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Production method of high-efficiency phosphorus-dissolving Aspergillus japonicus agent with tolerance to heavy metals

A technology of Aspergillus and heavy metals, applied in the field of environmental biology

Inactive Publication Date: 2016-10-12
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no literature report on the production method of producing high-efficiency phosphorus-decomposing Aspergillus japonicus agent from sweet potato starch wastewater and fungus chaff waste

Method used

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  • Production method of high-efficiency phosphorus-dissolving Aspergillus japonicus agent with tolerance to heavy metals

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Effect test

Embodiment 1

[0024] Embodiment 1: Production of high-efficiency phosphorus-dissolving Aspergillus japonicus mold agent resistant to heavy metals

[0025] 1. Medium preparation:

[0026] 1) Strain preservation medium (solid, 1 L): 5 g of glucose, 18-20 g of agar, 40 g of leachate obtained by boiling and filtering potatoes, adding sweet potato starch wastewater to make up to 1 L, and natural pH;

[0027] 2) Strain activation medium (liquid, 1 L): 5 g of glucose, 40 g of leachate obtained by boiling and filtering potatoes, adding sweet potato starch wastewater to make up to 1 L, natural pH;

[0028] 3) Seed medium (liquid, 10 L): peptone 10 g, glucose 20 g, KH 2 PO 4 10 g, MgSO 4 ·7H 2 0.5 g, add sweet potato starch waste water and settle to 10 L, natural pH;

[0029] 4) Fermentation medium (liquid, 200 L): KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH;

[0030] All the above media were sterilized at 121°C for 15...

Embodiment 2

[0036] Embodiment 2: The growth-promoting effect of high-efficiency phosphorus-dissolving Aspergillus japonicus agent on Viburnum

[0037] After mixing the prepared Aspergillus japonica agent with the soil at a ratio of 1:20, 1:15, 1:10, and 1:5, plant the germinated Viburnum seeds, and add untreated The chaff residue fermented by Aspergillus japonicus was used as the control. 5 pots were treated in each pot, and 3 seedlings were planted in each pot, which were placed in the greenhouse for unified management and watered regularly. After 60 days of planting and emergence of Viburnum, the growth conditions are shown in Table 1. It can be seen that the plants inoculated with Aspergillus japonicus inoculum, the dry weight of roots and seedlings of each addition ratio and the height of seedlings and root length all increase compared with the control group, and as the addition increases, the dry weight of roots and seedlings root length and seedling height showed an increasing tre...

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Abstract

The invention relates to a method for producing a high-efficiency phosphorus-dissolving Japanese mold agent with the characteristic of tolerance to heavy metals by using sweet potato starch wastewater and fungus chaff waste residue, and belongs to the field of environmental biotechnology. Efficient Phosphorus Solubilizing Aspergillus japonicus ( Aspergillus japonicus ) was isolated and screened by our laboratory from the surface soil of the lead-zinc mine in Huayuan County, Xiangxi Prefecture, Hunan Province, and has the characteristics of tolerance to various heavy metals. The method mainly uses sweet potato starch wastewater and fungus chaff waste residue as main medium raw materials, and adopts a liquid-solid joint fermentation method to produce a high-efficiency phosphorus-solubilizing Aspergillus japonicus fungus that is resistant to heavy metals. The production method of the bacterial agent comprises six steps: culture medium preparation, bacterial strain activation, liquid seed preparation, liquid state fermentation, solid state fermentation, and post-treatment. The technical scheme of the present invention not only provides a new way for the resource utilization of high-concentration sweet potato starch wastewater and fungus chaff waste residue, but also the Japanese Aspergillus agent produced is environmentally friendly and has low production cost, and the product also has the characteristics of significantly improving the soil environment Quality, the function of promoting plant growth.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a method for producing a high-efficiency phosphorus-solubilizing Aspergillus japonicus agent capable of resisting heavy metals by using sweet potato starch wastewater and fungus chaff waste residue as main culture medium raw materials through liquid-solid joint fermentation. Background technique [0002] Phosphorus is an essential element for plant growth, second only to nitrogen in importance. However, the nitrogen elements needed by plants can be realized by converting nitrogen in the air into nitrate nitrogen through nitrogen-fixing bacteria and nitrifying bacteria in the soil and roots, but the required phosphorus elements can only be met by absorbing soluble phosphorus in the soil. In the soil, especially in the mining area, due to the presence of a large amount of heavy metals, and the heavy metals are extremely easy to form insoluble phosphoric acid c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/66
Inventor 不公告发明人
Owner BEIJING FORESTRY UNIVERSITY
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