Copper sulfide/manganese dioxide composite material and preparation method and application thereof

A technology of manganese dioxide and composite materials, applied in the field of nano-biomedical materials, can solve problems such as off-target, abnormal differentiation, and tumor induction, and achieve the effects of anti-inflammatory chondrogenic differentiation, enhancing efficiency, and promoting biological functions

Pending Publication Date: 2022-05-24
澳汀斯(广州)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, MSCs also have problems such as off-target, abnormal differentiation, and even tumor induction after injection.

Method used

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  • Copper sulfide/manganese dioxide composite material and preparation method and application thereof
  • Copper sulfide/manganese dioxide composite material and preparation method and application thereof
  • Copper sulfide/manganese dioxide composite material and preparation method and application thereof

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Embodiment 1

[0046] The preparation method of the copper sulfide / manganese dioxide composite material described in this embodiment includes the following steps:

[0047] (1) Dissolve 188 mg of copper nitrate and 363 mg of L-cysteine ​​in 200 mL of deionized water and mix to form a mixed solution, dissolve 744 mg of sodium thiosulfate pentahydrate in 10 mL of deionized water, and then slowly add it dropwise into the mixed solution, The reaction was stirred at 90°C for 2 hours, cooled to room temperature, centrifuged at 4000rpm for 15min, washed 5 times with deionized water or absolute ethanol, and dried to obtain copper sulfide;

[0048] (2) the copper sulfide and potassium permanganate obtained in the step (1) were mixed and reacted for 1 hour according to the mass ratio of 1:1, and CuS / MnO was obtained after centrifugation, washing and drying 2 Material;

[0049] (3) CuS / MnO obtained in step (2) 2 The material is connected with stem cell targeting peptide through EDC / NHS reaction to obt...

Embodiment 2

[0051] The preparation method of the copper sulfide / manganese dioxide composite material described in this embodiment includes the following steps:

[0052] (1) Dissolve 188 mg of copper nitrate and 363 mg of L-cysteine ​​in 200 mL of deionized water and mix to form a mixed solution, dissolve 744 mg of sodium thiosulfate pentahydrate in 10 mL of deionized water, and then slowly add it dropwise into the mixed solution, The reaction was stirred at 90°C for 2 hours, cooled to room temperature, centrifuged at 4000rpm for 15min, washed 5 times with deionized water or absolute ethanol, and dried to obtain copper sulfide;

[0053] (2) the copper sulfide and potassium permanganate obtained in the step (1) were mixed and reacted for 1 hour according to the mass ratio of 1:0.5, and CuS / MnO was obtained after centrifugation, washing and drying 2 Material;

[0054] (3) CuS / MnO obtained in step (2) 2 The material is connected with stem cell targeting peptide through EDC / NHS reaction to o...

Embodiment 3

[0056] The preparation method of the copper sulfide / manganese dioxide composite material described in this embodiment includes the following steps:

[0057] (1) Dissolve 188 mg of copper nitrate and 363 mg of L-cysteine ​​in 200 mL of deionized water and mix to form a mixed solution, dissolve 744 mg of sodium thiosulfate pentahydrate in 10 mL of deionized water, and then slowly add it dropwise into the mixed solution, The reaction was stirred at 90°C for 2 hours, cooled to room temperature, centrifuged at 4000rpm for 15min, washed 5 times with deionized water or absolute ethanol, and dried to obtain copper sulfide;

[0058] (2) the copper sulfide and potassium permanganate obtained in the step (1) were mixed and reacted for 1 hour according to the mass ratio of 1:1.5, and CuS / MnO was obtained after centrifugation, washing and drying 2 Material;

[0059] (3) CuS / MnO obtained in step (2) 2 The material is connected with stem cell targeting peptide through EDC / NHS reaction to o...

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Abstract

The invention discloses a copper sulfide/manganese dioxide composite material and a preparation method and application thereof. The copper sulphide/manganese dioxide composite material comprises copper sulphide, manganese dioxide coating the surface of the copper sulphide, and stem cell targeting peptide modified on the surface of the manganese dioxide. The copper sulfide/manganese dioxide composite material disclosed by the invention has the capabilities of promoting MSCs migration, resisting inflammation and chondrogenic differentiation, also has the activity of superoxide dismutase and catalase, and can improve the cell activity of MSCs in an RA oxidative stress microenvironment. And the stem cell targeting peptide on the surface can enhance the mesenchymal stem cell targeting capability of the material and enhance the stem cell engineering efficiency of the nano material. The mesenchymal stem cells engineered by the copper sulphide/manganese dioxide composite material can obviously enhance the cell migration, anti-inflammatory and chondrogenesis capabilities of the stem cells, especially the capabilities of resisting inflammatory environment and resisting the influence of ROS on the cells.

Description

technical field [0001] The invention belongs to the technical field of nanometer biomedical materials, and in particular relates to a copper sulfide / manganese dioxide composite material and a preparation method and application thereof. Background technique [0002] Rheumatoid arthritis, or RA for short, is a chronic systemic autoimmune disease with complex etiology, with a global prevalence of about 0.5%-1%. It is estimated that there are at least 5 million RA patients in my country, and it is one of the most common clinical joint and bone diseases. The main pathological features of RA are the formation of synovial pannus and persistent synovitis in multiple joints throughout the body, which in turn invades cartilage and bone, leading to loss of joint function and a high disability rate. There is still no effective treatment for RA, and drug therapy is mainly used to relieve pain and delay the disease process. At present, there are four main types of RA treatment drugs, na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/02A61K35/28A61P19/02A61P29/00
CPCA61K47/42A61K47/02A61K35/28A61P19/02A61P29/00
Inventor 陆遥丁长海
Owner 澳汀斯(广州)生物医药科技有限公司
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