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Salt-tolerant trichoderma pleuroticola strain and application thereof

A technology of Trichoderma pedicularis and strains, applied in application, chemicals for biological control, biocides, etc., can solve the problems of difficult crop absorption and utilization of phosphate, and achieve accelerated prevention and control of plant diseases, good application prospects, and stress resistance. strong effect

Active Publication Date: 2015-05-27
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In my country, 74% of the arable land is deficient in phosphorus, and the seasonal utilization rate of the applied phosphorus fertilizer by crops is only 5% to 10%, and more than 95% of the phosphorus and Ca in the soil are 2+ , Fe 2+ , Fe 3+ 、A1 3+ Combined to form insoluble phosphate, which is difficult for crops to absorb and utilize

Method used

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  • Salt-tolerant trichoderma pleuroticola strain and application thereof
  • Salt-tolerant trichoderma pleuroticola strain and application thereof
  • Salt-tolerant trichoderma pleuroticola strain and application thereof

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Effect test

Embodiment 1

[0029] 1. Isolation and purification of Trichoderma pleurotica (T.pleuroticola) HF01

[0030] Trichoderma pleuroticola (T.pleuroticola) HF01 of the present invention is obtained from the coastal wetland soil by using the dilution plate method and the plate streaking method to separate and obtain, and the separation method is as follows:

[0031] (1) Isolation of Trichoderma strains: collection of saline soil samples, selecting plots planted with different types of crops in different saline areas of the country, using 5-point sampling method, and collecting the surrounding and central depths of the plots in the range of 10-20cm Appropriate amount of soil inside, mix in equal amount. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place in a shaker at 30°C at 150rpm for 10min, take 100μL of 10 -2 、10 -3 、10 -4 The dilution was spread on the PDA medium plate, and each gradient was spread in three parallels. After ...

Embodiment 2

[0053] 1. Fermentation process of Trichoderma pleurotus

[0054] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0055] A large amount of solid fermentation medium formula (mass percentage):

[0056] Solid material: composed of rice bran, corn flour and bran with a mass ratio of 75:10:15;

[0057] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0058] The solid-to-liquid ratio is 1:1.8 (mass ratio)

[0059] Mass solid fermentation process of Trichoderma pleuroticola (T.pleuroticola) HF01:

[0060] ① Strain seed liquid culture: Pick a small amount of spores of Trichoderma pleuroticola (T.pleuroticola) HF01 from the slant of the test tube, transfer them to PDB liquid medium, and culture them on a shaker at 28°C for 3-5 days, and this is the seed liquid.

[0061] ②Cultivation of solid production strains: inoculate 10% of the seed solution into solid medium (50...

Embodiment 3

[0064] Trichoderma pleuroticola (Trichoderma pleuroticola) HF01 cotton plot experiment

[0065] Test material: Trichoderma pleuroticola (Trichoderma pleuroticola) HF01 fermentation liquid (the seed liquid obtained in the above step ①), which has the effect of dissolving phosphorus, and the effective number of viable bacteria (viable spores) is greater than 2×10 8 cfu / ml.

[0066] Test time: from April 2012 to October 2012

[0067] Test site: Cotton Center Test Station, Shandong Academy of Agricultural Sciences, Linqing City

[0068] Test crop: Cotton (Lumianyan No. 28)

[0069] Test soil: fluvo-aquic soil

[0070] Experimental design: Trichoderma pleuratus HF01 fermentation broth seed-dressing cotton with different dilutions (10 times, 20 times, 30 times) was used as a demonstration, and the substrate medium-dressed cotton was used as a control (CK), and each treatment was performed in triplicate. The plot area is 20m2, randomly distributed.

[0071] Fertilization amount ...

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Abstract

The invention discloses a salt-tolerant trichoderma pleuroticola strain and application thereof, belonging to the technical field of dephosphorization microbial fertilizers. The strain is trichoderma pleuroticola HF01 and has the collection number of CGMCC (China General Microbiological Culture Collection Center) No. 8127. The invention further discloses a microbial agent containing the strain and a preparation method thereof. Shown by experiments, the trichoderma pleuroticola strain disclosed by the invention has the characteristics that the growth speed is high, the spore yield is large, the salt tolerance is strong, the stress resistance is strong, the rapid and large-number colonization at the rhizosphere of plants can be achieved, and the like; the strain has broad application prospects in the aspects of increasing the phosphate fertilizer utilization ratio, improving the soil structure and increasing the yield and quality of crops.

Description

technical field [0001] The invention belongs to the technical field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a salt-tolerant Trichoderma Pleurotus capable of efficiently dissolving phosphorus and its use, and a microbial agent prepared by using the Trichoderma Pleurotus. Background technique [0002] Phosphorus is one of the three major nutritional elements necessary for plant growth and development. Phosphorus is the basic constituent element of many organic compounds such as genetic material DNA and energy material ATP in plant cells. At the same time, photosynthesis of plants and biochemical metabolic processes in the body must have Phosphorus plays an irreplaceable role in the whole life process of plants. Phosphorus is not only an important material guarantee for agricultural production, but also a non-renewable mineral resource. In my country, 74% of the arable land is deficient in phosphorus, and the seasonal uti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00C09K17/00A01N63/04A01P1/00A01P3/00C12R1/885C09K101/00
Inventor 牛赡光刘幸红王清海段春华郑香军周峰田龙军
Owner SHANDONG FOREST SCI RES INST
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