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Method for detecting skatole in pig adipose tissue sample

A technology for tissue samples and adipose tissue, applied in the field of agriculture and food, can solve problems such as limitations, unpleasant operators, expensive detection methods, etc., and achieve the effect of robust equipment and high signal-to-noise ratio

Pending Publication Date: 2022-04-26
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of detection is expensive, limited, unpleasant for the operator, and only effective when a small number of carcasses need to be sorted, as the operator's olfactory receptors become saturated very quickly
Furthermore, this method produces highly variable results that are operator dependent and have only a 70% true positive rate

Method used

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  • Method for detecting skatole in pig adipose tissue sample
  • Method for detecting skatole in pig adipose tissue sample
  • Method for detecting skatole in pig adipose tissue sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0130] The experiments described below were carried out using the following materials:

[0131] - organic extracts of porcine adipose tissue samples either contaminated with or not contaminated with skatole;

[0132] - or hereinafter referred to as "standard solution" and includes a solution of synthetic skatole in acetonitrile.

[0133] one, Preparation of Organic Extracts and Standard Solutions of Porcine Adipose Tissue Samples

[0134] One 1- organic extract :

[0135] Organic extracts of porcine adipose tissue samples either contaminated with or not contaminated with skatole were prepared for each extract according to the following protocol.

[0136] A finely sliced ​​porcine adipose tissue sample was placed in a glass beaker and left at a temperature of 120°C for 1 hour. During this heating, the beaker was uncovered to allow water vapor to escape. Choose the size of the adipose tissue sample so that at least 5 mL of melted fat is collected at the end of this step....

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Abstract

The invention relates to a method for detecting the presence of skatole in a porcine adipose tissue sample, said method being characterized in that it comprises at least the following steps: a) subjecting an organic extract of said adipose tissue sample to an electrochemiluminescence reaction; b) measuring the luminous intensity during step a), and if the measured luminous intensity exceeds a threshold, deducing that skatole is present in the adipose tissue sample. The method can also determine the content of skatole in the presence of skatole in the organic extract. The application comprises the following steps: sorting complete boar carcasses in a slaughter house so as to remove parts with boar peculiar smell in meat to avoid consumption; research tools, in particular for studying factors affecting the skatole production in adipose tissue of a complete boar carcass in order to develop a method for preventing or reducing the offensive odor of a boar.

Description

technical field [0001] The present invention relates to the field of agro-food, and in particular to the production and distribution of pork. [0002] More specifically, the present invention relates to a method for detecting the presence of skatole or 3-methylindole (3-MIH) in a sample of porcine adipose tissue (tissus adipeux), if skatole or 3-methylindole is present Indole (3-MIH), its content was determined with very high sensitivity and very high specificity relative to other compounds that may also be present in porcine adipose tissue. [0003] Since skatole and androsterone together produce a strong and unpleasant odor known as boar taint, which is released when the meat of a whole boar is cooked, the invention is primarily applicable in slaughterhouses for sorting Whole boar carcasses (carcasses), the purpose is to isolate those carcasses of whole boars whose meat has boar taint and direct them to the appropriate processing circuit. [0004] The invention is also sui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/76
CPCG01N27/3277G01N21/76G01N1/4044G01N21/763G01N33/4833
Inventor E·斯科松M·哈默尔
Owner COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
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