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Preparation system of stem cell exosome

A preparation system and exosome technology, applied in the field of stem cell exosome preparation system, can solve the problem that the centrifugal device cannot extract the cell fluid layer by layer

Pending Publication Date: 2022-04-12
深圳大学总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, this application provides a stem cell exosome preparation system to solve the technical problem that the centrifugal device cannot effectively and conveniently extract the cell fluid layer by layer

Method used

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  • Preparation system of stem cell exosome
  • Preparation system of stem cell exosome
  • Preparation system of stem cell exosome

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The present invention provides a preparation system of stem cell exosomes, referring to Figure 1-Figure 5 , as a specific embodiment, including a cell culture device 9, a filter device 8 and a cell centrifugation device 1, the cell centrifugation device 1 includes a cylindrical centrifugal chamber 10, a first Two side walls 11, a first driving device 12 for driving the centrifugal chamber 10 and the second side wall 11 to rotate synchronously, the second side wall 11 is a cylinder coaxially arranged with the centrifugal chamber 10 Shape, the inner surface of the second side wall 11 and the outer wall of the centrifugal chamber 10 are sealed and slidably fitted, the second side wall 11 is provided with a first bar-shaped through hole 110, and the centrifugal chamber 10 A second strip-shaped through-hole 100 is provided on the outer side wall of the centrifugal chamber 10 and the second strip-shaped through-hole 110 and the second strip-shaped through-hole 110 when the c...

Embodiment 2

[0056] The present invention provides a system for preparing stem cell exosomes. As a further improvement, the difference between this embodiment and Embodiment 1 is that the centrifugal device further includes a locking mechanism 6, and the bearing plate is connected to the second side The wall is integrally arranged, and a plurality of first guide holes 61 are arranged at intervals around the centrifugal chamber on the second side wall, and each of the first guide holes 61 is provided with a second guide hole 64 intersecting each other. A guide rod 62 is pierced in a guide hole, the guide rod is provided with a driving surface 620, and a guide block 65 is provided in the second guide hole, and the guide block 65 is provided with a first drive surface 620 to match. Two driving surfaces 650, the side wall of the centrifugal chamber is provided with the insertion holes 63 corresponding to the guide holes one by one, and also includes a driving assembly 66, the driving assembly c...

Embodiment 3

[0067] Embodiment 3 The present invention provides a stem cell exosome preparation system. It can be understood that, during high-speed rotation, high-frequency vibrations will occur in the centrifugal chamber and the second side wall. When the vibration amplitude is too large, the centrifugal device itself There is great harm, so it is necessary to eliminate the vibration; as a further improvement, the difference between this embodiment and embodiment two is that the reference figure 1 , Figure 11-Figure 13 , the centrifugal device also includes a stabilizing mechanism 7, and the stabilizing mechanism 7 includes a plurality of stabilizing components arranged at uniform intervals along the circumferential direction, and the stabilizing component includes a stabilizing block 71, and the stabilizing block has a sliding fit with the outer side wall of the second side wall The arc-shaped stable surface 710 is arranged in the guide cavity 73 of the housing 5, and the side of the s...

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Abstract

The present invention provides a stem cell exosome preparation system, which comprises a cell culture device, a filtration device and a cell centrifugation device, the cell centrifugation device comprises a cylindrical centrifugation cavity, a second side wall arranged around the outer side wall of the centrifugation cavity, and a first driving device for driving the centrifugation cavity and the second side wall to synchronously rotate, the second side wall is in a cylinder shape coaxially arranged with the centrifugal cavity, the inner side face of the second side wall is in sealing sliding fit with the outer side wall of the centrifugal cavity, a first strip-shaped through hole is formed in the second side wall, and a second strip-shaped through hole is formed in the outer side wall of the centrifugal cavity; when the centrifugal cavity and the second side wall relatively slide in the circumferential direction, the first strip-shaped through hole and the second strip-shaped through hole can partially coincide to form a liquid outlet, and when the centrifugal cavity and the second side wall relatively slide in the circumferential direction, the liquid outlet can move in the axial direction of the centrifugal cavity, and through the arrangement mode, the position of the liquid outlet can be conveniently controlled; therefore, the separated filtrate can be effectively and conveniently extracted in a layered manner.

Description

technical field [0001] The present invention generally relates to the technical field of cell exosome preparation, in particular to a preparation system of stem cell exosome. Background technique [0002] Exosomes (exosomes) are endogenous extracellular vesicles (extracellular vesicles, EVs) released by cells. and soluble protein), carbohydrates, lipids, RNA (mRNA, miRNA, lncRNA, etc.), DNA and other biologically active molecules are the media of intercellular communication and play an important role in many physiological and pathological processes. A new generation of nano drug carriers. [0003] In the preparation process of cellular exosomes, the cells need to be cultured first, and then the cultured cells are filtered, centrifuged, etc. During centrifugation, gradient centrifugation is required, and after each centrifugation, wait for the cell liquid to separate. After layering, it is necessary to extract the cell fluid of the upper layer or the lower layer, and the ex...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/00B04B7/00B04B9/02B04B9/08B04B11/04B04B15/00
Inventor 许霁虹黄海燕
Owner 深圳大学总医院
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