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Device for enzymoimmunodiacolation detection and its production method

An immunodiafiltration and enzyme immunity technology, which is applied in the field of medical testing, can solve the problem that the detection sensitivity, accuracy, speed and simplicity cannot be well met, the stability and storage period of the enzyme immunodiafiltration detection device are affected, and the enzyme immunity is affected. The sensitivity and specificity of the immunodiafiltration detection device achieve the effect of simple structure, easy observation, simple and fast operation

Inactive Publication Date: 2004-03-31
郭占军
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Problems solved by technology

During the drying process of samples adsorbed by nitrocellulose membrane, if no stabilizer is added, irreversible denaturation or activity loss of antigens or antibodies in the sample solution will often occur, which affects the sensitivity and specificity of the enzyme immunofiltration detection device. sex
In addition, this method usually uses a liquid enzyme-labeled antigen or antibody as an enzyme label, which not only affects the stability and shelf life of the enzyme immunofiltration detection device, but also increases the number of operating steps.
It cannot meet the requirements of sensitivity, accuracy, speed and simplicity of today's immunological assays.

Method used

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  • Device for enzymoimmunodiacolation detection and its production method
  • Device for enzymoimmunodiacolation detection and its production method

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Embodiment Construction

[0012] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0013] As shown in the accompanying drawings, the present invention is a device for enzyme immunofiltration detection, which is mainly a multi-layer cassette structure composed of multi-layer filter paper and nitrocellulose membrane. The uppermost layer is the filter expansion layer 2, which is qualitative or quantitative Filter paper, used to expand serum or whole blood, also acts as a filter for blood cells and other particles. The second layer is the enzyme marker layer 3, and its support is qualitative or quantitative filter paper, and there are stabilized solid-phase enzyme markers on the filter paper. The preparation method is: first take 1-10mg of dry weight enzyme marker and 30-100mg Dissolve trehalose in 1ml of distilled water to prepare the spotting solution. You can also add 30-100mg of trehalose to 1ml of the solution containing 1-10mg / ml enzyme m...

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Abstract

The present invention relates to an enzyme immunodiacolation detection equipment and its preparation method. Said equipment possesses a box body whose interior is equipped with filter expansion layer, diacolation layer and waste liquor absorbing layer, and is characterized by also containing a solid phase enzyme labeled compound layer made of qualitative or quantitative filter paper. When it is used, the blood serum or whole blood sample can be added only in one run, it can be washed and color-developed, its sensitivity and accuracy are high, and can be stored for a long time (can be stored for 1-2 years at room temp.).

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to an enzyme immunofiltration detection device. The invention also relates to a method of making the device. Background technique [0002] Existing devices for enzyme immunofiltration detection mostly use antigens or antibodies to be directly spotted on nitrocellulose membranes, which are used as diafiltration membranes after drying. The preparation method is to directly spot the prepared antigen or antibody solution on the nitrocellulose membrane, and then dry it at room temperature or vacuum it from the back of the nitrocellulose membrane. Place multiple layers of water-absorbing filter paper under the nitrocellulose membrane, and then pack it into a small box of corresponding shape and size. During operation, add the serum to be tested to the sample point of the diafiltration membrane, wait for diafiltration, then add liquid enzyme markers, wait for diafiltration, then add washin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/535G01N33/543
Inventor 郭占军赵华郭爱芹杨焕云
Owner 郭占军
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