348 results about "Tris hydroxymethylaminomethane" patented technology

The invention discloses preparation and application of magnetic nano-composite material gamma-Fe2O3/PDA (Polydopamine)-GA (Gallic Acid). The preparation comprises the following steps of carrying out a hydrothermal reaction and calcinations to obtain a gamma-Fe2O3 nano particle carrier by taking FeCl3.6H2O with low price as an iron source, ethylene glycol (EG) as a reducing agent, polyvinylpyrrolidone (PVP) as a dispersing agent and a protective agent, and natrium aceticum (NaAc) as an alkali source; then wrapping a layer of polydopamine (PDA) in a buffer solution of tris (hydroxymethyl) aminomethane-hydrochloric acid (Tris-HCl) with pH of 8.5; and finally introducing gallic acid (GA) to an ethanol system by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and n-hydroxysuccinimide as cross-linking agents, wherein a specific synthetic schematic diagram is seen in attached drawing 1. Then the adsorption effects of the synthesized magnetic nano-particle on metal ions Cu<2+> and Pb<2+> are discussed, the detection effects of a glassy carbon electrode modified by the magnetic nano-particle on the metal ions Cu<2+> and Pb<2+> are researched. Results show that gamma-Fe2O3/PDA-GA has excellent detection and adsorption effects on heavy metal ions in a water environment.

The invention belongs to the field of nanotechnology, and particularly relates to a synthetic method and application of a polydopamine-modified carbon nanotube composite material. The synthetic method comprises the following steps in sequence: dispersing carbon nanotubes into dopamine-containing aqueous solution; quickly adding trismetyl aminomethane buffer solution into the dopamine-containing aqueous solution in which the carbon nanotubes are uniformly dispersed under magnetically stirring; mechanically stirring for 10 hours at room temperature; washing by water; and centrifugally separating to obtain the carbon nanotube composite material of which the surface is modified by polydopamine, wherein the carbon nanotube composite material shows high dispersibility in water. The polydopamine-modified carbon nanotube composite material adopts the carbon nanotubes as the framework, and therefore, a large specific surface area is provided; the polydopamine-modified carbon nanotube composite material is excellent in environmental stability and biocompatibility and high in dispersibility in water; the synthetic method is simple and low in cost; and the polydopamine-modified carbon nanotube composite material can be used as the substrate for analyzing metabolite micromolecule under high-throughput MALDI-TOFMS (Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry).

The invention relates to the technical field of preparation of nanometer materials, and discloses a functionalized polydopamine derived carbon layer coated carbon substrate preparation method and application. The preparation method comprises the following steps: 1) immersing a carbon substrate into a trihydroxymethyl aminomethane aqueous solution, the pH value of which is adjusted to be alkaline, and then, reacting with dopamine hydrochloride to obtain a polydopamine coated carbon substrate; 2) carrying out high-temperature carbonization on the obtained polydopamine coated carbon substrate to obtain a polydopamine derived carbon layer coated carbon substrate; and 3) enabling the obtained polydopamine derived carbon layer coated carbon substrate to react with a bimetallic saline solution to obtain a functionalized polydopamine derived carbon layer coated carbon substrate. The functionalized polydopamine derived carbon layer coated carbon substrate is stable in structure, is high in electrochemical activity, and has a series of advantages of high specific capacitance and good rate capability and the like when serving as the electrode material of a super capacitor; and the preparation method thereof is simple and easy to operate.

The invention relates to a preparation method for a hydrophilic antibacterial ultrafiltration membrane. The method comprises the following steps of providing a basic filter membrane; dissolving dopamine into a trihydroxymethyl aminomethane buffer solution to prepare a dopamine solution; coating the surface of the basic filter membrane with the dopamine solution to form a polydopamine coating on the surface of the basic filter membrane; coating the surface of the polydopamine coating with an amino-modified polyethylene glycol solution to form an amino-modified polyethylene glycol layer on the surface of the polydopamine coating; arranging antibacterial nanoparticles on the surface of the amino-modified polyethylene glycol layer to obtain the hydrophilic antibacterial ultrafiltration membrane.

The invention provides a pharmaceutical formulation comprising:

(1) THAM, which is tris(hydroxymethyl)aminomethane, as a selective absorbefacient to enhance through the nasal mucosa-lined epithelium the absorption of substances of peptide nature; and

(2) a therapeutically effective amount of active nasal peptide, its pharmaceutically acceptable salt or its peptidic fragment; in a pharmaceutically acceptable, aqueous liquid diluent or carrier, said formulation being in a form suitable for nasal administration.

