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Molecular marker related to oat yield and application thereof

A technology of molecular markers and oats, applied in the field of molecular genetics, to achieve the effect of high application value

Pending Publication Date: 2022-01-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the discovery of relevant markers depends on the cloning of genes, the correct selection of materials, relevant technical experience, etc., and even the cloning of relevant genes does not guarantee that useful markers can be discovered, so this kind of research not only has technical requirements, material requirements, but also highly unpredictable

Method used

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  • Molecular marker related to oat yield and application thereof
  • Molecular marker related to oat yield and application thereof
  • Molecular marker related to oat yield and application thereof

Examples

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Effect test

Embodiment 1

[0041] Example 1 The acquisition of a SNP molecular marker that is significantly correlated with the thousand-grain weight of the oat GW2 gene and the determination of its detection primers

[0042] A SNP molecular marker that is significantly correlated between the oat GW2 gene and the thousand-grain weight of the present invention is obtained by the following method:

[0043] 1) A total of 96 oat germplasm resources from different geographical sources were collected, and the associated groups used for mapping were constructed. This population is rich in genetic diversity.

[0044] 2) Determination of thousand-grain weight of related populations. From 2018 to 2020, the collected materials were planted in the Changping Experimental Base of the Chinese Academy of Agricultural Sciences, the Bashang Experimental Station of the Zhangjiakou Academy of Agricultural Sciences, and the Changji Experimental Station of the Xinjiang Academy of Agricultural Sciences, under normal experime...

Embodiment 2

[0052] Embodiment 2 The application experiment of the SNP marker of the present invention on the oat thousand-grain weight

[0053] 1) Detection of SNP markers in some oat varieties.

[0054] In order to further verify the effectiveness of the molecular marker of the present invention, the present invention randomly selects 24 parts of oat varieties in the associated population of Example 1, uses its DNA as a template, and utilizes competitive allele-specific PCR (Kompetitive AlleleSpecific PCR), namely KASP technology, based on the SNPline genotyping technology platform of LGC Genomics, performs SNP detection on the samples to be tested.

[0055] The specific method is: randomly select 24 parts of oat varieties, use oat genome DNA as a template, and use specific primers (SEQ ID NO. , the 5' end of which is connected with a HEX fluorescent group (SEQ ID NO.4) is used as a primer, and the SNP detection of the sample to be detected is carried out by using the competitive allele...

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Abstract

The invention relates to the technical field of molecular genetics, in particular to a molecular marker related to oat yield and application of the molecular marker. The molecular marker related to the oat yield, provided by the invention, contains a nucleotide sequence of which the polymorphism at the 274th site is A / G, wherein the nucleotide sequence is shown as SEQ ID NO.1. When the polymorphic site of the molecular marker is A, the weight of the oat grains is large; and when the polymorphic site of the molecular marker is G, the weight of the oat grains is small. The molecular marker provided by the invention can be used for screening oats with high thousand grain weight, and plays an important role in molecular-assisted breeding of high-yield oats.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to molecular markers related to oat yield and applications thereof. Background technique [0002] Oats are rich in nutrients. Compared with wheat, rice, corn and other crops, oats are in the forefront of nutritional indicators such as protein, unsaturated fatty acids, vitamins, and mineral elements. Therefore, oats are one of the best complete nutritional foods among grains. . Oatmeal is a recognized health food. In recent years, with the improvement of people's living standards and the promotion of oat nutrition and health care knowledge, oats are not only the consumption rations of residents in the production areas, but also functional foods for people to balance nutrition and implement scientific diets. The demand for oat products is increasing. However, due to the backward level of oat breeding, the oat yield is low, and the average yield per mu is only 300-400 catt...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107Y02P60/87
Inventor 吴斌
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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