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Prolactin gene SNP molecular marker related to Muscovy duck egg laying traits and application of prolactin gene SNP molecular marker

A technology of molecular markers and prolactin, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of long cycle, lack of systematic breeding, and uneven egg production traits of Muscovy ducks.

Active Publication Date: 2022-04-15
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Although muscovy ducks have been bred on a large scale in China, their egg production performance is poor and lack of systematic breeding, resulting in uneven egg production characteristics of muscovy ducks, which affects the productivity and economic benefits of enterprises
[0003] Egg-laying traits are low-heritability traits. Breeding methods based on trait selection have the characteristics of long cycles, slow breeding progress, and difficult to achieve obvious results. As a result, it is very challenging to improve the egg-laying performance of Muscovy ducks.

Method used

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  • Prolactin gene SNP molecular marker related to Muscovy duck egg laying traits and application of prolactin gene SNP molecular marker
  • Prolactin gene SNP molecular marker related to Muscovy duck egg laying traits and application of prolactin gene SNP molecular marker
  • Prolactin gene SNP molecular marker related to Muscovy duck egg laying traits and application of prolactin gene SNP molecular marker

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Experimental program
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Embodiment

[0032] 1 Materials and methods

[0033] 1.1 Animal samples

[0034] A total of 387 60-week-old female Muscovy ducks were selected (Guangdong Wenshi Southern Poultry Breeding Co., Ltd. Renma Breeding Duck Farm). 2 mL of subcutaneous venous blood was collected and stored at -80°C for DNA extraction. Record the pedigree information of the selected population and reproductive traits such as age at first lay, daily egg production, total egg production, and qualified egg production.

[0035] 1.2 Main instruments

[0036] BIO-RAD T100 PCR instrument (brand: BIO-RAD; item number: 1861096; American Bole), Beijing Liuyi DYCP-31DN nucleic acid electrophoresis instrument (brand: Beijing Liuyi; item number: 413-1402; Beijing Liuyi Biotechnology Co., Ltd. ).

[0037] 1.3 Main reagents

[0038] Rapid Blood Genomic DNA Extraction Kit (Brand: Novizyme; Item No.: DC111-01; Jiangsu Novozyme Biotechnology Co., Ltd.), 2xTaq MasterMix (with dye) (Brand: Kangwei Century; Item No.: CW0682; Jiang...

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Abstract

The invention discloses a prolactin gene SNP (Single Nucleotide Polymorphism) molecular marker related to egg laying traits of Muscovy ducks and application of the prolactin gene SNP molecular marker, and belongs to the technical field of biology, the molecular marker comprises SNP5, SNP6 and / or SNP7; wherein the SNP5 is located at a Chr2: 48996109 site of a sequence as shown in SEQ ID No.1, and an allele is C > A; the SNP6 is located at a Chr2: 48996350 site of a sequence as shown in SEQ ID No.1, and an allele is T > C; the SNP7 is located at a Chr2: 48996351 site of a sequence as shown in SEQ ID No.1, and an allele is insertion / deletion; the invention can accurately identify the egg laying traits of Muscovy ducks of different weeks old, and provides a new molecular marker for molecular marker-assisted selective breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a prolactin gene SNP molecular marker related to egg-laying traits of muscovy ducks and an application thereof. Background technique [0002] Muscovy duck (Cairina moschata) is a special water bird, which comes from a different genus from the domestic duck. It has the advantages of strong heat resistance and high meat production, but its egg production performance is low. The peak egg production period of Muscovy ducks is between 35 and 53 weeks, covering most of the egg production period. Although muscovy ducks have been cultivated on a large scale in China, their egg production performance is poor and lack of systematic breeding, resulting in uneven egg production characteristics of muscovy ducks, which affects the productivity and economic benefits of enterprises. [0003] Egg-laying traits are low-heritability traits. The breeding method based on trait selection has the characte...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6869
Inventor 聂庆华张思雨徐海平郭利金徐翌斌黄育林叶茂
Owner SOUTH CHINA AGRI UNIV
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