction method of the tilapia ventral fin cell line comprises the following steps of preparing a cell culture medium; pretreating tilapia ventral fin cell blocks; carrying out primary culture on the pelvic fin cells of the tilapia; and carrying out subculture on the pelvic fin cells of the tilapia. According to the construction method of the tilapia ventral fin cell line, the culture medium suitable for growth and proliferation of the cells and suitable culture conditions are optimized according to the characteristics of the tilapia ventral fin cells, the tilapia ventral fin cell line is successfully constructed, and the cells are high in reproductive capacity, the method can be applied to important research on related functional genes of cytobiology, molecular biology, virology, toxicology, oncology, genetics, immunology and the like, and provides a basis for developing fish virus infection ways and infection mechanisms on the molecular cell level and developing viral vaccines and the like.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a method for constructing a tilapia pelvic fin cell line. Background technique [0002] At present, after years of development of fish cell lines, various freshwater and seawater fish cell lines have become important in vitro research systems in various disciplines. Many important experiments and new technologies require further research on the basis of fish cell lines. Research. The scope of application of fish cell lines is becoming more and more extensive, and has expanded from the initial virus isolation, purification, identification, and in vitro propagation to the toxicity mechanism of environmental pollutants and its monitoring, fish immunity, germplasm improvement, resource protection and many other fields. Fish cell lines can not only be used as a good in vitro research system for basic research in various disciplines such as cell biology, fish virology...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12R1/91
CPCC12N5/0602C12N2509/00C12N2509/10C12N2500/38C12N2500/60C12N2501/11
Inventor 黄瑜简纪常郑哲宋海霞宋长江蔡佳王蓓汤菊芬
Owner GUANGDONG OCEAN UNIVERSITY
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