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Cadmium ion microfluidic detection method and detection device based on nano-particle enzyme-linked sensibilization

A technology of nanoparticles and detection methods, applied in measurement devices, chemical instruments and methods, testing water, etc., can solve the problems of high cost, long time, complicated operation, etc., and achieve the effect of convenient detection and improved reliability.

Pending Publication Date: 2022-02-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The World Health Organization stipulates that the limit standard for cadmium ions in drinking water is 3 μg / L. However, many detection methods developed so far have problems such as time-consuming, high cost, and complicated operation. Therefore, it is urgent to establish a low-cost rapid detection of cadmium. ionic method

Method used

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  • Cadmium ion microfluidic detection method and detection device based on nano-particle enzyme-linked sensibilization
  • Cadmium ion microfluidic detection method and detection device based on nano-particle enzyme-linked sensibilization
  • Cadmium ion microfluidic detection method and detection device based on nano-particle enzyme-linked sensibilization

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Embodiment 1

[0068] refer to Figure 3 to Figure 10 , is the first embodiment of the present invention, which provides a microfluidic detection method for cadmium ions based on nanoparticle enzyme-linked sensitization, which is convenient for detection and can realize the detection of heavy metals in fresh water.

[0069] A cadmium ion microfluidic detection method based on nanoparticle enzyme-linked sensitization, which comprises the following steps:

[0070] Inject the water sample to be tested into the storage cavity 206a of the water sample to be tested in the detection chip;

[0071] The chelating agent storage chamber 206b in the detection chip and the water sample storage chamber 206a to be tested respectively output the chelating agent and the water sample to be tested at the same time, and the water sample to be tested and the chelating agent mix and react to form chelates, and the chelates enter the detection chip. Reaction chamber 206w;

[0072] The AChE-AuNPs-antibody solutio...

Embodiment 2

[0084] As the second embodiment of the present invention, the difference from the first embodiment is that this embodiment provides a microfluidic detection method for cadmium ions based on nanoparticle enzyme-linked sensitization, which includes the following steps:

[0085] Open the liquid inlet sealing cover 204, drop the water sample to be tested into the liquid inlet tank 207d, close the liquid inlet sealing cover 204 and let stand for 1 min;

[0086] Press the first pressing column 203 and the second pressing column 202, the water sample to be tested and the chelating agent are mixed and reacted to form a chelate. 18.5 μL;

[0087] Press the first reaction pressing column 213 to the bottom 5-6 times, the AChE-AuNPs-antibody solution entering the reaction pool is 77.1-80.2 μL, release the first reaction pressing column 213;

[0088] Press the second reaction pressing column 212 to the bottom 3-4 times, the Fe3O4-antigen solution entering the reaction pool is 22.2-22.9 μL...

Embodiment 3

[0094] For the third embodiment of the present invention, the difference with the 1st and 2nd embodiment is that this embodiment provides the method for preparing chelating agent, and it may further comprise the steps:

[0095] Pour 10mmol / L ethylenediaminetetraacetic acid solution into 0.01mol / L HEPES buffer solution with pH 7.4 at a ratio of 1:10, mix well, and use it as a chelating agent for free cadmium ions.

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Abstract

The invention discloses a cadmium ion microfluidic detection method based on nano-particle enzyme-linked sensibilization. The method comprises the following steps that: a to-be-detected water sample is injected into a to-be-detected water sample storage cavity in a detection chip; the to-be-detected water sample and a chelating agent are mixed and react to generate a chelate, and the chelate enters a reaction cavity of the detection chip; an AChE-AuNPs-antibody solution and a Fe3O4-antigen which are stored in the detection chip are sequentially extruded into the reaction cavity to react with the chelate; after standing for 2 minutes and magnetic separation, the clear water stored in the detection chip is squeezed into the reaction cavity, the floating clear liquid is washed away, and the floating clear liquid enters a waste liquid cavity; an Ach solution stored in the detection chip is squeezed into the reaction cavity, so that the reaction tank is filled with the Ach solution, and a large amount of mixed liquid enters the waste liquid cavity; and a detector detects the voltage of an electrode assembly inserted into the reaction cavity, and obtains the relation between the voltage and the ph value and the relation between the ph change and the cadmium ion concentration. The method is convenient in detection and high in detection efficiency.

Description

technical field [0001] The invention relates to the technical field of fresh water detection, in particular to a device for automatically detecting the quality of fresh water and a method for detecting the quality of fresh water. Background technique [0002] Heavy metal pollution has always been a problem of widespread concern. As a common heavy metal, cadmium is widely used in industrial fields such as nickel-cadmium batteries, paint coloring and electroplating. Cadmium is a toxin that can be enriched through the food chain and ingested in water. The accumulation of cadmium in human organs such as the lungs, kidneys and liver can cause damage to the kidneys, liver, cardiovascular and nerves. Cadmium pollution is a serious threat to human health and has been listed as one of the major public hazards of environmental and food pollution. The World Health Organization stipulates that the limit standard for cadmium ions in drinking water is 3 μg / L. However, many detection met...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N27/00G01N35/00B01L3/00
CPCG01N33/18G01N27/00G01N35/00B01L3/5027
Inventor 尹彬沣周佟杨超
Owner YANGZHOU UNIV