Cancer vaccine composition and method for preventing and/or treating cancer by using same
A cancer vaccine, cancer cell technology, applied in breast cancer vaccines, vaccines, cancer antigen components, etc.
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example 1
[0975] Example 1: Materials and methods for Examples 2-7
[0976] a.Cell culture
[0977] After adding 10% fetal bovine serum (FBS), 25ng / ml hydrocortisone, 5μg / ml insulin, 8.5ng / ml cholera toxin, 0.125ng / ml epidermal growth factor (EGF), 5μM Y-27632Rock1 inhibitor, penicillin PP and PP T breast cancer cells. for PP T cells, new 4ng / ml TGFβ1 was added to the medium every three days. at 37°C in 5% CO 2 Cells were grown in a humid environment. NMuMG, HMEC, MCF10A, ZR-75-1, MDA-MB-453, MDA-MB-231, MCF7, BT549, HCC1954, and HCC70 cells were purchased from the American Type Culture Collection (ATCC) and cultured according to the manufacturer's instructions .
[0978] b. Antibodies and reagents
[0979] TGFβ1 (#GF111) was purchased from Millipore (Billerica, MA, USA). FITC anti-mouse CD45(30-F11), PE / Dazzle were purchased from Biolegend (San Diego, CA, USA) TM 594 anti-mouse CD3(145-2C11), APC / Cy7 anti-mouse CD4(RM4-5), Alexa 700 anti-mouse CD8 (53-6.7), APC anti-mouse ...
example 2
[1002] Example 2: TGFβ-treated tumor cells induce T cell-dependent anti-tumor immune responses.
[1003] Transforming growth factor beta (TGFβ) is a pluripotent cytokine that plays a key role in regulating embryonic development, cell metabolism, tumor progression, and immune system homeostasis (David and Massague (2018), Nature Reviews Molecular Cell Biology, 19:419-435). After TGFβ binds to its receptor on the plasma membrane, it regulates the expression of its downstream genes in a Smad-dependent and independent manner ( Figure 16 ).
[1004] Loss of the tumor suppressors p53 or PTEN is the most common event in human cancers (Lawrence et al. (2014) Nature 505:495-501). Most advanced epithelial tumors, including triple-negative breast cancer (TNBC), lack p53 and PTEN (The Cancer Genome Atlas Network (2012), Nature, 490:61-70). A syngeneic genetically engineered mouse model (GEMM) of TNBC derived from female FVB mice (carrying K14-Cre; Trp53 L / L ; Pten L / L Concurrent abl...
example 3
[1006] Example 3: DCs play an important role in mediating TGFβ-induced anti-tumor immune responses.
[1007]Meanwhile, 6 days after transplantation, PP isolated from recipient mice and PP T Transcriptome analysis of a panel of 4604 cancer and immune-related genes on tumor tissue. Obviously, compared with PP tumors, PP T Expression of genes with Gene Ontology (GO) terms associated with activation of multiple immune pathways was significantly upregulated in tumors ( Figure 4A ). Genes encoding cytokines, cytokine receptors, and T cell co-stimulatory molecules were further confirmed to be significantly upregulated by real-time quantitative PCR ( Figure 4B ). Furthermore, compared with PP tumors, PP T Genes encoding class I and class II major histocompatibility complex (MHC) components, such as H2-D1, H2-Ab1, and Cd74, were significantly upregulated in tumor sites ( Figure 4B ). These data further confirm that PP T Cells are able to elicit robust immune responses in the...
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