LOCKR-mediated CAR T cell recruitment

Abstract

The present invention discloses a method for isolating bioactive peptides and / or binding domains in an inactive ("off") state until binding to a second designed polypeptide, referred to as a key, only when a protein switch component is co-localized when bound to its target. The polypeptides induce protein switches that activate ("open") the conformational change of the bioactive peptide or binding domain, components of such protein switches, and uses thereof.

Description

[0001] cross reference [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 62 / 848840, filed May 16, 2019, and U.S. Provisional Patent Application Serial No. 62 / 964024, filed January 21, 2020, which are incorporated by reference The entire contents are incorporated herein. [0003] Federal Funding Statement [0004] This invention was made under Government Grant No. CHE-1629214 awarded by the National Science Foundation, Grant No. HDTRA1-18-1-0001 awarded by the Defense Threat Reduction Agency (DTRA), and Grant No. R01CA114536 awarded by the National Institutes of Health carried out with funding. The government has certain rights in this invention. [0005] Sequence Listing References Submitted Electronically via EFS-WEB [0006] This application contains a sequence listing submitted in the form of an electronic text file named "19-852-PCT_Sequence-Listing_ST25.txt", with a size of 32MB and created on May 14, 2020. Pursuant to 37 CFR §...

AI Technical Summary

Problems solved by technology

[0007] Biology is good at integrating multiple signals to control function; however, natural systems are highly evolved for specific functions, making them difficult to repurpose

Engineering systems capable of integrating binding events with predicted responsivity combinations remains an outstanding challenge

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