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Yeast recombinant human III-type triple helix collagen and preparation method thereof

A technology of collagen and triple helix, which is applied in the field of preparation of yeast recombinant human type III triple helix collagen and yeast recombinant human type III triple helix collagen

Pending Publication Date: 2022-05-13
JIANGSU TRAUTEC MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the research and development and design of yeast recombinant human triple-helical collagen as a substitute and supplement for existing collagen products has huge market potential and prospects. However, in the domestic market, there is no approved yeast recombinant triple-helical collagen biomaterial.

Method used

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  • Yeast recombinant human III-type triple helix collagen and preparation method thereof
  • Yeast recombinant human III-type triple helix collagen and preparation method thereof
  • Yeast recombinant human III-type triple helix collagen and preparation method thereof

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Embodiment Construction

[0052] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0053] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " Back", "Left", "Right", "Vertical", "Horizontal", "Top", "Bottom", "Inner", "Outer", "Clockwise", "Counterclockwise", "Axial", The orientation or positional relationship indicated by "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invent...

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Abstract

The invention provides a yeast recombinant human III type triple helix collagen and a preparation method thereof. The preparation method comprises the following steps: S1, constructing a pPICZ alpha B plasmid containing a human III type collagen alpha 1 chain; s2, constructing a double-promoter expression vector of the alpha subunit and the beta subunit of the human P4H; s3, co-transforming the double-promoter expression vector and the pPICZ alpha B plasmid into saccharomycetes, so as to obtain a co-expression strain; s4, screening a yeast recombination strain containing a P4H alpha subunit and a beta subunit from the co-expression strain; and S5, carrying out induced expression on the yeast recombinant strain to obtain the yeast recombinant human III-type triple helix collagen. According to the preparation method disclosed by the invention, the problem of pyrogen does not exist, the generation of immunogenicity is effectively avoided, and the interaction force, the thermal stability and the anti-enzymolysis level among collagen molecules are effectively improved.

Description

technical field [0001] The invention relates to the technical field of gene recombination, in particular to a preparation method of yeast recombinant human type III triple helix collagen and the yeast recombinant human type III triple helix collagen obtained by the preparation method. Background technique [0002] Collagen is the most abundant protein in humans and animals, accounting for 25-30% of the total protein in the body, accounting for about 6% of body weight. Collagen is an important structural protein in skin, bones, teeth, cornea, tendons, ligaments and various tissues and organs, which supports and protects body tissues. Collagen is the main component of the extracellular matrix, and its biological activity is mainly manifested in the promotion of cell adhesion, growth and differentiation, the response of cell signaling pathways, platelet aggregation, maintenance of cell and tissue organ functions, and damage repair. Collagen presents a three-strand helical wind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/81C12N15/12C12N15/53C12N15/66C07K14/78C12P21/02C12N1/19C12R1/645
CPCC12N9/0071C12N15/815C12N15/66C07K14/78C12P21/02C12Y114/11002
Inventor 张磊王丽萍李佳佳
Owner JIANGSU TRAUTEC MEDICAL TECH CO LTD
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