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Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof

A detection method and polymorphism technology, applied in the field of molecular biology, can solve the problems of complex operation process, long detection period and high detection cost, and achieve the effects of high signal, reduction of primer dimers and strong specificity.

Pending Publication Date: 2022-01-28
郑州华沃生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result of direct sequencing method is more accurate, but its operation process is more complicated, time-consuming and costly
The ARMS PCR method uses the method of relative Ct difference to determine the genotype, and it is impossible to distinguish the genotype through one tube
The gene chip method and the liquid phase chip method also have complicated operation process, high detection cost and long detection cycle

Method used

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  • Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof
  • Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof
  • Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof

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Embodiment

[0036] Example: A detection method for detecting ADRB1 gene G1165C polymorphism

[0037] The method of the present invention adopts the rhAmp SNP typing technology and improves it on the basis of it. It is based on RNaseH2-dependent PCR (rhPCR), which can provide high signal and specificity for SNP analysis. rhAmp SNP Genotyping combines a unique two-enzyme system with 3' end-blocked DNA-RNA hybrid primers to detect SNP loci. Activation of a blocked primer occurs after hybridization to its perfectly matched target, thereby eliminating or greatly reducing primer-dimers. The thermostable hot-start RNase H2 immediately cleaves the primer 5' of the ribose, releasing the blocking group and allowing primer extension. The method of the present invention only needs two typing probe primers and one universal primer to realize the typing of the SNP site.

[0038] The specific sequence structure of the typing probe primer is as follows:

[0039] Reporter-DDDDDDDDDDDDDDDDDDrDDDDM-NFQ. ...

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Abstract

The invention discloses a primer and a kit for detecting ADRB1 gene G1165C polymorphism and a detection method and application thereof. The typing probe primer for detecting the G1165C polymorphism of an ADRB1 gene is designed, and the sequence structure of the typing probe primer is as follows: A-DDDDDDDDDDDDDDDDDDDDrDDDDM-B. The invention provides the kit for detecting the G1165C polymorphism of the ADRB1 gene, and also provides the detection method for the G1165C polymorphism of the ADRB1 gene. Primer dimers can be eliminated or greatly reduced, the primer is immediately cut at the 5'position of ribose through thermal-stable hot start RNase H2, a closed group is released, the primer is allowed to extend, SNP site typing can be achieved only through two typing probe primers and one universal primer, and during detection, the signal is high, and specificity is high.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a primer, a kit, a detection method and application thereof for detecting the G1165C polymorphism of the ADRB1 gene. Background technique [0002] As a chronic non-communicable disease, hypertension is a chronic disease with high prevalence, high disability rate and heavy disease burden in my country. Compared with developed countries, the number of people suffering from hypertension in my country is large. Although the awareness rate, treatment rate and control rate of hypertension have increased in recent years, they are still at a low level. The prevention and control of chronic diseases pose great challenges. [0003] At present, the clinical methods for controlling hypertension are mainly non-pharmaceutical measures such as changing eating habits and lifestyle and drug treatment. Among them, drug therapy is the most effective measure to control blood pressure. Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2563/107C12Q2521/327C12Q2527/125Y02A50/30
Inventor 王知丰秦付军吴书展
Owner 郑州华沃生物科技有限公司
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