Sputum treatment method
A treatment method and sputum technology, applied in the field of fungal detection, can solve the problems of fungal spread, disease spread, throughput, etc., and achieve the effects of improving the detection rate, short time consumption, and easy to use
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Embodiment 1
[0025] A method for processing sputum, comprising the following steps:
[0026] S1: Prepare mixing kettle, centrifuge, oven and weighing instrument;
[0027] S2: Add trypsin, KOH, sodium chloride and ethanol into the mixing tank, mix and stir evenly, and make a treatment liquid;
[0028] S3: Add the fluorescent whitening agent, penetration enhancer, moisturizer and background counterstaining agent into the mixing tank, mix and stir evenly, and make a dyeing solution;
[0029] S4: Place the sputum samples in the treatment solution according to different proportions, and centrifuge them through a centrifuge;
[0030] S5: The centrifuged sample is dropped on a glass slide, dried by a dryer, then added with staining solution and covered with a broken piece, and observed with a microscope.
[0031] In the present embodiment, in the S2, a weighing instrument is used to quantify trypsin, KOH, sodium chloride and ethanol, 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodiu...
Embodiment 2
[0039] A method for processing sputum, comprising the following steps:
[0040] S1: Prepare mixing kettle, centrifuge, oven and weighing instrument;
[0041] S2: Add trypsin, KOH, sodium chloride and ethanol into the mixing tank, mix and stir evenly, and make a treatment liquid;
[0042] S3: Add the fluorescent whitening agent, penetration enhancer, moisturizer and background counterstaining agent into the mixing tank, mix and stir evenly, and make a dyeing solution;
[0043] S4: Place the sputum samples in the treatment solution in different proportions, and centrifuge them through a centrifuge,
[0044] S5: The centrifuged sample is dropped on a glass slide, dried by a dryer, then added with staining solution and covered with a broken piece, and observed with a microscope.
[0045] In the present embodiment, in the S2, a weighing instrument is used to quantify trypsin, KOH, sodium chloride and ethanol, 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodium chloride...
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