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Efficient decoloring method of amino acid fermentation liquor

A fermentation broth and amino acid technology is applied in the field of decolorization of amino acid fermentation broth, which can solve the problems of inability to remove precursor substances, affect product quality, dark color of fermentation broth, etc., and achieve the effects of improving overall decolorization efficiency, economical convenience, and simple process.

Active Publication Date: 2022-02-08
ANHUI HUAHENG BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If these pigments are not removed, the color of the fermentation broth will be dark, which will affect the quality of the product
[0003] In the prior art, the pigment content is mainly reduced by decolorizing the amino acid fermentation broth with activated carbon and other processes, but the above-mentioned process cannot remove the precursor substances that generate pigment in the fermentation broth, resulting in limited decolorization efficiency

Method used

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  • Efficient decoloring method of amino acid fermentation liquor
  • Efficient decoloring method of amino acid fermentation liquor

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Effect test

preparation example Construction

[0041] Preparation method of seed culture solution:

[0042] (1) Brevibacterium flavum (preservation number: CCTCC NO: M 2019496) preserved in a 1 mL glycerol tube was inoculated into 50 mL of LB medium, and cultured on a shaker at 37°C and 210 r / min until pH 6.2-6.5, OD The value is 3-4, and the LB seed solution is obtained;

[0043] (2) Take 5 mL of LB seed liquid, inoculate it into 200 mL of synthetic medium, and culture it on a shaker at 37°C and 210 r / min until pH 6.4-6.6 and OD value is 3-4 to obtain Seed culture fluid.

Embodiment 1

[0045] (1) Inoculate the seed culture solution into 10L fermentation medium according to the inoculum size of 2%, ferment and cultivate it at 35°C and pH6-7 until the OD value of the solution is 3, add 0.15g of sterilized sodium erythorbate, and continue Cultivate, stop fermentation when detecting that the glucose content in the fermented liquid is 1.3g / L, obtain 10L L-valine fermented liquid;

[0046] (2) Sterilizing the L-valine fermentation liquid at 0.23 MPa and 53° C. for 25 minutes to prepare the L-valine fermentation sterilization liquid;

[0047] (3) The L-valine fermented and sterilized solution was filtered through ceramic membrane and nanofiltration membrane in turn to obtain 13L of filtrated liquid; wherein the pressure of the ceramic membrane into the membrane was 0.3MPa, and the flux was 6L / min / m 2 , the material temperature is 35°C; the molecular weight cut-off of the nanofiltration membrane is 2000D, the membrane inlet pressure is 0.6MPa, and the flux is 0.6L / m...

Embodiment 2

[0050] (1) Inoculate the seed culture solution into 10L fermentation medium according to the inoculum size of 2%, ferment and cultivate at 35°C and pH6-7 until the OD value of the solution is 30, add 0.15g of sterilized isoascorbic acid, and continue Cultivate, stop fermentation when detecting that the glucose content in the fermented liquid is 1.3g / L, obtain 10L L-valine fermented liquid;

[0051] (2) Sterilizing the L-valine fermentation broth at 0.23MPa and 53°C for 25 minutes to prepare the L-valine fermentation sterilization fluid;

[0052] (3) The L-valine fermented and sterilized solution is filtered with ceramic membrane and nanofiltration membrane in turn to obtain 13L of filtrated liquid; wherein the membrane inlet pressure of the ceramic membrane is 0.3MPa, and the flux is 6L / min / m 2 , the material temperature is 35°C; the molecular weight cut-off of the nanofiltration membrane is 2000D, the membrane inlet pressure is 0.6MPa, and the flux is 0.6L / min / m2 , the materi...

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Abstract

The invention relates to an efficient decoloring method of amino acid fermentation liquor, which specifically comprises the following steps: (1) inoculating amino acid producing bacteria into a fermentation culture medium, conducting fermenting and culturing until the OD (optical density) value is greater than or equal to 3, and adding an antioxidant until the fermentation is finished to obtain a fermentation liquor; and (2) decolorizing the fermentation liquor with activated carbon, and conducting filtering to obtain a decolorized clear solution. According to the method, the antioxidant is added into the solution in the fermentation culture state, so that the generation of pigment and pigment precursor substances can be inhibited or reduced, the difficulty of subsequent activated carbon decoloration is reduced, and the overall decoloration efficiency of the fermentation liquor is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an efficient decolorization method for amino acid fermentation liquid. Background technique [0002] The preparation of amino acids by microbial fermentation method uses glucose as raw material, and is fermented by microorganisms to obtain amino acid fermentation liquid. The amino acid fermentation broth contains pigment impurities, which are mainly the pigments brought by the raw materials of the amino acid fermentation broth, the pigments produced by microbial metabolism and carbonyl ammonia reaction during fermentation, and the precursor substances produced during the fermentation process, which are further transformed during heat treatment such as recrystallization. of pigment. If these pigments are not removed, the color of the fermentation broth will be dark, which will affect the quality of the product. [0003] In the prior art, the pigment content is mainly reduced by deco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08B01D15/08C12N1/20C12R1/13
CPCC12P13/08C12N1/20B01D15/08
Inventor 余军刘树蓬刘磊
Owner ANHUI HUAHENG BIOTECH
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