Application of stem cell conditioned medium in preparation of anticancer composition, anticancer composition and application
A conditioned medium and stem cell technology, applied in the biological field, can solve the problems of normal cell death, high price, affecting the normal physiological function of patients, etc., and achieve the effect of inhibiting the growth of cancer cells and high safety.
Pending Publication Date: 2022-05-17
亚芯沛妍(上海)生物科技有限公司
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Problems solved by technology
[0003] Although the above methods can all play an anticancer effect, they generally have strong side effects. While removing or inhibiting cancer cells, they will also affect the growth of normal cells in the human body at the same time, and even cause the death of normal cells, which in turn affects the normal health of patients. Physiology that makes cancer patients more vulnerable
[0004] Although there are currently targeted drugs with higher specificity developed for cancer cells, not every cancer has a corresponding targeted drug, and the existing targeted drugs are generally expensive due to reasons such as difficult preparation and low yield. , will cause a great economic burden to patients; therefore, there is an urgent need to develop an anti-cancer composition that can be applied to a variety of cancers and has high specificity for cancer cells
Method used
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Embodiment 1
[0030] A stem cell conditioned medium, specifically Wharton gel mesenchymal stem cell conditioned medium, prepared through the following steps:
[0031] First set the number of cells to 2X10 5 Wharton gel mesenchymal stem cells cultured in 75cm 2 Add 10mL of α-MEM complete medium to the flask (cell culture flask), so that the cell concentration of Wharton's gel mesenchymal stem cells is 2X10 4 a / ml;
[0032] Among them, Wharton's Jelly mesenchymal stem cell (WJMSC for short) was obtained from the Biological Resource Conservation and Research Center (BCRC) of the Food Industry Development Institute of Taiwan, China, and its BCRC number is RM60596 .
[0033] Then at 37°C, 5% carbon dioxide (CO 2 ) concentration of culture conditions for pre-cultivation for 3-5 days, the application only takes 4 days as an example (when the number of days of culture is too small, the tumor suppressor hormone secreted by the stem cells is insufficient, and the number of days is too many and th...
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The invention relates to the technical field of biology, in particular to application of a stem cell conditioned culture medium in preparation of an anti-cancer composition, the anti-cancer composition and application of the anti-cancer composition. The stem cell conditioned culture medium can be combined with cytohormone to prepare an anticancer composition for inhibiting the growth of cancer cells, and the stem cell conditioned culture medium is a Warton type gel mesenchymal stem cell conditioned culture medium. The cytohormone is one or more of a free bone shaping protein-4, a Dickkopf related protein, an interferon beta and a tumor necrosis factor related apoptosis inducing ligand. According to the invention, through transformation of the stem cell conditioned culture medium and combination of the stem cell conditioned culture medium and the cytohormone, the growth of cancer cells is inhibited, and the cytohormone used in the invention does not influence the growth of normal cells, so that the stem cell conditioned culture medium has extremely high safety.
Description
technical field [0001] This application relates to the field of biotechnology, more specifically, it relates to the application of stem cell conditional medium in the preparation of anticancer composition, anticancer composition and application. Background technique [0002] Cancer is a disease in which cells proliferate abnormally and affect the normal physiological functions of organisms. In the past, cancer treatment methods mainly included surgical resection of cancer cell tissue, or radiation therapy, drug therapy, etc., to inhibit the growth of cancer cells. growth and induce cancer cell death. [0003] Although the above methods can all play an anticancer effect, they generally have strong side effects. While removing or inhibiting cancer cells, they will also affect the growth of normal cells in the human body at the same time, and even cause the death of normal cells, which in turn affects the normal health of patients. Physiology, making cancer patients more vulne...
Claims
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IPC IPC(8): A61K45/06A61K38/21A61K38/18A61K38/17A61K35/28A61P35/00
CPCA61K45/06A61K35/28A61K38/215A61K38/1875A61K38/1709A61P35/00A61K2300/00
Inventor 庄沛荃
Owner 亚芯沛妍(上海)生物科技有限公司