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Experimental method for treating POI by combining Rg1 with hAD-MSCs

An experimental method, the technology of had-mscs, is applied in the field of Rg1 combined with hAD-MSCs in the treatment of POI, which can solve the problems of unsatisfactory transplantation effect and low transplantation homing rate, and achieves a convenient operation, solves clinical problems, and has good promotion prospects. Effect

Pending Publication Date: 2022-02-08
何连利
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Problems solved by technology

Studies have shown that MSCs can restore the fertility function of chemotherapy-induced infertility patients and can differentiate into ovarian granulosa cells, and in vitro experiments have confirmed that they have the potential to differentiate into follicle-like cells, but their transplantation homing rate is low and their differentiation is limited. The unsatisfactory rate and transplantation effect limit its application

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  • Experimental method for treating POI by combining Rg1 with hAD-MSCs

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Embodiment

[0020] An experimental method for treating POI with Rg1 combined with hAD-MSCs, comprising the following steps:

[0021] 1) Culture of human amniotic mesenchymal stem cells hAD-MSCs: hAD-MSCs cells were cultured in RPMI 1640 culture medium containing 10% fetal bovine serum FBS, and 1% penicillin-streptomycin was added, and filled with 5% The cells were cultured in a humid 37°C constant temperature cell incubator with CO2, and the cells were continuously passaged at the third generation for subsequent experiments;

[0022] 2) Identification of hAD-MSCs by flow cytometry: Flow cytometry analysis was performed on the surface markers of hAD-MSCs cultured at passage 3 to determine their stem cell characteristics, and the cells were harvested and centrifuged at 250×g at room temperature 5min, then resuspend the cell pellet in PBS to prepare a single cell suspension, and make the cell concentration 1×105 / ml, the cells were mixed with anti-human CD-133, anti-human CD-45, anti-human CD...

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Abstract

The invention relates to an experimental method for treating POI by combining Rg1 with hAD-MSCs. The experimental method specifically comprises the following steps: 1) culturing human amniotic mesenchymal stem cells hAD-MSCs; 2) identifying the hAD-MSCs by flow cytometry; and 3) constructing the POI rat model with the ovarian insufficiency through radiation induction. The invention has the advantages that the scheme design is reasonable, the implementation operation is convenient, the clinical problem can be solved, and the popularization prospect is good.

Description

technical field [0001] The invention relates to an experimental method for treating POI with Rg1 combined with hAD-MSCs. Background technique [0002] Premature ovarian failure (POF) refers to the occurrence of non-physiological amenorrhea for at least 4 months after puberty before the age of 40, and two or more serum follicle-stimulating hormone (FSH) > 40μ / L (the interval between two inspections is more than 1 month), serum estradiol (estradiol, E2) <73.2pmol / L [1] , the main clinical manifestations are (1) primary or secondary infertility, (2) low estrogen symptoms: such as hot flashes, sweating, decreased concentration, loss of libido, dyspareunia, vaginal dryness, and different degrees of reproductive Organ and breast atrophy, etc. (3) Long-term complications: osteoporosis, cardiovascular disease, senile dementia, etc. The pathogenesis of POF is still unclear, but most scholars believe that its pathogenesis is related to the migration of germ cells, the initiat...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K31/704A61P15/00
CPCA61K35/28A61K31/704A61P15/00A61K2300/00
Inventor 何连利刘晓云谢红梅牟大英莫晓莉张青云祝叶路祥会
Owner 何连利