Primer and kit for detecting ADRB1 gene G1165C polymorphism and detection method and application thereof
A detection method and polymorphism technology, applied in the field of molecular biology, can solve the problems of complex operation process, long detection period and high detection cost, and achieve the effects of high signal, reduction of primer dimers and strong specificity.
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[0036] Example: A detection method for detecting ADRB1 gene G1165C polymorphism
[0037] The method of the present invention adopts the rhAmp SNP typing technology and improves it on the basis of it. It is based on RNaseH2-dependent PCR (rhPCR), which can provide high signal and specificity for SNP analysis. rhAmp SNP Genotyping combines a unique two-enzyme system with 3' end-blocked DNA-RNA hybrid primers to detect SNP loci. Activation of a blocked primer occurs after hybridization to its perfectly matched target, thereby eliminating or greatly reducing primer-dimers. The thermostable hot-start RNase H2 immediately cleaves the primer 5' of the ribose, releasing the blocking group and allowing primer extension. The method of the present invention only needs two typing probe primers and one universal primer to realize the typing of the SNP site.
[0038] The specific sequence structure of the typing probe primer is as follows:
[0039] Reporter-DDDDDDDDDDDDDDDDDDrDDDDM-NFQ. ...
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