Phosphatidyl-agaro-oligosaccharide and preparation method thereof

A technology of agar oligosaccharides and phosphatidyl, which is applied in the field of phosphatidyl glycosides, can solve problems such as environmental pollution, complicated operation, and uneven product composition, and achieve the effect of reducing production costs

Pending Publication Date: 2022-03-22
OCEAN UNIV OF CHINA
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many applications of chemical methods, but there are still some shortcomings, such as inhomogeneous product composition, complicated operation, and easy to cause environmental pollution, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Phosphatidyl-agaro-oligosaccharide and preparation method thereof
  • Phosphatidyl-agaro-oligosaccharide and preparation method thereof
  • Phosphatidyl-agaro-oligosaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: PLD catalyzed enzymatic synthesis of phosphatidyl-agagar oligosaccharides

[0049] Proceed as follows:

[0050] (1) Dissolve 10 mg of phosphatidylcholine in 1 mL of cyclopentyl methyl ether to obtain an organic phase.

[0051] (2) Dissolve 0.12 g D-galactose in 1 mL citric acid-sodium citrate buffer (pH 6.0, 0.1 M) to obtain the aqueous phase.

[0052] (3) Mix the above organic phase and aqueous phase at a volume ratio of 1:1, add 1.4 U of PLD to form a two-phase reaction condition, and seal it in a brown vial.

[0053] (4) Put the brown vial in a constant temperature water bath, and react in the water bath at 40 °C and 200 r for 10 h.

[0054] (5) After the reaction, centrifuge (8000 r, 5 min) to obtain the supernatant, and obtain phosphatidyl-agagar oligosaccharides dissolved in organic solvents.

[0055] (6) After nitrogen blowing, a high concentration of phosphatidyl-agar oligosaccharides is obtained, and its structural formula is as follows: image 3...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a phosphatidyl-agaro-oligosaccharide, which is prepared by the following method: phosphatidylcholine and agaro-oligosaccharide are catalyzed by phospholipase D in a two-phase reaction system to generate transphosphatidylation so as to synthesize the phosphatidyl-agaro-oligosaccharide, the agaro-oligosaccharide is selected from D-galactose and neoagarobiose; the phospholipase D is selected from PLDr34. The phosphatidyl-agaro-oligosaccharide disclosed by the invention is a novel phosphatidyl glycoside, phospholipid can be used for preparing a liposome material, and a transesterification product has an encapsulation function, so that the phosphatidyl-agaro-oligosaccharide disclosed by the invention has a good application prospect in the aspects of encapsulation and transportation, and can be used as an active substance to be applied to the aspects of liposome preparation, drug delivery and the like. According to the method disclosed by the invention, the generation conditions of the phosphatidylcholine and the agaro-oligosaccharide are explored, the novel phosphatidylglycoside is synthesized, reasonable guidance is provided for synthesis of other phosphatidylglycosides, and the method has a certain research prospect.

Description

technical field [0001] The invention relates to a phosphatidyl-agagar oligosaccharide and a preparation method thereof, belonging to the technical field of phosphatidyl glycosides. Background technique [0002] The ocean is a treasure trove and a potential source of numerous natural bioactive substances. In all tissues, phospholipids, as the basic substance of life, are composed of a hydrophilic head group and a hydrophobic tail. Base (PC) is most abundant in glycerophospholipids. [0003] Phospholipids can not only participate in the formation of cell membranes and maintain their biological functionality, but also play an important role in cell micelles and organelles. For example, some phospholipids are the precursors of various anti-inflammatory compounds, which can regulate systemic inflammation and have a positive effect on chronic diseases. Provide protection. Phospholipids can be modified under a variety of conditions, such as hydrogenation, acetylation, sulfonatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/02A61K47/26A61K9/127
CPCC12P19/12C12P19/02C12P7/6472C12P7/6481A61K47/26A61K9/127
Inventor 毛相朝张海洋吴虹艳孙建安贺晨曦
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap