Prolactin gene SNP molecular marker related to Muscovy duck egg laying traits and application of prolactin gene SNP molecular marker
A technology of molecular markers and prolactin, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of long cycle, lack of systematic breeding, and uneven egg production traits of Muscovy ducks.
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[0032] 1 Materials and methods
[0033] 1.1 Animal samples
[0034] A total of 387 60-week-old female Muscovy ducks were selected (Guangdong Wenshi Southern Poultry Breeding Co., Ltd. Renma Breeding Duck Farm). 2 mL of subcutaneous venous blood was collected and stored at -80°C for DNA extraction. Record the pedigree information of the selected population and reproductive traits such as age at first lay, daily egg production, total egg production, and qualified egg production.
[0035] 1.2 Main instruments
[0036] BIO-RAD T100 PCR instrument (brand: BIO-RAD; item number: 1861096; American Bole), Beijing Liuyi DYCP-31DN nucleic acid electrophoresis instrument (brand: Beijing Liuyi; item number: 413-1402; Beijing Liuyi Biotechnology Co., Ltd. ).
[0037] 1.3 Main reagents
[0038] Rapid Blood Genomic DNA Extraction Kit (Brand: Novizyme; Item No.: DC111-01; Jiangsu Novozyme Biotechnology Co., Ltd.), 2xTaq MasterMix (with dye) (Brand: Kangwei Century; Item No.: CW0682; Jiang...
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