Soluble non-denatured II-type collagen and preparation method thereof

Abstract

The invention discloses soluble non-denatured II type collagen and a preparation method thereof. The method comprises the following steps: unfreezing cartilage, draining, weighing, homogenizing, sterilizing, disinfecting, removing impurities, carrying out enzymolysis, salting out, washing, drying and crushing to obtain soluble non-denatured II-type collagen powder. The soluble non-denatured II-type collagen has the advantages that the solubility is good, and the active part of the soluble non-denatured II-type collagen can be fully released in the intestinal tract; the soluble non-denatured II-type collagen is high in purity, a triple helix structure is kept complete, and the activity of improving arthritis is high; an extraction method of the soluble non-denatured II-type collagen is highin extraction rate, short in extraction time, high in safety, free of harmful substance residues and good in stability, so that the extraction of the soluble non-denatured II-type collagen is expanded to the production scale, and the production requirements are basically met. The technical scheme is simple and easy to implement, high in yield, short in time, low in cost and high in product activity.

Description

technical field [0001] The invention belongs to the technical field of health products, and in particular relates to a soluble non-denatured type II collagen and a preparation method thereof. Background technique [0002] Arthritis is a general term for various types of arthritic diseases. It generally refers to the occurrence of one or more joints in the human body, characterized by joint pain, swelling, stiffness, and limited mobility. Inflammatory erosion of joints, bone or cartilage Degenerative joint disease. Arthritis is related to various factors such as degenerative diseases and autoimmunity, and is not a single disease. The common ones are osteoarthritis, rheumatoid arthritis, gouty arthritis, etc. Among them, the incidence rate of osteoarthritis is the highest, especially among middle-aged and elderly people, the incidence rate can be as high as 60%. [0003] Osteoarthritis is a degenerative disease characterized by wear, destruction, and loss of articular cartil...

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Problems solved by technology

[0008] At present, there are some extraction methods of non-denatured type II collagen or preparation methods of related complexes containing non-denatured type II collagen at home and abroad, such as Schilling et al. (Schilling, Marvin L, Fafard, Richard D. Biologically active products : US 2010.) Non-denatured type II collagen was prepared by non-enzyme treatment method; domestic Liu Aiqing, Wang Haiyan, Zhang Guifeng et al. (Liu Aiqing, Wang Haiyan, Zhang Guifeng, etc. A cartilage extraction The preparation method of the product.), the cartilage is removed by manual membrane removal, papaya serum, pineapple serum and protease are used for enzymatic extraction, but manual membrane removal is time-consuming and laborious, and the above-mentioned non-denatured type II collagen products are all insoluble or poor solubility, which limits its efficacy and application in food; for the extraction of soluble non-denatured type II collagen, domestic Li Jie, Zhu Dakai and Gong Hui (Li Jie, Zhu Dakai, Gong Hui. Non-denatured II Type II collagen production method.), using pepsin to remove impurities from cartilage for 24 hours, and then perform enzymatic hydrolysis and extraction for 24 hours to obtain soluble non-denatured type II collagen, but the time for removing impurities and extraction is long, and the cost of removing impurities is also high; Sun Shengwei et al. (Sun Shengwei, He Jian, Liu Meijuan, et al. A non-denatured collagen extraction method and collagen identification method.), first remove impurities from cartilage with guanidine hydrochloride, Tris-HCl, Bis-Tris solution, imidazole buffer solution or trypsin culture solution for 24-48 hours, centrifuged to obtain insoluble matter, and then 24 hours of enzymatic hydrolysis, salting out and other steps to obtain non-denatured collagen, which has potential safety hazards of guanidine hydrochloride residues, and the removal of impurities and incubation are complicated and time-consuming , long enzymatic extraction time

Therefore, the current preparation methods of soluble non-denatured type II collagen have low extraction rate, long extraction time, low product purity, poor product stability and complicated process, which are not conducive to large-scale production.

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