Method for reducing abundance of antibiotic resistance gene in soil by using edible fungi

An antibiotic resistance, soil technology, applied in the biological field, can solve problems such as damage to soil quality

Pending Publication Date: 2022-05-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antibiotic resistance genes in the soil can be repaired by the adsorption of biochar, but too much biochar will damage the soil quality.

Method used

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  • Method for reducing abundance of antibiotic resistance gene in soil by using edible fungi
  • Method for reducing abundance of antibiotic resistance gene in soil by using edible fungi
  • Method for reducing abundance of antibiotic resistance gene in soil by using edible fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1, using the evaluation to reduce the abundance of antibiotic resistance genes in soil

[0033] 1. Materials and methods

[0034] 1. Test material

[0035] The soil used in the test was collected from vegetable greenhouses in the suburbs of Beijing and applied chicken manure organic fertilizer.

[0036] The edible fungus variety is Pleurotus ostreatus 4195, which is recorded in the article "Song Qinggang et al. Cotton dregs as a new type of cultivation substrate for Pleurotus ostreatus. Screening test of formula. Edible fungus Vegetables 2020.2", the public can obtain it from the applicant and can only be used to repeat the present invention For experimental use, not for other use. Pleurotus ostreatus 4195 is currently preserved in the Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences.

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Abstract

The invention discloses a method for reducing abundance of antibiotic resistance genes in soil by using edible mushrooms. The method for reducing the abundance of the antibiotic resistance gene in the soil comprises the following step: burying oyster mushroom sticks into the soil to be treated. The method provided by the invention is a method for reducing the abundance of the antibiotic resistance gene in the soil by utilizing proliferation of oyster mushroom hypha, and the effect of reducing the abundance of the antibiotic resistance gene is achieved by changing a soil microbial community structure through hypha growth. According to the method, high-temperature and fermentation treatment is not needed, implementation operation is easy and convenient, the abundance of antibiotic resistance genes in the soil can be reduced, edible mushroom sporocarp can be normally harvested, economic benefits are generated, and mushroom residues obtained after fruiting can increase nutritional ingredients such as organic matter of the soil.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for reducing the abundance of antibiotic resistance genes in soil by using edible fungi. Background technique [0002] Livestock and poultry manure, which is used as organic fertilizer for crops, is an important source of antibiotic resistance gene residues in the environment. Antibiotic resistance genes in the environment can be transmitted vertically through bacterial proliferation, and can also be transferred horizontally between species through mobile genetic elements, which further cause ecological and food safety problems and threaten human health. At present, there are mainly the following methods for the reduction of antibiotic resistance genes in the environment: high temperature (anaerobic) fermentation treatment; photodegradation; biofilm treatment (water system), etc. The antibiotic resistance genes in the soil can be repaired by the adsorption of biochar, but t...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 宋爽孙晓红刘宇王守现秦文韬韩梅琳荣成博高琪赵爽郭远宋庆港
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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