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Preparation method of high-efficiency and low-cost bee peptide feed additive and feed additive

A low-cost technology for feed additives, applied in the field of preparation of feed additives and bee peptide feed additives, can solve the problems of difficulty in large-scale preparation and production of bee peptides and high production costs, reduce the cost of breeding feed, reduce the cost of raw materials, and increase the economy. effect of benefit

Pending Publication Date: 2022-04-29
ZHANGYE OLYMBEL BIO TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-efficiency and low-cost preparation method of honeybee peptide feed additive and feed additive to solve the technical problems of high production cost and difficulty in large-scale preparation and production of honeybee peptide in the prior art

Method used

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  • Preparation method of high-efficiency and low-cost bee peptide feed additive and feed additive
  • Preparation method of high-efficiency and low-cost bee peptide feed additive and feed additive
  • Preparation method of high-efficiency and low-cost bee peptide feed additive and feed additive

Examples

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Embodiment 1

[0026] This embodiment provides a high-efficiency and low-cost honeybee peptide feed additive, and its preparation process includes the following steps:

[0027] (1) Activation of the Bacillus subtilis strain capable of expressing honeybee peptide: Streak the Bacillus subtilis strain capable of expressing honeybee peptide (the strain constructed by the technical scheme published in the patent application No. 201410654343X) in LB solid medium , and cultivated at 35±0.5°C for 18 hours to obtain an activated strain;

[0028] (2) Preparation of laboratory seed liquid: the activated strain was inoculated in LB liquid medium, and shaken and cultivated at 35±0.5°C and 200r / min for 20h to obtain laboratory seed liquid;

[0029] (3) Preparation of primary fermentation broth: 0.1 liter of laboratory seed liquid was inoculated into 10 liters of primary fermentation broth medium for expansion. The nutrient addition ratio of 0.8% sodium chloride and 0.03% antifoaming agent by mass percent...

Embodiment 2

[0034] This embodiment provides a high-efficiency and low-cost honeybee peptide feed additive, and its preparation process includes the following steps:

[0035] (1) Activation of the Bacillus subtilis strain capable of expressing honeybee peptide: Streak the Bacillus subtilis strain capable of expressing honeybee peptide (the strain constructed by the technical scheme published in the patent application No. 201410654343X) in LB solid medium , and activated at 37±0.5°C for 18 hours to obtain an activated strain;

[0036] (2) Preparation of laboratory seed liquid: the activated strain was inoculated in LB liquid medium, and shaken and cultured at 37±0.5°C and 200 r / min for 20 hours to obtain laboratory seed liquid;

[0037] (3) Preparation of primary fermentation broth: 0.1 liter of laboratory seed liquid was inoculated into 10 liters of primary fermentation broth medium for expansion. The nutrient addition ratio of sodium chloride 0.8% and antifoaming agent 0.03% by mass perc...

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Abstract

The invention discloses a preparation method of a high-efficiency and low-cost bee peptide feed additive and the feed additive, and belongs to the technical field of industrial microorganisms. Through three-stage fermentation, the components of the first-stage fermentation culture medium and the second-stage fermentation culture medium are simpler than those of the third-stage fermentation culture medium, so that the raw material cost is reduced by 800-1000 yuan / ton in the fermentation cost in the production level, and the product quality is improved on the premise that the sterilization effect and the application effect of the prepared product are not reduced. The technical problems that in the prior art, the production cost is too high, and bee peptide is difficult to prepare and produce on a large scale are solved, the breeding feed cost is reduced, and economic benefits are increased.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganisms, and in particular relates to a preparation method of a high-efficiency and low-cost honeybee peptide feed additive and the feed additive. Background technique [0002] Clostridium welchicc, also known as Clostridium perfringens, is widely distributed in nature and is a Gram-positive conditional pathogen. The bacteria easily invade the animal body from the digestive tract or wounds, reproduce in large numbers on the small intestine and cecal chorion, and produce 12 kinds of enterotoxins such as α and β, which can quickly act on the duodenum, jejunum, and ileum of animals, causing tissue damage. Especially after being absorbed by the small intestine, it will cause edema, hemorrhage and necrosis in other organs. Clostridium welchii disease has acute onset, high mortality and strong infectivity, causing huge economic losses. In order to prevent and treat Clostridioides welchii dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/20A23K20/163A23K50/30A23K50/60C12N1/21C12R1/125
CPCA23K10/18A23K20/20A23K20/163A23K50/30A23K50/60C07K7/08
Inventor 武刚刘文庆胡海燕薛虎平李萍陈玉玲俞颖张丽张甲戌
Owner ZHANGYE OLYMBEL BIO TECH
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