Construction method and application of animal model with chronic venous thrombosis induced by ovarian cancer
A venous thrombosis, animal model technology, applied in veterinary instruments, veterinary surgery, medical science, etc., can solve the problems of vascular endothelial injury, not suitable for simulating tumor thrombosis, etc.
Inactive Publication Date: 2022-03-01
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology
Although this method is suitable as a dynamic animal model, it also causes damage to the vascular endothelium when the blood vessel is ligated, and is still not suitable for simulating tumor thrombosis
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Embodiment 1
[0026] as per figure 1 The method shown is to construct an animal model of chronic venous thrombosis induced by ovarian cancer, and the steps are as follows:
[0027] Step 1: Cell culture and reagent screening were carried out first to obtain the human ovarian serous adenocarcinoma cell line SKOV3. All cells were used in RPMI-1640 medium (Shanghai Senbu Biotechnology) containing 10% fetal bovine serum (Shanghai Kechuan Biotechnology) , 2mM glutathione (Shanghai Kechuan Biology) and 100U / ml penicillin (Shanghai Zhaoqi Biotechnology Co., Ltd.), the cell culture environment is 37 ℃, 95% air-5% CO 2 Humidified incubator. Harvest the SKOV3 cells according to 1×10 7 The number of intraperitoneal injections into each SCID mouse to establish intraperitoneal xenograft tumor SCID mice, the formation of abdominal tumors and the survival rate of SCID mice were evaluated on the 14th day from the day of injection of cancer cells until there were 8– 10 tumor foci were formed, and the tumo...
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The invention discloses a construction method and application of an ovarian cancer-induced chronic venous thrombosis animal model, and the construction method comprises the following steps: injecting an SKOV3 ovarian cancer cell line into the abdominal cavity of an SCID mouse according to the dosage of 1 * 10 < 7 > cells / 200 [mu] LPBS for each mouse, and when 8-10 tumor focuses are formed in the mouse body and the tumor is not enough to enable the mouse to die in a short period of time, constructing an SKOV3 ovarian cancer cell line; the method comprises the following steps: selecting 20-80% of inferior vena cava stenosis degree to carry out venous stenosis operation treatment on a mouse, and carrying out inferior vena cava ultrasonic examination and inferior vena cava sampling HE dyeing after operation until thrombus is formed. Thrombus is formed through a venous stenosis method on the basis of an ovarian cancer abdominal transplantation tumor animal model, modeling is stable, safe and operable, the biological behavior that ovarian cancer induces chronic venous thrombosis is met, and key molecules for promoting ovarian cancer to generate VTE and downstream regulation and control targeted secretory protein of the key molecules can be found.
Description
technical field [0001] The invention relates to the technical field of animal models, in particular to a method for constructing an animal model of ovarian cancer-induced chronic venous thrombosis and its application. Background technique [0002] Venous thromboembolism (VTE), as a common complication in patients with malignant tumors, is second only to the cause of death caused by the tumor itself, but how tumor-related VTE occurs remains to be further explored. Animal models of VTE are important modeling tools for understanding the mechanism of VTE. [0003] According to different purposes, there are great differences in VTE animal models in terms of animal type, thrombus production and evaluation indicators. The formation of thrombus is the key to the establishment of thrombus animal models, mainly including in vitro thrombus formation and in vivo thrombus formation. In vitro thrombus formation is to draw animal venous blood under sterile conditions, and form thrombus by...
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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 臧荣余史庭燕郑义艳尹胜马思宁
Owner ZHONGSHAN HOSPITAL FUDAN UNIV