Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor

An electrochemical and sensor technology, which is applied in the field of electrochemical sensors for rapid and sensitive determination of tetracycline and its preparation, can solve the problems of expensive instruments, time-consuming and specialized technicians, complex sample derivation processing steps, etc., to improve detection sensitivity and facilitate storage , Accelerate the effect of electron transfer

Active Publication Date: 2022-02-18
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods usually involve disadvantages such as expensive instruments,

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  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor

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[0035] As described in the context, tetracycline will have adverse effects on our human body, and it is necessary to further increase the detection selectivity and sensitivity of tetracycline. Based on this, the present invention provides a rapid sensitivity to measure the electrochemical sensor of tetracycline and its preparation method and application thereof. The graphene nanofine in the present invention is a nano-belt made of multi-walled carbon nanotubes, and then binds to a mercapto group and a gold nanoparticle, and then supported on a graphene nano.

[0036] Glosene nano-with a two-dimensional crystal carbon nanomaterial due to its well-conductive and electrochemical stability, has a unique advantage in electrochemical sensing detection. However, the pure graphene nano-belt is not easy to solve with water, resulting in a certain limitation in the application. The cyclodextron has a special structure of "exulse water, internal hydrophobic", and can indirectly promote "diss...

Example Embodiment

[0038] Example 1: Preparation of Electrochemical Sensor

[0039] (1) Preparation of the oxide oxide nanoji. Add 0.1g MWCNT to 150mlh 2 SO 4 / H 3 PO 4 (9: 1) of the flask, stirred for 1 hour to add 0.5 g kmno 4 The mixture was stirred at 65 ° C for 2 hours, and 0.5mlh was added after ice bath. 2 O 2 Finally, the solution was filtered with 0.45 μm filtration, and washed multiple times with deionized water to be neutral, and then dried at room temperature for 24 hours.

[0040] (2) Preparation of gold nanoparticles / mercaptogencyclodextrin / graphene nanoflast. The oxide oxide nanocarbons prepared by step (1) were dissolved in the water (power 360 W, frequency 40 kHz) for 24 hours to disperse the graphemethylene nano-tape solution, and 20 mg was added to 40 ml of water to stir 0.5 mg ml. -1 Mercaptogencyclo cyclodextrin solution. 40 ml of oxide nano-nanocarbon solution (0.5 mg ml -1 ) And 40ml (0.5 mg ml -1 The SH-β-CD solution was stirred after mixing for 0.5 hours, then 1.2 ml (1...

Example Embodiment

[0043] Example 2:

[0044] Electrochemical detection of tetracyclines for AUNPS / SH-β-CD-GnRS / GCE prepared in Example 1

[0045] (1) In the potential range of 0.30 to 1.10 V, in the phosphate buffer solution of pH 6.5, a circulating voltammetry, SH-β-CD-GNRS / GCE and AUNPS / SH-β prepared in Example 1- CD-GNRS / GCE has a significant catalytic effect on tetracycline, as shown figure 1 and figure 2 As shown, in comparison, the AUNPS / SH-β-CD-GNRS / GCE prepared by Example 1 is better than the catalytic effect of tetracycline.

[0046] (2) In the potential range of 0.30 to 1.10 V, in the phosphate buffer solution of pH 6.5, the enriched time is 250 s, the enriched potential is 0.30V, and the scanning rate of circulating voltammetry (5mV / s, 10mv / S, 20MV / S, 40MV / S, 60M V / S, 80MV / S, 100 mV / s) obtain a series of CV curves. As the scan rate increases, the peak current of the two oxidation peaks has gradually increased. And the peak current and scan rate of the two peak...

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Abstract

The invention discloses an electrochemical sensor for measuring tetracycline as well as a preparation method and application thereof. The preparation method comprises the steps that a graphene oxide nanobelt is prepared firstly, then a thiolated cyclodextrin/graphene oxide nanobelt solution is prepared, then a HAuCl4 solution is added, an AuNPs/SH-beta-CD-GNRs composite material is prepared by means of the strong reducibility of hydrazine hydrate and the S-Au effect. On the basis, an AuNPs/SH-beta-CD-GNRs/GCE electrode is constructed. As a good electric conductor, the prepared composite material can effectively accelerate electron transfer between an electrode and a solution, so that the detection sensitivity is improved. Under the optimal condition, tetracycline is detected by using linear scanning voltammetry, the linear range is 0.05-240 microns, the detection limit is 0.016 micron (S/N = 3), and good repeatability and selectivity are achieved.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for rapid and sensitive determination of tetracycline and its preparation method and application. Background technique [0002] Tetracycline is the most common antibiotic used to treat bacterial infections. It can be used for things like urinary tract infections, chlamydia, and acne-like acne on the skin. However, its improper abuse will cause residues in animal-derived foods (such as meat, honey, and chicken, etc.), which will cause harm to human body drug resistance, vision, teeth, and allergies. The Ministry of Agriculture of my country has established corresponding maximum residue limit standards for tetracycline antibiotics that have been approved for use (for example, tetracycline in pork and beef is not higher than 100 μg / kg). Therefore, it is of great significance to develop a sensitive, high-selection and rapid detection method...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 汪小强邓昌晞李铭芳伍忠汉
Owner JIANGXI AGRICULTURAL UNIVERSITY
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