Culture box for plant tissue culture

Abstract

The invention relates to the technical field of plant breeding, and discloses a culture box for plant tissue culture. The box comprises: an box body; a plug block is arranged at the lower end of the box body; a connecting frame is arranged in the box body, wherein the outer surface of the connecting frame is fixedly connected with the inner wall of the box body, a collecting mechanism is arranged at the lower end of the connecting frame, a connecting ring is arranged at the lower end of the collecting mechanism, and a deflection mechanism is arranged in the connecting ring; a fixing ring is fixedly connected to the top of the inner wall of the box body; a drainage pipe is connected to the center axis of the bottom of the box body, wherein a plug block is in plug connection with the drainage pipe. By arranging an annular plate and a vibrating membrane, root seedling metabolites in the box body can move downwards along the annular plate and the vibrating membrane along with descending of a nutrient solution until the root seedling metabolites fall into the fixing ring and then are discharged through the drainage pipe so as to conveniently clean the root seedling metabolites. Thus, the root seedling metabolites are effectively prevented from being adhered to the surfaces of the annular plate and the vibrating membrane by making the nutrient solution fluctuate then to drive vertical rods and the vibrating membrane to vibrate.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to an incubator for plant tissue culture. Background technique [0002] The concept of plant tissue culture (in a broad sense) is also called in vitro culture, which refers to the separation of tissues that meet the needs from the plant body. Organs or cells, protoplasts, etc., are cultured under artificially controlled conditions to obtain regenerated whole plants or produce other products of economic value through aseptic manipulation. The concept of plant tissue culture (narrow sense) refers to the cultivation of various parts of plants, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants, and also refers to the cultivation of callus produced from various organs during the cultivation process. The callus is then redifferentiated to form a regenerated plant. [0003] Plant tissues are cultured in the culture medium, but during the g...

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Problems solved by technology

[0003] Plant tissues are cultured in the culture medium, but during the growth and development of plant roots, root metabolites are easy to accumulate in the culture medium, and it is not convenient to clean up the root metabolites adhering to the box when changing the culture medium, so we propose incubator for plant tissue culture

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