Substrate for detecting various inflammatory factors, preparation method, kit and application

Abstract

The invention belongs to the technical field of molecular biological detection, and discloses a substrate for detecting multiple inflammatory factors, a preparation method, a kit and application, multiple recombinant protein capture antibody bar codes are fixed on the substrate through a Lys-Au NPs (at) MoS2 nano composite material, the kit is used for detecting the multiple inflammatory factors, and the multiple inflammatory factors can be detected through the multiple recombinant protein capture antibody bar codes. Comprising a substrate, a detection sample loading chip, a plurality of biotin-labeled monoclonal detection antibodies, APC-labeled streptavidin, a working solution and a plurality of antigens. The preparation method of the Lys-Au NPs (at) MoS2 nano composite material on the substrate, disclosed by the invention, is novel, the materials are cheap, and the preparation process is simple and easy to operate; the substrate and the kit provided by the invention can be used for simultaneously detecting various inflammatory factors and are beneficial to improving the detection efficiency; the detection sensitivity is high, the consumed time is short and the detection cost is low.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and in particular relates to a substrate for detecting various inflammatory factors, a preparation method, a kit and an application. Background technique [0002] Inflammation (inflammation): It is usually called "inflammation", which is a defense response of the body to stimulation. It can be infectious inflammation due to infection or non-infectious inflammation not due to infection. Normally, inflammation is beneficial and is the body's automatic defense response, but sometimes it is also harmful, such as attacks on the body's own tissues, inflammation in transparent tissues, etc., and inflammation is not limited to infection and pathophysiological processes of trauma. [0003] In recent years, with the in-depth research on inflammatory factors, it has been found that there is a complex network connection between inflammation and various diseases, such as atherosclerosis,...

AI Technical Summary

Problems solved by technology

However, the experimental process of this method is cumbersome, the detection object is single, and the final detection is achieved through the Raman enhancement effect of gold and silver nanoparticles during the experimental process, which greatly reduces the work efficiency.

In general, although the methods developed in the past have good specificity, when the concentration of the detected substance is trace, the detection accuracy is often required to be very high, and several markers are often required to assist detection in clinical practice to achieve the purpose of identifying the cause

Images