The invention discloses a method for modifying polytetrafluoroethylene-aramid blended fabric jointly by dopamine-polyethyleneimine-nanoparticles; the method includes: adding polytetrafluoroethylene-aramid blended fabric, dopamine hydrochloride, polyethylenimine and nanoparticles into trihydroxymethyl-aminomethane/hydrochloride buffered solution, stirring, ultrasonically dispersing well, and continuously stirring at room temperature to allow for 2-24 h of reaction; after the reaction, taking out the blended fabric, sufficiently washing with deionized water to remove unreacted impurities on thesurface, and then drying in a vacuum oven to obtain the surface-modified polytetrafluoroethylene-aramid blended fabric. An organic-inorganic hybrid functional coating is constructed on the fiber surface through modifying; therefore, both active functional groups and greater surface roughness are imparted to the surface of the blended fabric, and the interface adhesion performance between the blended fabric and phenolic resin matrix can be improved through chemical bonding and mechanical interlocking.

The invention discloses an optimized in vitro cell-free protein synthesis system. The system comprises a cell extract, a carbohydrate material, a phosphate compound, a buffering agent and a DNA molecular template for encoding an exogenous protein, wherein the cell extract is a yeast cell extract inserted into a T7 RNA polymerase gene; the carbohydrate material is a mixture of glucose and maltodextrin; the buffering agent is a trihydroxymethyl aminomethane buffering agent; and the DNA molecule template is prepared by a nucleic acid isothermal amplification method, and a sequence as shown in SEQID NO.1 is inserted into the upstream of the coding sequence of the exogenous protein in the DNA molecular template. By optimizing, the cost of in vitro protein synthesis is reduced and the yield ofthe target protein is increased.

The invention discloses liquid-based thin-layer cell preservation solution and use thereof. The preservation solution comprises 5 to 28 volume percent of aldehyde solution, 0.1 to 3 volume percent of acid solution, 0.1 to 6 mass volume percent of chloride, 0.1 to 3 mass volume percent of tris(hydroxymethyl)aminomethane and the balance volume percent of double distilled water. The preservation solution of the invention can remove red blood cells, preserve white blood cells, timely fix the original state of cells of diagnostic value, soften mucilage and epidermal tissues, weaken mucous effect and separate effective cells from mucilage. The microscopic examination piece manufactured by using the preservation solution has clear microscopic examination view field and high cellular staining contrast and is widely suitable for gynecological and non-gynecological exfoliative cytological specimens and manual, semi-automatic or fully-automatic liquid-based cell substrate making processes. The production cost of the preservation solution is low, so the application and promotion of a liquid-based thin-layer cell detection technique are convenient.

This invention relates to novel tris(hydroxymethyl)aminomethane, choline and N-methyl-D-glucamine salt forms of the following Compound (1) and methods for the preparation thereof, pharmaceutical compositions thereof, and their use in the treatment of Hepatitis C Viral (HCV) infection:

The invention discloses a zinc-cobalt sulfide/carbon nano negative electrode material and a preparation method thereof. The negative electrode material is of a hollow irregular spherical shell structure formed by distributing zinc-cobalt sulfide nano particles in a carbon matrix and on the surface of the carbon matrix, wherein the mass ratio of the zinc-cobalt sulfide to the carbon is (0.5 to 4.0): 1. The method comprises the steps of (1) adding a surfactant and 2-methylimidazole into an alcoholic solution, stirring, adding a zinc source alcoholic solution, stirring, standing and centrifuging;(2) dissolving in an alcoholic solution, adding a surfactant and 2-methylimidazole, stirring, adding a cobalt source alcoholic solution, stirring, carrying out solvothermal reaction, cooling, centrifuging, washing and drying; (3) adding trihydroxymethyl aminomethane into an alcoholic solution, stirring, adding dopamine hydrochloride, stirring, carrying out suction filtration, washing and drying;and (4) pre-roasting, cooling, mixing with sulfur powder, roasting and cooling. The material provided by the invention is excellent in electrochemical performance. The method provided by the inventionis mild, low in cost, environmentally friendly and suitable for industrial production.

A novel coronavirus earlier-stage screening method disclosed by the invention can be used for detecting the novel coronavirus, and is particularly used for screening the SARS-CoV-2IgA antibody in theearly stage. A kit comprises an SARS-CoV-2 magnetic bead coating material used as R1, containing a magnetic bead coating material trihydroxymethyl aminomethane buffer solution coated by an SARS-CoV-2recombinant antigen, wherein the pH value is 7.1-7.4; and an acridinium ester marker of the anti-human IgA antibody as R2, wherein the acridinium ester marker is a 2-(N-morpholine) ethanesulfonic acidbuffer solution containing an acridinium ester labeled mouse anti-human IgA monoclonal antibody. The kit and the SARS-CoV-2IgA antibody immunoassay reagent disclosed by the invention can be used forsupplementing and diagnosing new coronal pneumonia at an earlier stage, and have the characteristic of accurate detection result.

