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Electrophoresis buffer solution

A technology of electrophoresis buffer and nitric acid solution, which is applied in the field of biochemistry, can solve the problems of difficult judgment of positive results, limited purchase quantity, and large demand for experimental drugs, so as to achieve easy distinction, reduce the probability of production, and high experimental success rate Effect

Pending Publication Date: 2022-05-27
HEBEI NORTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, barbiturate and barbital sodium are not easy to buy. On the one hand, it is due to the strict control of special drugs, and on the other hand, there is a large demand for experimental drugs and the purchase volume is limited.
Furthermore, barbiturate-barbital sodium buffer is easy to form crystals at both ends of the electrode during the experiment, which affects the efficiency of current mobility, making it difficult to judge positive results.

Method used

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Examples

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Embodiment 1

[0021] The electrophoresis buffer of this embodiment is prepared by adjusting the pH to 8.3-8.6 from the following raw materials: tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water.

[0022] The dosage ratio of the tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water is 10.800g: 0.930g: 1.317g: 1L; nitric acid solution is used to adjust the pH, and the mass fraction of the nitric acid solution is 50%.

[0023] The preparation method of the electrophoresis buffer of the present embodiment comprises the following steps:

[0024] The tris-base with a mass of 10.800g, disodium EDTA with a mass of 0.930g and NaCl with a mass of 1.317g were added into distilled water with a volume of 1L and fully dissolved to obtain a preparatory solution. The pH value of the preparative solution was adjusted with a nitric acid solution. Adjust to 8.3 to prepare THE running buffer.

[0025] The cellulose acetate film electrophoresis e...

Embodiment 2

[0053]The electrophoresis buffer of this embodiment is prepared by adjusting the pH to 8.3-8.6 from the following raw materials: tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water.

[0054] The dosage ratio of the tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water is 10.800g: 0.930g: 1.317g: 1L; nitric acid solution is used to adjust the pH, and the mass fraction of the nitric acid solution is 50%.

[0055] The preparation method of the electrophoresis buffer of the present embodiment comprises the following steps:

[0056] The mass of 10.800g of tris-alkali, the mass of 0.930g of disodium EDTA and the mass of 1.317g of NaCl were added to distilled water with a volume of 1L to obtain a preparatory solution, and the pH of the preparatory solution was adjusted to nitric acid solution. 8.6, prepare THE running buffer.

[0057] Using the electrophoresis buffer prepared in this example and the pH 8.6 barbital-barbi...

Embodiment 3

[0112] The electrophoresis buffer of this embodiment is prepared by adjusting the pH to 8.3-8.6 with the following raw materials: tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water.

[0113] The dosage ratio of the tris-hydroxymethylaminomethane (tris-base), disodium EDTA, NaCl and distilled water is 10.800g: 0.930g: 1.317g: 1L; nitric acid solution is used for pH adjustment, and the mass fraction of the nitric acid solution is 50%.

[0114] The preparation method of the electrophoresis buffer of the present embodiment comprises the following steps:

[0115] The mass of 10.800g of tris-alkali, the mass of 0.930g of disodium EDTA and the mass of 1.317g of NaCl were added to 1L of distilled water to fully dissolve to obtain a preparatory solution, which was adjusted to pH value with a nitric acid solution. Adjust to 8.4 to prepare THE running buffer.

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Abstract

The invention provides an electrophoretic buffer solution. The electrophoretic buffer solution is prepared by adjusting the pH value of the following raw materials to 8.3-8.6: tris (hydroxymethyl) aminomethane, EDTA (ethylene diamine tetraacetic acid) disodium, NaCl and distilled water. When the electrophoresis buffer solution prepared by the invention is used for a serum acetate fiber film electrophoresis experiment, the probability that five strips appear is high, a zone is clear and visible, and zoning is obvious; the prepared buffer solution is low in ionic strength and alkaline, the pH of the buffer solution is easy to adjust, the ionic strength is easy to calculate, and the buffer solution is suitable for carrying out all electrophoresis experiments replacing barbital-barbital sodium. In addition, the electrophoretic buffer solution prepared by the invention can be used in other life science experiment researches with different pH values and different ionic strengths as required.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, in particular to an electrophoresis buffer. Background technique [0002] Biochemistry is an important professional basic course for medicine, animal medicine and production majors in general colleges and universities, and it is an experiment-based subject. The experimental teaching focuses on the qualitative and quantitative analysis techniques of macromolecular substances. One of the important and basic experimental techniques is electrophoresis. [0003] Especially in electrophoresis experiments, cellulose acetate film electrophoresis is simple, intuitive and practical, with the characteristics of small electroosmosis, fast separation speed, clear zone, simple operation and small sample consumption. One of the most basic teaching programs in This method utilizes proteins as ampholytes. In a certain pH environment, the amount of charge on the surface is different. In addition, the size ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447B01D57/02
CPCG01N27/447B01D57/02
Inventor 张丽云赵泽鹏
Owner HEBEI NORTH UNIV