Production method of immune electrode

A technology of electrodes and conductive polymers, which is applied in the field of preparation of immune electrodes, can solve the problems that the specificity of antibody-antigen reactions needs to be enhanced, and the stability of electrode modification materials needs to be improved, so as to facilitate a large number of detections, improve detection efficiency and accuracy, The effect of improving sensitivity

Pending Publication Date: 2021-06-18
NANJING LANSION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among them, the electrode is the core of the electrochemical sensor, and nanomaterials or composite materials can be used to prepare modified electrodes, so that the electrodes have more functions, so that they are suitable for a variety of experimental requirements and detection requirements; these materials mainly include: Graphene, graphene-gold nanoparticles, graph

Method used

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  • Production method of immune electrode
  • Production method of immune electrode
  • Production method of immune electrode

Examples

Experimental program
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Example Embodiment

[0067] Example 1: Such as Figure 9 As shown, the immune electrode includes a base layer 10, a gold layer 11, a conductive polymer layer 12, an antibody layer 13, and a porous protective layer 14, the base layer 10, a gold layer 11, a conductive polymer layer 12, an antibody layer 13, and aperture protection. The layer 14 is bonded from the bottom to top, the base layer 10 being a PET flexible base layer; the conductive polymer layer 12 is a polypyrrole.

Example Embodiment

[0068] Example 2: Preparation method of the polypyrrole immunoelectric electrode, including the step of: (1) synthesizing polypyrrole layer (conductive polymer layer): prepared polypyrrole layer after gold plating base layer, obtained Pyrrole / gold-plated base;

[0069] S11 uses ethanol, ultrapure water, ultrasound, dry and ready for drying.

[0070] S12 is fixed to the bottom of the 1000 ml beaker, then adding the reaction liquid to the beaker, including pure water, hydrochloric acid, ferricide and pyrrole; first to the beaker to add 400 ml of pure water and 6.4 Ml1mol / L hydrochloric acid, stirred and uniformly added 0.88 g of trichloride, and then stir evenly, place the beaker under ice bath, and slowly add 400 μl of pyrrole under the nitrogen atmosphere, and then lighted under the ice bathing nitrogen atmosphere. The reaction is reacted, and the time to react overnight is 10 to 16 h; after repeated washing with pure water, drying under a nitrogen atmosphere, a layer of black...

Example Embodiment

[0080] Example 3: A different from Example 2 is in that the conductive polymer layer in the immunoelectrode is a polypyrrol doped with sodium dodecyl sulfonate; the preparation method of polypyrrole immune electrode, Include the following steps:

[0081] (1) Preparation of a conductive polymer layer: prepared a polypyrrole layer on a gold-plated base layer to obtain a polypyrrole / gold-plated base layer;

[0082] S11 uses ethanol, ultrapure water, ultrasound, dry and ready for drying.

[0083] S12 secures the gold plated PET base layer of the dry-drying to the bottom of the 1000 ml beaker, then adding the reaction liquid to the beaker, the reaction liquid comprising pure water, hydrochloric acid, ferricide, pyrrole and sodium dodecyl sulfonate (SDS) In first, 400 ml of pure water and 6.4 ml of 1 mol / L hydrochloric acid were added to the beaker. After stirring, 0.88 g of ferricide and 0.17 g of sodium dodecyl sulfonate (SDS) were slowly added, and then stirred even after mixing ...

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Abstract

The invention discloses a production method of an immune electrode. The immune electrode comprises a base layer, a gold layer, a conductive polymer layer and an antibody layer, and the base layer, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The production method of the immune electrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated base layer to obtain a polypyrrole/gold-plated base layer; (2) producing the immune electrode: preparing an antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated base layer; and (3) forming an immune electrode system: fixing the bare gold-plated base layer and the outer side of the antibody/polypyrrole/gold-plated base layer to obtain the immune electrode system. The polypyrrole material is used for fixing a biological recognition element antibody, the biological recognition element antibody is immobilized on an immune electrode, and polypyrrole has excellent conductivity so that electrode surface electron transfer can be accelerated, and the sensitivity of the immune electrode is improved.

Description

technical field [0001] The invention belongs to the field of electrochemical immunodetection, in particular to a preparation method of an immunoelectrode. Background technique [0002] Electrochemical immunosensor is an analytical method based on the specific combination of antigen and antibody; it has the advantages of rapid detection, low detection limit, high sensitivity, simple operation and low preparation cost. [0003] In recent years, electrochemical immunosensors have attracted much attention and have been widely used in the detection of tumor markers. When immunosensors are used for continuous target detection, the reproducibility of biomolecular probes is a major problem restricting the practical application of this type of sensors. Therefore, the development of immunosensors that can be mass-produced and low-cost, and used as disposable sensors, will overcome the disadvantages of cumbersome operations, time-consuming and labor-intensive operations caused by the ...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N27/327
CPCG01N33/531G01N27/3277G01N27/3278G01N33/5438G01N27/3275B01L3/502707B01L2300/0645B01L2300/0816B01L2300/087G01N27/3276G01N33/547
Inventor 许行尚杰弗瑞·陈
Owner NANJING LANSION BIOTECH CO LTD
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