Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof

Abstract

The invention discloses a primer and a kit for detecting ADRB1 gene G1165C polymorphism and a detection method and application thereof. The typing probe primer for detecting the G1165C polymorphism of an ADRB1 gene is designed, and the sequence structure of the typing probe primer is as follows: A-DDDDDDDDDDDDDDDDDDDDrDDDDM-B. The invention provides the kit for detecting the G1165C polymorphism of the ADRB1 gene, and also provides the detection method for the G1165C polymorphism of the ADRB1 gene. Primer dimers can be eliminated or greatly reduced, the primer is immediately cut at the 5'position of ribose through thermal-stable hot start RNase H2, a closed group is released, the primer is allowed to extend, SNP site typing can be achieved only through two typing probe primers and one universal primer, and during detection, the signal is high, and specificity is high.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a primer, a kit, a detection method and application thereof for detecting the G1165C polymorphism of the ADRB1 gene. Background technique [0002] As a chronic non-communicable disease, hypertension is a chronic disease with high prevalence, high disability rate and heavy disease burden in my country. Compared with developed countries, the number of people suffering from hypertension in my country is large. Although the awareness rate, treatment rate and control rate of hypertension have increased in recent years, they are still at a low level. The prevention and control of chronic diseases pose great challenges. [0003] At present, the clinical methods for controlling hypertension are mainly non-pharmaceutical measures such as changing eating habits and lifestyle and drug treatment. Among them, drug therapy is the most effective measure to control blood pressure. Th...

AI Technical Summary

Problems solved by technology

The result of direct sequencing method is more accurate, but its operation process is more complicated, time-consuming and costly

The ARMS PCR method uses the method of relative Ct difference to determine the genotype, and it is impossible to distinguish the genotype through one tube

The gene chip method and the liquid phase chip method also have complicated operation process, high detection cost and long detection cycle

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