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Monitoring device for cell microdamage induction and bright field monitoring method

A monitoring device and cell technology are applied in the field of medical detection to achieve the effects of a reliable cell micro-damage induction method, simple equipment and simple structure

Pending Publication Date: 2022-04-08
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main technical problem to be solved in this application is: how to overcome the limitations of the existing cell micro-damage induction methods, and provide a new cell micro-damage induction method and a method for monitoring the degree of cell micro-damage based on bright field images

Method used

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  • Monitoring device for cell microdamage induction and bright field monitoring method
  • Monitoring device for cell microdamage induction and bright field monitoring method
  • Monitoring device for cell microdamage induction and bright field monitoring method

Examples

Experimental program
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Embodiment 1

[0039] Please refer to figure 1 and figure 2 , this embodiment discloses a monitoring device for cell micro-damage induction, which mainly includes a sampling unit 1, an ultrasonic generating unit 3, an optical detection unit 4 and a processing unit 5, which will be described separately below.

[0040] The sampling unit 1 has a detection table 11 for placing the sample container 21, and the detection table 11 may have a groove or a bracket adapted to the sample container 21, so that the sample container 21 can be placed stably. The sample container 21 can be a sample cup or a petri dish, which is used to accommodate the sample 22 to be tested formed by mixing the cell suspension solution and the cell microdamage solution. The sample 22 to be tested includes a plurality of cells and several microbubbles attached to each cell . Among them, the cell suspension is a solution of a certain concentration of living cells, which contains many living cells; the cell microinjury solut...

Embodiment 2

[0084] On the basis of the monitoring device disclosed in Example 1, this example discloses a bright field monitoring method for cell damage, the bright field monitoring method is mainly in figure 1 and figure 2 The application is carried out on the processing unit 5 in the

[0085] In this example, please refer to Figure 8 , the bright field monitoring method for cell micro-damage includes steps 110-160, which will be described respectively below.

[0086] Step 110, acquiring a bright-field image of micro-damaged cells in the sample to be tested.

[0087] For the monitoring device in Embodiment 1, see figure 1 and figure 2 , through the camera 42 in the optical detection unit 4, some images after the cells in the sample 22 to be tested are slightly damaged are captured, and a bright field image is formed and stored in the processing unit 5. Then, the processing unit 5 can obtain by reading One or more brightfield images. It should be noted that the bright field image...

Embodiment 3

[0105] On the basis of the bright field monitoring method for cell micro-damage disclosed in the second embodiment, a monitoring device is disclosed in this embodiment, and the monitoring device 7 includes a memory 71 and a processor 72 .

[0106] In this embodiment, the memory 71 and the processor 72 are the main components of the monitoring device 7. Of course, the monitoring device 7 may also include some detection components and execution components connected to the processor 72. For details, refer to the first embodiment above, here No more details.

[0107] Wherein, the memory 71 can be used as a computer-readable storage medium, and is used for storing a program here, and the program can be a program code corresponding to the bright field monitoring method in the second embodiment.

[0108] Wherein, the processor 72 is connected with the memory 71, and is used to execute the program stored in the memory 71 to realize the bright field monitoring method disclosed in the s...

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Abstract

The invention relates to a monitoring device for cell microdamage induction and a bright field monitoring method.The monitoring device comprises a sample introduction unit, an ultrasonic generation unit, an optical detection unit and a processing unit, and the processing unit inputs a bright field image into a first neural network; and performing re-optimization processing on the initial segmentation image about the cells and the microbubbles obtained by the feature segmentation processing to obtain a cell segmentation image and a microbubble segmentation image, performing feature extraction of target cells on the cell segmentation image, and performing feature extraction of target microbubbles on the microbubble segmentation image. And obtaining a cell microdamage characteristic parameter, a microbubble microdamage characteristic parameter and a joint microdamage characteristic parameter, inputting the microdamage characteristic parameters into a second neural network, and carrying out classification processing on microdamage characteristics to obtain a classification result of a target cell microdamage degree. According to the technical scheme, a novel cell microdamage monitoring mode is provided, and the classification result of the target cell microdamage degree can be obtained through accurate and refined analysis.

Description

technical field [0001] The present application relates to the technical field of medical detection, in particular to a monitoring device and a bright field monitoring method for cell micro-damage induction. Background technique [0002] Cell microdamage refers to the local damage induced on the cell membrane with micron-scale precision. This local damage will affect the integrity of the cell membrane, and then affect the functions of the cell such as material exchange, information transmission, immune response, cell division and differentiation. The degree of cell damage is related to the nature, intensity and duration of the induction method. Some induction methods will cause relatively weak reversible damage, while others will cause serious irreversible damage and even cell death. Cell micro-damage induction technology has important applications in the fields of cell biophysics research and cell repair medicine research. [0003] Currently, the ways to induce cell damage ...

Claims

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Application Information

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IPC IPC(8): G06K9/62G06T7/10G06V10/764
Inventor 胡亚欣李玲茜
Owner SHENZHEN UNIV
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