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Application of (-)-jorunnamycin A in inhibiting growth of bladder tumor cells and application method of (-)-jorunnamycin A

A technology for bladder tumors and application methods, applied in the application field of inhibiting the growth of bladder tumor cells, to achieve the effect of inhibiting the growth of bladder cancer cells and having a good inhibitory effect

Pending Publication Date: 2022-02-08
JINING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the anti-cancer compounds from marine sources have not been well developed, it is of great significance to research and develop new anti-tumor drugs based on marine sources

Method used

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  • Application of (-)-jorunnamycin A in inhibiting growth of bladder tumor cells and application method of (-)-jorunnamycin A
  • Application of (-)-jorunnamycin A in inhibiting growth of bladder tumor cells and application method of (-)-jorunnamycin A
  • Application of (-)-jorunnamycin A in inhibiting growth of bladder tumor cells and application method of (-)-jorunnamycin A

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Experimental program
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Effect test

Embodiment 1

[0040] The small molecule (-)-jorunnamycin A of the marine biological extract mentioned in the present invention has the following chemical structural formula:

[0041]

[0042] Regarding the inhibitory effect of (-)-jorunnamycin A on different types of cells, the inventors conducted the following experiments:

[0043] Take human embryonic kidney cells H293T, human colon cancer cells SW480, human glioma cells U251, bladder cancer cells SW780, bladder cancer cells T24, and bladder cancer cells UMUC3 as samples, and culture them in 96-well plates at an initial density of 25%. , when the density grew to 50-55%, different amounts of (-)-jorunnamycin A were added to make the final concentrations respectively 0, 20, 40, 60, 80, 100, 200, 300, 400 nM.

[0044] After culturing for 24 hours, the residual viability of the cells was detected by the CCK8 method and plotted (the cell viability without (-)-jorunnamycinA was taken as 100%).

[0045] attached figure 1 It is a diagram sho...

Embodiment 2

[0050] In Example 2, the present inventors further investigated the inhibitory effect of (-)-jorunnamycin A on muscle-invasive bladder cancer, and the specific steps are as follows:

[0051] Take a cell sample and culture it into a 96-well plate according to the initial density of 25%. When the density grows to 50-55%, add (-)-jorunnamycin A at a concentration of 0-200nM. After culturing for 24 hours, use the CCK8 method to detect the cells Remaining viability, and the half-inhibitory concentration IC50 of (-)-jorunnamycin A for the muscle-invasive bladder cancer cell line T24 was 54.1nM, and the half-inhibitory concentration IC50 for the muscle-invasive bladder cancer cell line UMUC3 was 83.6nM, as attached figure 2 shown.

Embodiment 3

[0053] The present invention further investigates (-)-jorunnamycin A to inhibit the division ability of muscle-invasive bladder cancer cell T24 cell, and the results are shown in the attached image 3 shown.

[0054] The specific method is as follows: One of the main characteristics of cancer cells is rapid mitosis, and the premise of cell mitosis is DNA replication. Therefore, the effect on DNA replication rate is an important experimental method to evaluate the tumor suppressor effect of drugs. In the present invention, the EdU kit method (the EdU kit is purchased from Beyuntian Company) is used to detect the inhibitory effect of (-)-jorunnamycin A on the DNA replication of bladder cancer cells. From attached image 3 It can be seen from the results in that (-)-jorunnamycin A can effectively inhibit the DNA synthesis of bladder cancer cells in a concentration-dependent manner.

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to application of (-)-jorunnamycin A in inhibiting the growth of bladder tumor cells and an application method of (-)-jorunnamycin A. The invention mainly provides the application of (-)-jorunnamycin A in inhibiting the growth of bladder tumor cells, in particular to the application of (-)-jorunnamycin A in inhibiting the growth of muscular infiltration type bladder cancer cells. When the (-)-jorunnamycin A is used, the (-)-jorunnamycin A is dissolved in an organic solvent, then the use concentration of the (-)-jorunnamycin A is kept between 150 nM and 400 nM, and the (-)-jorunnamycin A is administrated in an injection mode. The micromolecular (-)-jorunnamycin A derived from a marine organism extract provided by the invention can specifically inhibit proliferation of the bladder tumor cells, especially has a special effect on muscle layer infiltration type bladder cancer, can completely inhibit the growth of the bladder tumor cells at a concentration of 200 nM, and has an acceptable effect on normal cells; and results of researches on toxicity and curative effects on mice show that (-)-jorunnamycin A with a concentration of 600 [mu]g / kg has no obvious lethal toxic and side effects on the mice and has a good inhibition effect on bladder tumors transplanted to nude mice.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application and application method of (-)-jorunnamycin A in inhibiting the growth of bladder tumor cells. Background technique [0002] Bladder cancer is a common cancer worldwide, especially among men, and is the fourth most common cancer among men in the world. There are two types of bladder cancer: non-muscle-invasive bladder cancer and muscle-invasive bladder cancer. Among them, muscle-invasive bladder cancer is the most harmful, prone to lymphatic metastasis, bone metastasis, prostate metastasis or uterine metastasis, and the 5-year survival period is short. [0003] At present, in the aspect of bladder cancer, although there are surgery, BCG, chemotherapy drugs, immunotherapy, combined therapy and other means, surgery combined with chemotherapy is still the most mainstream treatment. During chemotherapy, cisplatin, gemcitabine, doxorubicin, epirubicin, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4995A61P35/00
CPCA61K31/4995A61P35/00
Inventor 陈瑞蛟夏勇韩心怡江利青马莉
Owner JINING MEDICAL UNIV