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Yeast recombinant human I-type triple helix collagen and preparation method thereof

A collagen, triple helix technology, used in animal/human proteins, biochemical equipment and methods, recombinant DNA technology, etc.

Pending Publication Date: 2022-07-22
JIANGSU TRAUTEC MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the research and development and design of yeast recombinant human triple-helical collagen as a substitute and supplement for existing collagen products has huge market potential and prospects. However, in the domestic market, there is no approved yeast recombinant triple-helical collagen biomaterial.

Method used

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  • Yeast recombinant human I-type triple helix collagen and preparation method thereof
  • Yeast recombinant human I-type triple helix collagen and preparation method thereof
  • Yeast recombinant human I-type triple helix collagen and preparation method thereof

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Embodiment Construction

[0051] The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, only used to explain the present invention, and should not be construed as a limitation of the present invention.

[0052] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", " Rear, Left, Right, Vertical, Horizontal, Top, Bottom, Inner, Outer, Clockwise, Counterclockwise, Axial, The orientations or positional relationships indicated by "radial direction", "circumferential direction", etc. are based on the orientations or positional relationships shown in the accompanying dr...

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Abstract

The invention provides a yeast recombinant human type I triple helix collagen and a preparation method thereof. The preparation method comprises the following steps: S1, constructing a pPICZ alpha B plasmid containing a human type I collagen alpha 1 chain; s2, constructing a double-promoter expression vector of the alpha subunit and the beta subunit of the human P4H; s3, transforming the double-promoter expression vector into saccharomycetes; s4, the pPICZ alpha B plasmid is transformed into saccharomycetes containing the double promoter expression vector, and a co-expression strain is obtained; s5, screening a yeast recombination strain containing a P4H alpha subunit and a beta subunit from the co-expression strain; and S6, carrying out induced expression on the yeast recombinant strain to obtain the yeast recombinant human I-type triple helix collagen. According to the preparation method disclosed by the invention, the problem of pyrogen does not exist, the generation of immunogenicity is effectively avoided, and the interaction force, the thermal stability and the anti-enzymolysis level among collagen molecules are effectively improved.

Description

technical field [0001] The invention relates to the technical field of gene recombination, in particular to a preparation method of yeast recombinant human type I triple helix collagen and the yeast recombinant human type I triple helix collagen obtained by the preparation method. Background technique [0002] Collagen is the most abundant protein in humans and animals, accounting for 25-30% of the total body protein and about 6% of body weight. Collagen is an important structural protein present in skin, bones, teeth, cornea, tendons, ligaments, and various tissues and organs, and functions to support and protect body tissues. Collagen is the main component of the extracellular matrix, and its biological activities are mainly manifested in the promotion of cell adhesion, growth and differentiation, response to cell signaling pathways, platelet aggregation, maintenance of cell and tissue organ functions, and damage repair. Collagen presents a triple helix winding structure ...

Claims

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Application Information

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IPC IPC(8): C12N15/81C12N1/19C07K14/78C12R1/84
CPCC12N15/815C07K14/78C12N2830/002
Inventor 张磊王丽萍李佳佳
Owner JIANGSU TRAUTEC MEDICAL TECH CO LTD
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