DNA nanostructure, construction method and application

Abstract

The invention belongs to the technical field of nanostructures, and discloses a DNA nanostructure, a construction method and application, and the DNA nanostructure is formed by using RNA and modified RNA through a folding support DNA chain. According to the invention, a short RNA chain is used as a clip to guide the formation of a DNA nanostructure; the DNA nanostructure is formed by folding a stent DNA chain with RNA and modified RNA (2'-Z-RNA), the construction method of the DNA nanostructure is enriched, and compared with a DNA nanostructure obtained by a conventional DNA origami technology, the folding efficiency is higher, the purity of the DNA nanostructure obtained by RNA folding is higher, and a good foundation is provided for wide application of the DNA nanostructure. According to the invention, the DNA scaffold chain is efficiently assembled into a designed shape and structure by using the short RNA clip, and an architectural platform is provided for folding various complex DNA nano materials by using a strategy of folding DNA by using the RNA clip.

Description

technical field [0001] The invention belongs to the technical field of nanostructures, and in particular relates to a DNA nanostructure, a construction method and an application. Background technique [0002] At present, RNA self-assembly is widespread, such as RNA quadrilateral, tetrahedron, k-turn, pRNA-3WJ, viral IRES models, because of their high diversity, long RNA chains are difficult to predict. In contrast, strands of DNA are programmable and can be folded manually. DNA is undoubtedly the most promising self-assembling molecule based on Watson-Crick base pairing. DNA can self-assemble into 2D and 3D nanostructures (such as DNA triangles, DNA tetrahedra, and other rationally designed recombinant DNA geometries), making them potentially useful in a variety of applications. A long DNA scaffold can be folded into different nanostructures, DNA origami, by strands of DNA staples. With the help of computing, people discovered DNA origami technology, and DNA nanostructure...

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the existing problems and defects of the prior art are: due to the relatively high cost of RNA and the need to introduce a piece of DNA to participate in the pairing, the assembly efficiency of preparing larger DNA nanostructures is low

[0005] The difficulty in solving the above problems and defects is: large DNA nanostructures require longer DNA and RNA strands, and the cost of preparing longer DNA and RNA single strands is relatively high and the purity is relatively low, and they are more easily degraded by nucleases. Moreover, longer nucleic acid chains are more prone to base mispairing, which further restricts the assembly efficiency of large DNA nanostructures.

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