Construction method of tilapia ventral fin cell line

Abstract

The invention belongs to the technical field of cell biology, and discloses a construction method of a tilapia ventral fin cell line. The construction method of the tilapia ventral fin cell line comprises the following steps of preparing a cell culture medium; pretreating tilapia ventral fin cell blocks; carrying out primary culture on the pelvic fin cells of the tilapia; and carrying out subculture on the pelvic fin cells of the tilapia. According to the construction method of the tilapia ventral fin cell line, the culture medium suitable for growth and proliferation of the cells and suitable culture conditions are optimized according to the characteristics of the tilapia ventral fin cells, the tilapia ventral fin cell line is successfully constructed, and the cells are high in reproductive capacity, the method can be applied to important research on related functional genes of cytobiology, molecular biology, virology, toxicology, oncology, genetics, immunology and the like, and provides a basis for developing fish virus infection ways and infection mechanisms on the molecular cell level and developing viral vaccines and the like.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a method for constructing a tilapia pelvic fin cell line. Background technique [0002] At present, after years of development of fish cell lines, various freshwater and seawater fish cell lines have become important in vitro research systems in various disciplines. Many important experiments and new technologies require further research on the basis of fish cell lines. Research. The scope of application of fish cell lines is becoming more and more extensive, and has expanded from the initial virus isolation, purification, identification, and in vitro propagation to the toxicity mechanism of environmental pollutants and its monitoring, fish immunity, germplasm improvement, resource protection and many other fields. Fish cell lines can not only be used as a good in vitro research system for basic research in various disciplines such as cell biology, fish virology...

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the existing problems and defects of the prior art are: the construction of the tilapia pelvic fin cell line has not been reported in the prior art

Images