Gel microcapsule with cell recruitment function and preparation method thereof

A cell-recruiting and gel-based technology, which is applied in the field of cell-recruiting gel microcapsules and its preparation, can solve the problems of inability to realize the biological slow release of growth factors, and achieve prolonged physiological activity time, wide sources, good The effect of biocompatibility

Inactive Publication Date: 2013-06-26
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After direct compounding of cytokines and various scaffold materials, although better results can be achieved than simple scaffolds, this simple compounding (add-on or absorb-in) cannot achieve the biological sustained release of growth factors. The role of the drug delivery system is expected to achieve good tissue regeneration

Method used

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  • Gel microcapsule with cell recruitment function and preparation method thereof
  • Gel microcapsule with cell recruitment function and preparation method thereof
  • Gel microcapsule with cell recruitment function and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation of cell-recruiting gel microcapsules (colloidal solution) carrying stromal cell-derived factor-1α nanospheres and platelet-derived growth factor-BB nanospheres

[0051] Weigh 5 μg of stromal cell-derived factor-1α and 5 μg of platelet-derived growth factor-BB nanospheres and disperse them in 0.5 g of phosphate buffer, and use them as the water phase after they are completely dissolved; weigh 150 mg of glycidyl methacrylate Dextran and gelatin (glycidyl methacrylate dextran has a weight average molecular weight of 100-105 kDa, gelatin has a weight average molecular weight of 100 kDa, polypropylacrylamide has a weight average molecular weight of 17 kDa, glycidyl methacrylate dextran, gelatin and polypropylene The polymerization ratio of acrylamide is 1:0.6:0.05) dissolved in 5g of organic solvent (dichloromethane: acetone = 6:2) as the oil phase; mix the above water phase and oil phase, and ultrasonically disperse for 1 min to prepare an emulsion for...

Embodiment 2

[0052] Example 2: Preparation of cell-recruiting gel microcapsules (colloidal solution) carrying stromal cell-derived factor-1α nanospheres and platelet-derived growth factor-BB nanospheres

[0053] Weigh 10 μg of stromal cell-derived factor-1α and 10 μg of platelet-derived growth factor-BB nanospheres and disperse them in 1 g of phosphate buffer, and use them as the water phase after they are completely dissolved; weigh 300 mg of glycidyl methacrylate dextran and gelatin (glycidyl methacrylate dextran has a weight average molecular weight of 100-105 kDa, gelatin has a weight average molecular weight of 100 kDa, polypropylacrylamide has a weight average molecular weight of 17 kDa, glycidyl methacrylate dextran (Dex-GMA) , gelatin and polypropylacrylamide (the polymerization ratio is 1:1:0.07) dissolved in 10g of organic solvent (dichloromethane: acetone = 6:2) as the oil phase; mix the above water phase and oil phase, and ultrasonically disperse for 1min Prepare the emulsion a...

Embodiment 3

[0054] Example 3: Preparation of cell-recruiting gel microcapsules (colloidal solution) carrying stromal cell-derived factor-1α nanospheres and platelet-derived growth factor-BB nanospheres

[0055] Weigh 20 μg of stromal cell-derived factor-1α and 20 μg of platelet-derived growth factor-BB nanospheres and disperse them in 2 g of phosphate buffer, and use them as the water phase after they are completely dissolved; weigh 600 mg of glycidyl methacrylate dextran and gelatin (glycidyl methacrylate dextran has a weight average molecular weight of 100-105 kDa, gelatin has a weight average molecular weight of 100 kDa, polypropylacrylamide has a weight average molecular weight of 17 kDa, glycidyl methacrylate dextran (Dex-GMA) , gelatin and polypropylacrylamide (the polymerization ratio is 1:1.2:0.09) was dissolved in 20g of organic solvent (dichloromethane: acetone = 6:2) as the oil phase; the above water phase and oil phase were mixed and ultrasonically dispersed for 1min Prepare t...

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Abstract

The invention relates to the field of tissue regeneration biological material development, and discloses a gel microcapsule which carries a stroma cell-derived factor-1 alpha nanoparticles and a platelet-derived growth factor-BB nanoparticles and has a cell recruitment function and a preparation method thereof, and a gel microcapsule preparation with the cell recruitment function and a preparation method thereof. The gel microcapsule with the cell recruitment function and the preparation thereof can be applied to the dentistry to promote regeneration of endogenous periodontium by recruiting own stem cells of the organism. The gel microcapsule with the cell recruitment function is characterized by comprising the stroma cell-derived factor-1 alpha nanoparticles and the platelet-derived growth factor-BB nanoparticles, glycidyl methacrylate dextranum having the weight-average molecular weight of 100-105 kDa, gelatin having the weight-average molecular weight of 100 kDa, polypropylacrylamide having the weight-average molecular weight of 17 kDa, and a cosolvent, an emulsifier and a stabilizer.

Description

technical field [0001] The present invention relates to the field of research and development of tissue regeneration materials, in particular to a gel microcapsule with cell recruitment and a preparation method thereof carrying stromal cell-derived factor-1α nanospheres and platelet-derived growth factor-BB nanospheres. The gel microcapsule with cell recruitment is applied in the treatment of dental periodontal disease, which can recruit endogenous stem cells in the body and promote the regeneration of periodontal tissue. Background technique [0002] Periodontitis (periodontitis) is a chronic periodontal infectious disease worldwide, with a high incidence (more than 70% of middle-aged and elderly people) and great harm (the loss of periodontal support tissue caused by periodontitis is the cause of adult tooth loss main reason). The treatment of periodontitis and the restoration and regeneration of periodontal defects are problems of international concern, and the preventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61K9/50A61K47/36A61K47/42A61P1/02A61K38/18
Inventor 陈发明孙海花鲁红安莹高丽娜
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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