Electrochemical synthesis method and application of D-nucleoside compound
A synthesis method and compound technology are applied in the field of electrochemical synthesis of D-nucleoside cooling agents to achieve the effects of enriching tobacco aroma, reducing substance consumption and reducing side reactions
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Embodiment 1
[0032] Example 1: (-)-L-menthyl-D-nucleoside
[0033] Substrate 150mg (1mmol) D-ribose and 156mg (1mmol) (-)-L-menthol are standard substrates, under constant current conditions (J=15mA / cm 2 ), without separating the electrolyzer, to NH 4 Br is the supporting electrolyte, CH 3 CN / H 2 When O was used as the electrolyte and the Pt electrode was used as both the anode and the cathode, the reaction was carried out at room temperature for 5 hours. After extraction, the target product was obtained by column chromatography with a yield of 85%.
[0034] The product structure is as figure 2 .
[0035] The NMR spectrum of the product obtained is as follows:
[0036] α-Pyranose
[0037] 1 H NMR (CD 3 COCD 3 ,500MHz)δ=4.72(d,1H,J=3.0Hz,CH),3.88-3.74(m,3H,2xCH,CH 2 ),3.66-3.60(m,2H,CH,CH 2 ), 3.38(td, 1H, J=4.4, 10.7, CH), 2.34(ddd, 1H, J=14.0, 7.4, 2.7Hz, CH), 2.25-2.19(m, 2H, 2xCH), 1.72-1.02( m,6H,2xCH,2xCH 2 ),0.94-0.89(m,6H,2xCH 3 ),0.80(d,J=3.0Hz,3H,CH 3 )
[0038] ...
Embodiment 2
[0043] Example 2: Cyclopentyl-D-nucleosides
[0044] Substrate 150mg (1mmol) D-ribose and 26mg (1mmol) cyclopentanol were standard substrates, under constant current conditions (J=15mA / cm 2 ), without separating the electrolyzer, to NH 4 Br is the supporting electrolyte, CH 3 CN / H 2 When O was used as the electrolyte and the Pt electrode was used as both the anode and the cathode, the reaction was carried out at room temperature for 5 hours. After extraction, column chromatography separated the target product with a yield of 90%.
[0045] The product structure is as follows image 3 .
[0046] The NMR spectrum of the product obtained is as follows:
[0047] α-Pyranose
[0048] 1 H-NMR (CD 3 OD,500MHz)δ=4.77(d,1H,J=3.6Hz,CH),4.22-4.28(m,1H,CH),3.86-3.74(m,2H,CH,CH 2 ),3.65-3.59(m,2H,CH,CH 2 ),3.50(m,1H,CH),1.9-1.5(m,8H,4xCH2).
[0049] 13 C-NMR (CD 3 OD,126MHz)δ=100.2,80.4,73.1,69.9,68.9,64.4,33.9,32.5,24.1,23.9.
[0050] β-Pyranose
[0051] 1 H-NMR (D 2 O,500M...
Embodiment 3
[0054] Example 3: 2-Bromoethyl-D-ribopyranoside
[0055] Substrate 150mg (1mmol) D-ribose and 125mg (1mmol) 2-bromoethanol were standard substrates, under constant current conditions (J=15mA / cm 2 ), without separating the electrolyzer, to NH 4 Br is the supporting electrolyte, CH 3 CN / H 2 When O was used as the electrolyte and the Pt electrode was used as both the anode and the cathode, the reaction was carried out at room temperature for 5 hours. After extraction, column chromatography separated the target product with a yield of 78%.
[0056] The product structure is as follows Figure 4 .
[0057] The NMR spectrum of the product obtained is as follows:
[0058] α-Pyranose
[0059] 1 H-NMR (CD 3 OD,500MHz)δ=4.7(d,1H,J=2.9Hz,CH),4.02-3.96(m,1H,CH),3.86(m,1H,CH 2 ),3.86-3.76(m,3H,CH,CH 2 ),3.71-3.66(m,1H,CH 2 ),3.64-3.59(m,1H,CH),3.45(t,2H,J=6.1Hz,CH 2 ).
[0060] 13 C-NMR (CD 3 OD,126MHz)δ=100.6,72.4,70.1,69.8,68.5,64.7,31.4.β-Pyranose
[0061] 1 H-NMR (CD 3...
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