Application of Sorbic acid in preparation of anti-coronavirus infection medicine
A coronavirus, drug technology, applied in the direction of antiviral agents, resistance to vector-borne diseases, anhydride/acid/halide active ingredients, etc., can solve problems such as unseen Sorbicacid
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Embodiment 1
[0032] Time-dependent fluorescence intensity of substrate peptides metabolized by 3C-like proteases under different inhibitor concentrations
[0033] The specific implementation process is:
[0034] 1) The SARS-Cov-2 3C-like protease and substrate peptide stock solutions are stored in a -80°C refrigerator;
[0035] 2) Thaw the SARS-Cov-2 3C-like protease in a cryopreservation plate (-4 to 4°C) at room temperature, take 1uL and dilute it in 98uL borate borax buffer (PH=7.4), and add it to the detection plate;
[0036] 3) Add 1uL of different concentrations of inhibitors (Sorbic acid, with concentrations of 0, 0.1mM, 0.4mM, 1mM, 2.5mM) into the solution obtained in the above step (2);
[0037] 4) Add 1uL of the same concentration of substrate peptide (0.5mM) to the solution obtained in the above step (3), incubate at 37°C with a fluorescent microplate reader, and monitor the fluorescence emission at 342nm excitation and 496nm with a fluorescent microplate reader , detect while...
Embodiment 2
[0040] Determination of the inhibitory ability of compound Sorbic acid to SARS-Cov-2 3C-like protease
[0041] The specific implementation process is as follows:
[0042] 1) The SARS-Cov-2 3C-like protease and substrate peptide stock solutions are stored in a -80°C refrigerator;
[0043] 2) Thaw the SARS-Cov-2 3C-like protease in a cryopreservation plate (-4 to 4°C) at room temperature, take 1uL and dilute it in 98uL borate borax buffer (PH=7.4), and add it to the detection plate;
[0044] 3) Add 1uL of different concentrations of inhibitors (Sorbic acid, with concentrations of 0, 0.1mM, 0.4mM, 1mM, 2.5mM) into the solution obtained in the above step (2);
[0045] 4) Add 1uL of the same concentration of substrate peptide (0.5mM) to the solution obtained in the above step (3), incubate at 37°C with a fluorescent microplate reader, and monitor the fluorescence emission at 342nm excitation and 496nm with a fluorescent microplate reader , detect while incubating, incubate for 1 ...
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