Multi-process composite in-situ biological 3D printing equipment and use method thereof

Abstract

The invention discloses multi-process composite in-situ biological 3D printing equipment and a using method thereof.The multi-process composite in-situ biological 3D printing equipment comprises a material forming system, a feeding system and a controller, the feeding system conveys materials to an electrostatic spinning forming module of the material forming system, and an electrostatic spinning film is formed under the action of a high-voltage electrostatic module of the controller; the electrostatic spinning process is realized; the feeding system conveys materials to an extrusion transfer printing forming module of the material forming system, and a hydrogel extrusion layer with a structure is formed under the combined action of extrusion force and a spray head, so that the extrusion transfer printing process is achieved. The transfer printing technology of the biological 3D printing technology, the electrostatic spinning technology and the extrusion molding technology are combined, the device has the advantages of being simple and reliable in structure, low in manufacturing cost, good in process controllability, high in automation degree and the like, the biological material can evenly act on the wound surface, in-situ preparation of the electrostatic spinning film is achieved, and the application prospect is wide. The composition of the electrostatic spinning technology and the extrusion molding technology can be realized according to the specific requirements of the wound surface.

Description

technical field [0001] The present invention relates to the technical field of biological 3D printing, and more specifically relates to a multi-process composite in-situ biological 3D printing device and its usage method. Background technique [0002] Burns are a common yet devastating injury that can cause pain, disability, and even death, and the treatment of burns and correction of skin defects remains a challenge. [0003] Current treatment methods mainly rely on autologous skin transplantation and engineered skin substitutes or replacement tissues. However, when faced with large burn wounds, the amount of healthy skin left is insufficient to be used in severe injuries. At this time, engineering Skin substitutes reflect its unique advantages. [0004] Different cell approaches that currently rely on patient-derived autologous cells or donor-derived allogeneic cells in the preparation of engineered surrogate skins have been proposed as alternative therapeutic approaches,...

AI Technical Summary

Problems solved by technology

[0006] However, most of the current extrusion molding and electrospinning technologies are applied on large-scale three-axis platforms. When burns and other wounds occur in outdoor environments, on-site bio-3D printing cannot be realized. Now some researchers have developed portable extrusion machines. Out-of-type biological 3D printing equipment, but there are shortcomings such as uneven accumulation of materials and inability to achieve patterning

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