The invention relates to a preparation method of modified activated carbon fiber for adsorbing ultra-micro sulfur dioxide, belonging to the technical field of gas adsorption and separation. The method comprises the following steps: taking viscose-based activated carbon fiber which is oxidized by dilute nitric acid as a matrix, and taking tris hydroxyl methyl aminomethan, alanine, serine and arginine as modifiers; and successfully preparing chemically modified activated carbon fibers in which the surfaces are modified by much amidogen through a hydro-thermal reaction under certain conditions. Compared with the prior art, the preparation method has the following advantages: (1) a micromolecule modifier is used for chemically modifying the surface to keep the structural advantage of the activated carbon fibers such as high specific surface and more micro pores so that the activated carbon fibers simultaneously have abundant micro-pore structures and high activity reaction surfaces to represent relatively longer penetrating time and larger saturation adsorption capacity; (2) the modifying method is temperate in condition, small in pollution, energy-saving and environment-friendly, and easy for scaled production; and (3) the modified absorbing capacity can be increased to that 1 g of the absorbent can absorb 40-60 mg of SO2, which is improved by 4-6 times compared with the absorbing capacity of the absorbent before the modification.

The invention discloses a photo-thermal conversion material, which has a double-layer structure, wherein the double-layer structure is a polydopamine layer on a polyurethane sponge layer, the top layer of the double-layer structure is dark black, the thickness of the top layer is 0.5-2.0 mm, and the total thickness of the double-layer structure is 10-25 mm. The preparation method of the photo-thermal conversion material comprises: S1, purifying polyurethane sponge; S2, loading polydopamine: immersing the polyurethane sponge purified in the step S1 into a container filled with a hydrochloric acid-containing dopamine trihydroxymethyl aminomethane buffer solution (Tris-HCl buffer solution), and carrying out a water bath reaction for 4-24 h at a temperature of 25-80 DEG C without any disturbance condition and container sealing to obtain modified sponge; and S3, drying. The invention also provides applications of the photo-thermal conversion material. According to the invention, with the photo-thermal conversion material, the problems of low efficiency, high cost, complex process and the like of the existing solar steam photo-thermal conversion are solved.

The invention discloses a preparation and an application of an Fe3O4@C@MoS2 composite material with a core-shell structure. The preparation method includes the following steps: S1. adding FeCl3 and NaOH into water, uniformly mixing, loading the mixed solution into a polytetrafluoroethylene stainless steel autoclave, and reacting to obtain uniform cubic Fe2O3 particles; S2, adding the Fe2O3 particles prepared in S1 and dopamine hydrochloride into a trimethylolaminomethane buffer solution, stirring and reacting at room temperature with a magnetic stirrer, then washing the reaction product, and collecting an Fe2O3@PDA composite with a core-shell structure through a centrifugal method; S3. adding the Fe2O3@PDA prepared in S2, ammonium molybdate tetrahydrate and thiourea into water in sequenceand stirring uniformly, washing the product after the reaction is finished, and collecting Fe2O3@PDA@MoS2 composite through the centrifugal method; and S4. calcining the Fe2O3@PDA@MoS2 composite underthe flow of hydrogen and argon to finally obtain the Fe3O4@C@MoS2 composite. The Fe3O4@C@MoS2 composite material prepared by the method has excellent microwave absorption performance.

The invention discloses a preparation method of a frozen diluent of sheep semen and an application of the preparation method. The preparation method of the frozen diluent of the sheep semen comprisesthe following steps: firstly, accurately weighing tris(hydroxymethyl) aminoethane, dihydrate trisodium citrate, fructose, an inositol compound, ethanediol or glycerinum, and oxytocin, adding the materials into ultrapure water, dissolving, adding a fresh yolk centrifuged liquid supernatant, penicillin and streptomycin, mixing evenly, fixing the volume after adjusting the pH value to 6.8-7.2, thus obtaining the frozen diluent of the sheep semen. The inositol compound is used to replace an antifreeze protein, the defect of the application of the antifreeze protein is avoided, meanwhile, the oxytocin is added when the semen is conveyed, and the sperm vitality and the acrosomal integrity are improved, so that the fertility rate is increased.

