Nucleic acid detecting method based on cascade photoelectric active material and triple helix molecular switch

A photoelectric activity, molecular switch technology, applied in the field of nucleic acid detection, can solve the problems of complex equipment, high cost, long analysis time, etc., and achieve the effect of enhancing photocurrent intensity, low price, good flexibility and operability

Inactive Publication Date: 2019-07-02
UNIV OF JINAN
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Problems solved by technology

[0003] So far, the methods used for nucleic acid detection include electrochemical method, electrochemiluminescence method, fluorescence luminescence method and colorime...

Method used

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  • Nucleic acid detecting method based on cascade photoelectric active material and triple helix molecular switch
  • Nucleic acid detecting method based on cascade photoelectric active material and triple helix molecular switch
  • Nucleic acid detecting method based on cascade photoelectric active material and triple helix molecular switch

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Embodiment

[0032] A method for detecting nucleic acid based on a cascaded photoelectric active material and a triple-helix molecular switch is used in HIV-1, which is characterized in that it includes the following steps:

[0033] (1) Use Adobe Illustrator CS4 software to design the working layer and auxiliary layer of the microfluidic paper chip. The circular area in the middle of the working layer and the auxiliary layer is the hydrophilic area, and the rest is the wax-printed hydrophobic area. The diameter of the hydrophilic area of ​​the working layer is The size is 6 mm, and the diameter of the hydrophilic area of ​​the auxiliary layer is 8 mm; the carbon working electrode is printed on the hydrophilic area of ​​the working layer by screen printing, and the Ag / AgCl reference electrode and carbon counter electrode are printed on the hydrophilic area of ​​the auxiliary layer ;

[0034] The steps of preparing the working electrode for growing gold nanoparticles in the hydrophilic region ...

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Abstract

The invention discloses a nucleic acid detecting method based on a cascade photoelectric active material and a triple helix molecular switch. A semiconductor ZnO nano material and CdTe quantum dots with different particle diameters are modified on the surface of an electrode to form a cascade structure, which greatly enhances the photocurrent intensity. Through the formation and disassembly of triple helix molecules, the superiority of the triple helix molecular switch opens up new strategies for the high specificity detection of nucleic acids, and ultrasensitive detection of nucleic acids isrealized by means of an electrochemical workstation. According to the invention, a photoelectrochemical sensor can also be used for the detection of various nucleic acids by changing the sequence of the adapted DNA; and the method has the advantages of low cost, high sensitivity and rapid response, and has an important application prospect in biological analysis and clinical diagnosis.

Description

technical field [0001] The invention relates to a nucleic acid detection method with low cost, simple operation and high sensitivity, belonging to the technical field of nucleic acid detection. Background technique [0002] Nucleic acid is an important carrier of genetic information in organisms. It plays a role in storing and disseminating genetic information in life activities, and determines a series of major life phenomena, such as the growth, inheritance and variation of organisms. Therefore, accurate, rapid and specific nucleic acid determination has become a core issue in modern life sciences for gene therapy, clinical diagnosis and cancer research. [0003] So far, the methods used for nucleic acid detection include electrochemical method, electrochemiluminescence method, fluorescence luminescence method and colorimetric method. Although these methods for detecting nucleic acid have their own advantages, they also have high cost and long analysis time. , complex equ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/3278G01N27/416
Inventor 葛慎光王绍鹏赵金歌李娜王芳芳付翠萍于京华
Owner UNIV OF JINAN
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