Group of ginkgo gender-associated SNP sites and primers and application thereof

Abstract

The invention discloses a group of ginkgo gender-associated SNP sites as well as primers and application thereof, and belongs to the technical field of plant gender identification. According to the method, 160 samples from 11 gingko ancient tree populations are subjected to SNP site mining and genetic typing through a simplified genome technology, SNP sites are filtered through GQ, MQ, minimum allele frequency, Miss rate, reads number parameters and Hardy-Weinberg genetic balance conditions, and a mixed linear model is adopted to perform whole genome association analysis on gingko sex. Screening according to the positions of the SNP loci and genetic typing information to obtain six SNP loci, namely GbSNP 1 to GbSNP 6, associated with the gender of the ginkgo biloba; the primers for detecting the six SNP loci are correspondingly designed, and the SNP loci or the primers are used for early sex identification of the gingko species level, so that the method is simple, convenient and rapid, and the result is accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of plant sex identification, and in particular relates to a group of ginkgo sex-associated SNP sites, primers and their application in early sex identification of ginkgo species. Background technique [0002] Ginkgo biloba L. is a unique relic plant in my country, known as the "living fossil" of the plant kingdom. As a typical dioecious plant, there are obvious differences between the female and male plants in terms of morphological characteristics, growth habits and application value. Ginkgo male plants grow fast, have beautiful tree shape and strong resistance, and are often used for landscaping [Tang Haixia, Du Shuhui, Xing Shiyan, Sang Yalin, Li Jihong, Liu Xiaojing, Sun Limin. Screening of Ginkgo Sex Determination Related Genes[J] . Forestry Science, 2017, 53(2): 76-82.]. Ginkgo seeds have important edible value and medicinal value, so the female plant is often used for fruit cultivation. However, af...

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Problems solved by technology

However, generally speaking, it takes 20-30 years for ginkgo seedlings to bloom, and then the gender will be directly identified, which lacks timeliness and is a great limitation in popularization, application and scientific research practice.

Although there are scholars from the morphology [Huang Fengli. Ginkgo tree male and female plant morphological identification method [J]. Shanxi Fruit Tree, 2009, (3): 56.], physiological and biochemical [Yamasaki M. Identification of the sexes in dioecious plants by testing the resistance to the toxic action of chloride[J]. Journal of Japanese Botany, 1933, 6: 459-466.; Cai Ru, Tao Jun, Chen Peng. Comparative study on photosynthetic characteristics, transpiration rate and yield of male and female ginkgo leaves[J]. Deciduous Fruit Trees, 2000, (1): 14-15.] and cytological level [Zhang Yang, Zhu Shengnan, Jin Xiaofen, Hong Yihuan, Chen Jianmin. 45SrDNA-FISH identification of male and female ginkgo [J]. Horticultural Science, 2007, (6): 1520 -1524.] et al. have explored the early sex identification method of ginkgo, but it is still affected by factors such as environment, tree age, and place of origin, and has not been able to fundamentally solve this problem.

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