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Method for predicting immune dysfunction of recurrent abortion population during pregnancy

A technology for recurrent miscarriage and immune function, applied in the field of genetics, can solve the problems of lack of population research data, undiscovered population research and exploration, etc., and achieve the effect of improving predictive ability

Pending Publication Date: 2022-06-03
天津大学温州安全(应急)研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no relevant population research and exploration have been found, and there is a lack of corresponding population research data

Method used

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  • Method for predicting immune dysfunction of recurrent abortion population during pregnancy
  • Method for predicting immune dysfunction of recurrent abortion population during pregnancy
  • Method for predicting immune dysfunction of recurrent abortion population during pregnancy

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Experimental program
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Embodiment

[0016] A method for predicting immune dysfunction during pregnancy in a population with recurrent miscarriage, comprising the following:

[0017] Platelets from plasma were separated by differential centrifugation, then the platelet pellet was digested with nuclear genomic DNA interference using DNA restriction enzymes, followed by direct bisulfite treatment of the digested product, combined with pyrosequencing Pyrosequencing was used to detect the methylation levels of respiratory chain-related genes (MT-ATP8, MT-COX1, MT-COX2, MT-COX3, MT-ND5, MT-TRNA) in platelet mtDNA.

[0018] As a new sequence analysis technology, pyrosequencing technology can rapidly detect the frequency of methylation, and perform accurate qualitative and quantitative detection of methylation sites in samples. By accurately quantifying methylation frequencies at individual contiguous CpG sites, pyrosequencing itself can detect and quantify subtle changes in methylation levels. During sequence extensio...

experiment example

1 Basic characteristics of the study population

2 The relationship between a single platelet mtDNA locus and abnormal immune function during pregnancy

In the follow-up population, the single platelet mtDNA loci associated with immune dysfunction are shown in Table 2, involving a total of 18 loci (ATP8p1, ATP8p2, ATP8, COX1p1, COX1p2, COX1, COX2p1, COX2p2, COX2, COX3p1, COX3p2, COX3, ND5p1, ND5p2, ND5, TRNAp1, TRNAp2, TRNA). The results showed that there was no significant statistical significance between the immune abnormalities during pregnancy and the above single platelet mtDNA locus (p>0.05).

[0027] 3 Traditional risk factors and multiple platelet mtDNA loci combined to predict immune dysfunction during pregnancy

It can be seen from the above results that the change of the methylation level of a single platelet mtDNA site has a relatively weak effect on predicting the abnormal immune function during pregnancy. Therefore, the present invention combines multipl...

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Abstract

The invention discloses a method for predicting immune dysfunction of a recurrent abortion population during pregnancy, which comprises the following steps: separating platelets in plasma of URSA patients by using a differential centrifugation method, then carrying out nuclear genome DNA interference treatment digestion on platelet precipitation by using DNA restriction enzyme, then directly carrying out bisulfite treatment on a digestion product, and finally predicting immune dysfunction of the recurrent abortion population during pregnancy. A pyrosequencing method is combined to detect the methylation level of respiratory chain related gene loci, including MT-ATP8, MT-COX1, MT-COX2, MT-COX3, MT-ND5 and MT-TRNA, in the platelet mtDNA; the method has the advantages that the methylation level is detected by selecting the mitochondrial sites related to URSA, and the diagnosis efficiency of the biomarker is evaluated by using AUC, so that the prediction capability on the onset risk of the blood coagulation dysfunction is improved.

Description

technical field [0001] The invention relates to a method for predicting abnormal immune function, in particular to a method for predicting abnormal immune function during pregnancy in a population of recurrent miscarriage, and belongs to the field of genetics. Background technique [0002] Spontaneous abortion accounts for 15%-40% of all pregnancies, and 2 or more consecutive spontaneous abortions are called recurrent spontaneous abortion (RSA). The factors that cause RSA are complex and diverse, including genetic factors, infectious factors, Endocrine factors, anatomical factors, immune factors and coagulation factors. Among them, 40% to 50% of patients have unexplained miscarriage, which is called unexplained recurrent miscarriage (URSA). Abnormal coagulation function is considered to be the main cause of RSA. Studies have shown that nearly 55%-62% of patients with adverse pregnancy outcomes have abnormal coagulation function, usually manifested as hypercoagulable state ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/154
Inventor 郭丽琼张颖刘子泉刘琪思婧于媛媛
Owner 天津大学温州安全(应急)研究院
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