The invention discloses pegylation polyoxometallate and preparation method thereof. According to the invention, polyethylene glycol and polyoxometallate are connected by utilizing a covalent bond mode, and a pegylated tungsten-containing polyoxometallate hybrid polymer is obtained. The method comprises the following synthesis steps: 1) reacting polyethylene glycol with succinic anhydride to obtain carboxyl polyethylene glycol; 2) carrying out an amidation reaction between the carboxyl polyethylene glycol and trihydroxymethyl aminomethane to obtain trihydroxymethyl polyethylene glycol; and 3) reacting the trihydroxymethyl polyethylene glycol and the tungsten-containing polyoxometallate to obtain the hybrid compound. The pegylated polyoxometallate hybrid compound is synthesized in the covalent bond mode. According to the synthetic method, a novel organic-inorganic hybrid compound can be obtained, the preparation method is easy and feasible, and the reaction process is easy to control, simple in purification and high in yield. According to the hybrid compound, the excellent performances of the inorganic polyoxometallate and organic polyethylene glycol are kept, so that the hybrid molecule has potential application values in the aspects of catalysis, materials, medicines and the like.

The invention provides a method for detecting cervical fluid heme. The method comprises the following steps of: wiping at the cervical part with little pressure to obtain the fluid; processing the fluid with a tri-hydroxymethyl aminomethane-hydrochloric acid buffer solution with a pH value of 7.4; and then reacting with a color producing reagent, comparing with a shade guide and recording the detection result. The method provided by the invention is simple and practical and low in cost, and can quickly screen the sample without heme, the sample with heme and the sample possibly with heme in the cervical fluid, thereby providing preparation for further screening. The invention also relates to a detection kit for detecting the cervical fluid heme.

The invention discloses preparation of a fibrous catalyst loaded with metal nanoparticles and application. The preparation method comprises the following steps: adding metal salt into a solution system containing trihydroxy methyl aminomethane, fiber and dopamine hydrochloride to obtain mixed solution, then stirring, polymerizing dopamine to obtain polydopamine, wrapping fiber with the polydopamine, and simultaneously loading the metal nanoparticles; then, carrying out heat annealing treatment under the atmosphere of inertial gas to prepare a fiber@nitrogen-doped carbon metal compound material, namely the fibrous catalyst loaded with metal nanoparticles. The preparation and the application disclosed by the invention have the beneficial effects that by setting of a whole process flow of thepreparation method and improvement on reaction conditions and parameters of all key process steps, the activity and the cycling stability of the loaded nano-metal catalyst can be effectively improved.

A polyacrylamide gel utilising a buffer system comprising Tris(hydroxymethyl)aminomethane at the concentration range 0.15 to 0.25 M titrated with hydrochloric acid to a pH between 6.5 and 7.5. The gel is substantially stable to polyacrylamide hydrolysis during storage for at extended periods and has an acceptable shelf-life of at least 6 months after storage at about 4° C.

The invention discloses a modified polymer film as well as a preparation method and application thereof, and particularly relates to the field of modified polymer films. The preparation method comprises the following steps: dropwise adding a sulfanilic acid diazonium salt solution into Na<+> intercalated MXene, and after reaction, centrifuging, filtering, washing, ultrasonically treating and freeze-drying to obtain sulfonated MXene powder; immersing a polymer film in a GA/polyethyleneimine (PEI) mixed solution containing trihydroxymethyl aminomethane and metal salt, and reacting to obtain a GAcoating modified polymer film; and depositing the sulfonated MXene powder on the GA coating modified polymer film, and repeating the operations to obtain the single-layer or multi-layer GA/MXene modified polymer film. According to the invention, two methods, namely vacuum assisted suction filtration and layer-by-layer self-assembling, are combined, and the method has the characteristics of uniform film coating, controllable film thickness, better stability, low energy consumption and easiness in operation. In addition, the prepared modified polymer film has the functions of efficiently removing medium and small molecular toxins and having good oxidation resistance and protein adsorption resistance.

The invention discloses a carbon-coated stannous oxide compound. The carbon-coated stannous oxide compound is prepared by the steps of dissolving trihydroxymethyl aminomethane into water, stirring and preparing into a buffer solution; weighing the buffer solution, adding stannous oxide, stirring to form a black turbid solution; adding dopamine and continuously stirring; performing washing, centrifuging and drying; and performing calcining in nitrogen atmosphere to obtain the carbon-coated stannous oxide composite material. Dopamine is used as the carbon source for coating the surface of SnO, so that volume expansion of SnO in a circulation process is relieved, thereby improving the cycling performance in the application when SnO is used as a negative electrode material of a sodium ion battery; in the synthesis process, the solvent is non-toxic and harmless, the cost is relatively low and the operation is simple; the carbon-coated stannous oxide composite material has the most major advantage of excellent cycling performance; the initial-circle charging specific capacity can be close to 500mAh/g and the capacity is barely attenuated after 300 cycles, so that possibility is provided for the actual application of the composite material in the sodium ion battery material in the future.