Broad-spectrum high-sensitivity high-flux biochemical sensor and sensing method thereof

Abstract

The invention provides a wide-spectrum, high-sensitivity and high-flux biochemical sensor and a sensing method thereof. The wide-spectrum, high-sensitivity and high-flux biochemical sensor comprises a broadband light source, a container, a metal particle layer, an array type detection chip and a calculation unit connected with the array type detection chip, the method comprises the following steps: detecting the intensity of light which is emitted from each part of a metal particle layer, is subjected to localized surface plasma resonance, scattering, diffraction and interference effects and then is irradiated at each pixel element position, substituting the intensity into an augmented matrix of a matrix equation, and solving the matrix equation; the refractive index change or the physical property change of the solution to be detected or the gas to be detected is detected according to whether the obtained calculation result is changed or not compared with the initial calculation result. The technical problems that an existing biochemical sensor is large in size, a mark needs to be added for detection, manufacturing is complex, cost is high, flux is low, sensitivity is low, the detection wave band is narrow, the detection object is single, and real-time detection cannot be achieved are solved.

Description

technical field [0001] The invention relates to a wide-spectrum, high-sensitivity, high-throughput biochemical sensor and a sensing method thereof, and relates to the technical field of biochemical sensing. Background technique [0002] In the fields of medical analysis, environmental monitoring, biotechnology, pharmaceutical manufacturing and food processing, it is often necessary to detect and sense some special chemical or biological substances. However, if the traditional chemical method is used for detection and sensing, it is necessary to add a label to the chemical solution of the object to be tested, but adding a label will contaminate the original chemical solution and even affect the accuracy of detection. Therefore, people prefer to use The label-free method can understand the physical property changes of the solution or gas to be measured in real time. When the physical properties of the solution or gas to be tested change, the original chemical composition of t...

AI Technical Summary

Problems solved by technology

[0005] Technical problem: The object of the present invention is to provide a biochemical sensor and its sensing method that are miniaturized, label-free, wide-spectrum, high-sensitivity, high-throughput, multi-measuring, and capable of real-time detection, which solves the problems in the background technology. The biochemical sensor has technical problems such as large volume, labeling required for detection, complex production, high cost, low throughput, low sensitivity, narrow detection band, relatively single detection object, and incapable of real-time detection.

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