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A detection method for evaluating soil activity

A detection method and soil technology, applied in biochemical equipment and methods, microbial measurement/inspection, instruments, etc., can solve problems such as inability to accurately reflect soil vitality, achieve low time cost, reduce workload, and improve the operation process simple effect

Active Publication Date: 2022-07-19
宁波市农业技术推广总站
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AI Technical Summary

Problems solved by technology

Since the traditional plate culture method can only reflect the information of a very small number of microorganisms, it cannot accurately reflect the vitality of the soil

Method used

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  • A detection method for evaluating soil activity

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Embodiment 1

[0023] like figure 1 As shown, a detection method for evaluating soil activity includes a library acquisition step, a functional gene identification step, a carbon element monitoring step, a nitrogen element monitoring step and an activity analysis step,

[0024] In the library acquisition step, the soil to be detected is acquired, the DNA of the microorganisms in the soil to be detected is obtained by soil metagenomics technology, the extracted microbial DNA is detected and a DNA library is constructed, and the functional gene identification step is entered;

[0025] In the functional gene identification step, the coding gene obtained by the microorganism participating in the nitrogen cycle reaction is recorded as a nitrogen cycle reference gene, and the coding gene obtained by the microorganism participating in the carbon cycle reaction is recorded as a carbon cycle reference gene, and the nitrogen cycle reference gene and the carbon cycle reference gene are obtained. The ci...

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Abstract

The invention discloses a detection method for evaluating soil activity. The soil metagene technology is used to analyze the overall DNA of microorganisms in the soil and a DNA library is constructed, and the microarray technology is used to identify the DNA sequences in the DNA library in batches to quickly determine The abundance of coding genes involved in carbon cycle and nitrogen cycle in the extracted soil microbial DNA, so as to obtain the frame diagram of carbon cycle and nitrogen cycle species involved in the tested soil microorganism, and then determine the soil microorganism by measuring the amount of carbon dioxide in the soil. The total change in respiratory carbon, by using 15 The N radiolabeled induction substrate induces the expression of the DNA of the soil microorganisms to determine the amount of biological nitrogen change of the soil microorganisms, thereby determining the total amount of soil participating in carbon and nitrogen cycles and obtaining soil activity information. The whole operation process is simple, the time-consuming cost is very low, and it is close to the real overall soil activity.

Description

technical field [0001] The invention relates to the technical field of soil activity detection, in particular to a detection method for evaluating soil activity. Background technique [0002] Soil vigor is an important prerequisite for the good growth of crops. In the soil, there are a huge number of bacteria, fungi, actinomycetes, algae and other microorganisms, and their individuals are small. They carry out biochemical reactions such as oxidation, nitrification, ammoniation, nitrogen fixation, and sulfide in the soil to promote the decomposition of soil organic matter. and nutrient transformation, and play a role in soil occurrence, development and soil fertility. The species, quantity and activity of soil microorganisms reflect the intensity of soil biochemical activities, and naturally become important indicators for evaluating the degree of soil activity. [0003] Most of the traditional analysis of microbial community diversity and structure in soil ecosystems is to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/30G16B50/00C40B50/06C40B20/00C12Q1/6809
CPCG16B20/30G16B50/00C12Q1/6809C40B50/06C40B20/00C12Q2565/50C12Q2563/101
Inventor 王旭伟
Owner 宁波市农业技术推广总站
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