Phosphatidyl-agaro-oligosaccharide and preparation method thereof
A technology of agar oligosaccharides and phosphatidyl, which is applied in the field of phosphatidyl glycosides, can solve problems such as environmental pollution, complicated operation, and uneven product composition, and achieve the effect of reducing production costs
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[0048] Example 1: PLD catalyzed enzymatic synthesis of phosphatidyl-agagar oligosaccharides
[0049] Proceed as follows:
[0050] (1) Dissolve 10 mg of phosphatidylcholine in 1 mL of cyclopentyl methyl ether to obtain an organic phase.
[0051] (2) Dissolve 0.12 g D-galactose in 1 mL citric acid-sodium citrate buffer (pH 6.0, 0.1 M) to obtain the aqueous phase.
[0052] (3) Mix the above organic phase and aqueous phase at a volume ratio of 1:1, add 1.4 U of PLD to form a two-phase reaction condition, and seal it in a brown vial.
[0053] (4) Put the brown vial in a constant temperature water bath, and react in the water bath at 40 °C and 200 r for 10 h.
[0054] (5) After the reaction, centrifuge (8000 r, 5 min) to obtain the supernatant, and obtain phosphatidyl-agagar oligosaccharides dissolved in organic solvents.
[0055] (6) After nitrogen blowing, a high concentration of phosphatidyl-agar oligosaccharides is obtained, and its structural formula is as follows: image 3...
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