Method for rapid rooting and budding of lucuma nervosa seeds based on exogenous hormone treatment
A technology of exogenous hormones and lucuma, applied in seed immunization, seed and rhizome treatment, botanical equipment and methods, etc., can solve unfavorable lucuma grafting and breeding, low germination rate and seedling rate, slow rooting and sprouting of seeds, etc. problem, to achieve the effect of shortening the time for seed rooting and germination, shortening the seedling cultivation cycle, and promoting rooting and germination
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Embodiment 1
[0029] The screening of embodiment 1 exogenous hormone
[0030] The method for fast rooting and sprouting of lucuma seeds based on exogenous hormone treatment comprises the following steps:
[0031] (1) Seed selection: choose Xiantao No. 1 seeds with full grains, mature and healthy;
[0032] (2) Disinfection treatment: put the seeds into 2% sodium hypochlorite solution by volume fraction, soak and disinfect for 20 minutes, then clean them with warm water and dry them;
[0033] (3) Hormone soaking contrast experiment: put the sterilized seeds into different exogenous hormone solutions and clear water for 24 hours, and then take them out; that is, set up the following 4 groups of experiments: exogenous hormone combination GA 3 +BR, single hormone GA 3 , single hormone BR and clear water control group, each group treated 30 lucuma seeds, repeated 3 times:
[0034] ① Exogenous hormone combination GA 3 +BR:GA 3 (100mg / L): BR (0.05mg / L) = 1:1;
[0035] ②Single hormone GA 3 : ...
Embodiment 2
[0041] The screening of embodiment 2 hormone concentration
[0042] Step (1), (2) and (4) in embodiment 2 are identical with embodiment 1, and the difference of embodiment 2 and embodiment 1 is:
[0043] A, exogenous hormone solution selects hormone GA for use in the step (3) 3 Seed soaking in 3 concentrations, namely: 60mg / L, 80mg / L and 100mg / L. 30 lucuma seeds were soaked in these three concentrations respectively, soaked for 24 hours, and repeated 3 times. Observe the rooting and germination effect of seeds treated with different concentrations. See the comparative situation of seed rooting and germination effect figure 2 .
[0044] B, in the step (3), exogenous hormone solution selects 3 kinds of concentrations of hormone BR to soak seeds, is respectively: 0.05mg / L, 0.10mg / L and 0.15mg / L. 30 lucuma seeds were soaked in these three concentrations respectively, soaked for 24 hours, and repeated 3 times. Observe the rooting and germination effect of seeds treated with ...
Embodiment 3
[0048] The screening of embodiment 3 optimum soaking time
[0049] Step (1), (2) and (4) in embodiment 3 are identical with embodiment 1, and the difference of embodiment 3 and embodiment 1 is:
[0050] Use of exogenous hormones in combination with GA in step (3) 3 +BR:GA 3 (100mg / L): BR(0.05mg / L) = 1:1 to soak the seeds, set 3 different soaking times, 12h, 24h, 36h respectively, treat 30 lucuma seeds in each group, repeat 3 times. Observe the rooting and germination effects of seeds treated with different soaking times. The results showed that the seeds soaked for 24 hours had the best rooting and germination effect.
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