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Process for producing thymidine by fermentation method

A fermentation method and technology of thymidine, applied in the field of β-thymidine preparation, can solve the problems of increasing production cost, low production efficiency, low utilization rate of culture medium, etc., so as to improve utilization rate, reduce processing cost and enhance practicability. Effect

Pending Publication Date: 2022-02-11
JIANGXI CHENGZHI BIOENG
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  • Claims
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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a process for producing thymidine by fermentation, which solves the problem that the utilization rate of the culture medium in the process of producing thymidine by the existing biological fermentation method is low, the production cost is increased, and the production efficiency is not high. question

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  • Process for producing thymidine by fermentation method

Examples

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Embodiment 1

[0033] Such as figure 1 As shown, the embodiment of the present invention provides a process for producing thymidine by fermentation, including inoculation, screening, amplification, fermentation, extraction and purification. The specific process flow is:

[0034] S1. Vaccination

[0035] In a sterile environment, spread and inoculate the culture medium with Escherichia coli strains that have been implanted with relevant genes, put them into the culture room, and carry out colony cultivation. The culture dish is GYT medium, and the colony cultivation time is 24 hours;

[0036] S2. Screening

[0037] After the colony cultivation is completed, in a sterile environment, screen and cut off the colonies that reproduce faster, make a dispersion, and take 2ml of the dispersion for 1000-fold dilution, and detect the number of E. coli in the unit volume of the dispersion;

[0038] S3. Amplification

[0039] The prepared dispersion liquid is evenly mixed into the liquid medium at a r...

Embodiment 2

[0047] The difference between this example and Example 1 is that the production process of thymidine by fermentation method includes inoculation, screening, amplification, fermentation, extraction and purification, and the specific process flow is as follows:

[0048] S1. Vaccination

[0049] In a sterile environment, spread and inoculate the culture medium with Escherichia coli strains implanted with relevant genes, put them into the culture room, and carry out colony cultivation. The culture dish is GYT medium, and the colony cultivation time is 36 hours;

[0050] S2. Screening

[0051] After the colony cultivation is completed, in a sterile environment, screen and cut off the colonies that reproduce faster, make a dispersion, and take 2ml of the dispersion for 1000-fold dilution, and detect the number of E. coli in the unit volume of the dispersion;

[0052] S3. Amplification

[0053] Mix the prepared dispersion evenly into the liquid medium at a ratio of 1:100, put it in...

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Abstract

The invention provides a process for producing thymidine by a fermentation method, and relates to the technical field of beta-thymidine preparation. The process for producing thymidine by the fermentation method comprises the steps of inoculation, screening, amplification, fermentation, extraction and purification. By means of secondary culture amplification processing, a large number of strain bags convenient to quantitatively and directly use are prepared through gel films, and when initial fermentation, tank body maintenance and fermentation nutrient solution replacement are carried out, direct proportioning and use are facilitated, the working efficiency is improved, residual solution obtained after filtration flows back to a fermentation tank through circulation equipment. The nutrient substances left in the residual solution can be reused, so that the utilization rate of the nutrient solution is increased, the processing cost is reduced, and the practicability of the nutrient solution is enhanced.

Description

technical field [0001] The invention relates to the technical field of beta-thymidine preparation, in particular to a process for producing thymidine by a fermentation method. Background technique [0002] β-thymidine, also known as 2'-deoxythymidine, is the precursor of anti-AIDS stavudine (3'-deoxy-2', 3'-didehydrothymidine) and zidovudine. At present, there are three main methods for the production of thymidine. One is the production of thymidine by chemical synthesis, the process is very lengthy, and the formation of glycosidic bonds lacks stereospecificity, making the cost of the final thymidine relatively expensive. The second is the biological enzymatic method, which has the advantage of strong specificity, but the cycle is long and the product is not easy to separate. The third is the biological fermentation method, which has a simple process and low subsequent separation costs, and is a very promising production method. Therefore, the research on the method of ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/38C12N1/21C12R1/19
CPCC12P19/385C12N1/20
Inventor 贾伟李聪发王水淋张勇辉赵磊叶婷
Owner JIANGXI CHENGZHI BIOENG
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