Biotransformation and extraction method of ganoderic acids LTHA and LTCA
A technology of biotransformation and extraction methods, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as difficult separation of ganoderma acid, achieve good market economic value, and solve the effect of low abundance
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Embodiment 1
[0064] This embodiment provides a biotransformation and extraction method of ganoderma acid LTHA and LTCA, comprising the following steps:
[0065] (1) Biotransformation: configure liquid medium (composed of: peptone 5g / L, glucose 10g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, chloramphenicol 0.1g / L, and configure with water ; The pH of the medium is 6.2-6.6), put the medium into the shake flask, seal it with a sterile gas-permeable membrane, sterilize at 121°C for 20min, and cool to room temperature;
[0066] Take Ganoderma sessile bacteria and inoculate them into sterile medium, break up the hyphae with a sterilized electric tissue homogenizer, place them in a shaker at 28°C and 200r / min for 5 days for activation . Take the activated Sessile Ganoderma lucidum bacteria liquid, crush the mycelium with a sterilized electric tissue homogenizer, and insert it into a shaker flask with a 10% inoculation amount, and add dihydrotanshinone Ⅰ solution dissolved ...
Embodiment 2
[0075] Compared with Example 1, the difference of Example 2 is: 1. The cultivation temperature of biotransformation is set to 26°C; 2. The composition of the liquid medium is: peptone 4g / L, glucose 6g / L, dihydrogen phosphate Potassium 1g / L, magnesium sulfate 0.4g / L, chloramphenicol 0.05g / L, prepared with water.
Embodiment 3
[0077] Compared with embodiment 1, the difference of embodiment 3 is that:
[0078] Biotransformation: configure liquid culture medium (it is composed of: peptone 5g / L, glucose 10g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, chloramphenicol 0.1g / L, configure with water; culture Base pH is 6.2-6.6), put the culture medium into the shake flask, seal it with a sterile gas-permeable film, sterilize at 121°C for 20min, and cool to room temperature;
[0079] Take Ganoderma sessile bacteria and inoculate them into sterile medium, break up the hyphae with a sterilized electric tissue homogenizer, and culture them in a shaker at 28°C and 200r / min for 5 days. activation. Take the activated Sessile Ganoderma lucidum bacteria liquid, crush the mycelium with a sterilized electric tissue homogenizer, and insert it into a shaker flask with a 5% inoculation amount. After culturing for 48 hours, add dihydrotanshinone Ⅰ dissolved in ethanol Solution, so that the final co...
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