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Efficient phosphate-solubilizing absidia and application thereof

A high-efficiency technology, applied in the field of microorganisms, to achieve the effects of promoting improvement, improving soil quality, and facilitating fermentation production

Active Publication Date: 2022-05-27
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reports on phosphorus-solubilizing fungi mostly focus on Aspergillus and Penicillium. For example, CN202111352520.5 discloses Aspergillus niger (Aspergillus niger) H1 with phosphorus-solubilizing effect, and CN201711354460.4 discloses that it has high-efficiency phosphorus-solubilization, potassium-solution, and high-efficiency degradation Aspergillus niger (Aspergillus niger) CS-1 of cellulose ability, CN201911377062.3 discloses a kind of Penicillium citrinum (Penicillium citrinum) PSF, CN201710197028.2 discloses understanding of Penicillium citrinum (Penicillium citrinum) QM-6 and QM-10 etc., no reports of Absidia strains having phosphorus-solubilizing properties were found after searching

Method used

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  • Efficient phosphate-solubilizing absidia and application thereof
  • Efficient phosphate-solubilizing absidia and application thereof
  • Efficient phosphate-solubilizing absidia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation and identification of strains

[0025] 1. Strain 2-5 separation

[0026] Plough mold strain 2-5 of the present invention is obtained by isolating the dead Tianniu from the masson pine forest farm in Yantai, Shandong Province, using dilution plate method. Here's how:

[0027] 1.1 Isolation of bacterial sample preparation

[0028] The collected dead tianniu was cleaned with distilled water, and disinfected with the surface of the cotton ball containing 75% alcohol 3 to 5 times; under sterile conditions, it was cut with scissors and put into a sterile mortar filled with 10mL of sterile water and a little sterilized quartz sand, fully ground into a homogeneous slurry with a grinding rod, and aspirated 5mL in a sterile test tube with a pipette, that is, a bacteriological sample.

[0029] 1.2 Dilute the coating

[0030] Use the 10-fold dilution method to dilute the samples sequentially to 10-1 ～10 -6 Dilution of the sample; respectively from 10 -4 、10 -5 and ...

Embodiment 2

[0055] Example 2: Determination of the efficient phosphorus solution capacity of Ploughweed mold 2-5

[0056] 1. Qualitative determination of the ability of ploughshare mold 2-5 efficient phosphorus solution

[0057] (1) Detection method:

[0058] Inoculate P. tillerii 2-5 on a plate containing PDA medium, incubate at a constant temperature of 27 °C for 36 h, activate the strain; use a hole puncher to beat the bacterial cake (5 mm) at the edge of the activated Ploughshares 2-5, inoculate in the center of the tricalcium phosphate inorganic phosphorus solid culture plate, incubate at 27 °C for 24 h, observe the phosphorus dissolving ring, measure the colony diameter and the resulting transparent ring diameter, and each treatment is three parallel. The measured colony diameter and transparent ring diameter are averaged and the solubility index is calculated.

[0059] Solubility index = transparent ring diameter / colony diameter

[0060] (2) Analysis of results:

[0061] Inoculating T...

Embodiment 3

[0072] Example 3: Preparation of fermentation broth of Ploughshares 2-5

[0073] (1) Inoculate The 2-5 activated Ploughshares mold was inoculated on the surface of the PDA plate and cultured at a constant temperature of 27 °C for 48 h;

[0074] (2) The perforator beats the bacterial cake at the edge of the activated strain, inoculates it in seed liquid medium (PDB medium), and cultures 26-40h in 27 °C and 120r / min shaker bottles to obtain the seed solution of Ploughweed 2-5;

[0075] (3) Inoculate the seed solution prepared in step (2) into the fermentation medium, the inoculation mass ratio is 1:50, and the fermentation conditions are the same step (2), and the fermentation broth of Ploughshares 2-5 is obtained. The number of colonies is 1×10 9 CFU·mL -1 。

[0076]Fermentation medium: potato 200g, glucose (or sucrose) 15g, peptone 10g, water 1000mL, pH natural.

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Abstract

The invention discloses absidia capable of efficiently solubilizing phosphorus and application of the absidia. According to the invention, absidia sp. 2-5 with an efficient phosphate solubilizing effect is screened from automorphic longhorn beetles, and the preservation number of the absidia sp. 2-5 is CGMCC (China General Microbiological Culture Collection Center) NO.23216. After the strain treats a culture solution containing tricalcium phosphate, the phosphorus solubilizing amount of the strain reaches up to 2162.7 mg / L; pot experiments show that the plant height and the root length of mung bean seedlings which are treated by fermentation liquor of the strain and take tricalcium phosphate as a unique phosphorus source are respectively increased by 1.66 times and 2 times compared with those of a control group, and the mung bean seedlings show an efficient phosphorus-dissolving characteristic. And the absidia 2-5 also improves the soil quality by enhancing the activity of urease and acid phosphatase in the soil.

Description

Technical field [0001] The present invention relates to a strain of highly efficient phosphorus-soluble ploughweed mold and its applications, belonging to the field of microbial technology. Background [0002] Currently, there are 1.319 billion hm worldwide 2 43% of arable land is deficient in phosphorus; at least 70% of phosphate fertilizer is reported to be fixed by the soil after entering the soil, becoming a fixed form that is difficult to absorb and utilize by crops. The phosphorus content in the cultivated soil is generally not high, coupled with the fixation effect on phosphorus in the soil, resulting in low phosphate fertilizer utilization efficiency, which seriously restricts the development of efficient agriculture and green agriculture. [0003] Phosphorus phosphate bacteria is a general term for a class of microorganisms with phosphorus dissolving ability, which can transform insoluble phosphides in soil into soluble phosphides that can be directly absorbed and utiliz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C05G3/00C05G3/80C05G3/90C05G5/20C09K17/14C12R1/65
CPCC12N1/14C05F11/08C05G3/00C05G3/80C05G3/90C05G5/20C09K17/14Y02W30/40
Inventor 杨庆山张淑静王静臧真荣李永涛贾明任飞马安宝舒秀阁梁静孙超
Owner SHANDONG FOREST SCI RES INST
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